LOCUS       sequence-85806-e        4006 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 433, Emission: 475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4006)
  AUTHORS   Markwardt ML, Kremers GJ, Kraft CA, Ray K, Cranfill PJ, Wilson KA, 
            Day RN, Wachter RM, Davidson MW, Rizzo MA
  TITLE     An improved cerulean fluorescent protein with enhanced brightness 
            and reduced reversible photoswitching.
  JOURNAL   PLoS One. 2011  . 6(3):e17896.
  PUBMED    21479270
REFERENCE   2  (bases 1 to 4006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2011  . 6(3):e17896."
COMMENT     Sequence Label: mCerulean3-Alpha-5-Integrin-12
FEATURES             Location/Qualifiers
     source          1..4006
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      3241..3250
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3247..3249,3250..3252,3253..3966)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIK
                     ANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3292..3313)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3553..3572)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3900..3921
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gctagcgcta ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc
       61 accatgggga gccggacgcc agagtcccct ctccacgccg tgcagctgcg ctggggcccc
      121 cggcgccgac ccccgctgct gccgctgctg ttgctgctgc tgccgccgcc acccagggtc
      181 gggggcttca acttagacgc ggaggcccca gcagtactct cggggccccc gggctccttc
      241 ttcggattct cagtggagtt ttaccggccg ggaacagacg gggtcagtgt gctggtggga
      301 gcacccaagg ctaataccag ccagccagga gtgctgcagg gtggtgctgt ctacctctgt
      361 ccttggggtg ccagccccac acagtgcacc cccattgaat ttgacagcaa aggctctcgg
      421 ctcctggagt cctcactgtc cagctcagag ggagaggagc ctgtggagta caagtccttg
      481 cagtggttcg gggcaacagt tcgagcccat ggctcctcca tcttggcatg cgctccactg
      541 tacagctggc gcacagagaa ggagccactg agcgaccccg tgggcacctg ctacctctcc
      601 acagataact tcacccgaat tctggagtat gcaccctgcc gctcagattt cagctgggca
      661 gcaggacagg gttactgcca aggaggcttc agtgccgagt tcaccaagac tggccgtgtg
      721 gttttaggtg gaccaggaag ctatttctgg caaggccaga tcctgtctgc cactcaggag
      781 cagattgcag aatcttatta ccccgagtac ctgatcaacc tggttcaggg gcagctgcag
      841 actcgccagg ccagttccat ctatgatgac agctacctag gatactctgt ggctgttggt
      901 gaattcagtg gtgatgacac agaagacttt gttgctggtg tgcccaaagg gaacctcact
      961 tacggctatg tcaccatcct taatggctca gacattcgat ccctctacaa cttctcaggg
     1021 gaacagatgg cctcctactt tggctatgca gtggccgcca cagacgtcaa tggggacggg
     1081 ctggatgact tgctggtggg ggcacccctg ctcatggatc ggacccctga cgggcggcct
     1141 caggaggtgg gcagggtcta cgtctacctg cagcacccag ccggcataga gcccacgccc
     1201 acccttaccc tcactggcca tgatgagttt ggccgatttg gcagctcctt gacccccctg
     1261 ggggacctgg accaggatgg ctacaatgat gtggccatcg gggctccctt tggtggggag
     1321 acccagcagg gagtagtgtt tgtatttcct gggggcccag gagggctggg ctctaagcct
     1381 tcccaggttc tgcagcccct gtgggcagcc agccacaccc cagacttctt tggctctgcc
     1441 cttcgaggag gccgagacct ggatggcaat ggatatcctg atctgattgt ggggtccttt
     1501 ggtgtggaca aggctgtggt atacaggggc cgccccatcg tgtccgctag tgcctccctc
     1561 accatcttcc ccgccatgtt caacccagag gagcggagct gcagcttaga ggggaaccct
     1621 gtggcctgca tcaaccttag cttctgcctc aatgcttctg gaaaacacgt tgctgactcc
     1681 attggtttca cagtggaact tcagctggac tggcagaagc agaagggagg ggtacggcgg
     1741 gcactgttcc tggcctccag gcaggcaacc ctgacccaga ccctgctcat ccagaatggg
     1801 gctcgagagg attgcagaga gatgaagatc tacctcagga acgagtcaga atttcgagac
     1861 aaactctcgc cgattcacat cgctctcaac ttctccttgg acccccaagc cccagtggac
     1921 agccacggcc tcaggccagc cctacattat cagagcaaga gccggataga ggacaaggct
     1981 cagatcttgc tggactgtgg agaagacaac atctgtgtgc ctgacctgca gctggaagtg
     2041 tttggggagc agaaccatgt gtacctgggt gacaagaatg ccctgaacct cactttccat
     2101 gcccagaatg tgggtgaggg tggcgcctat gaggctgagc ttcgggtcac cgcccctcca
     2161 gaggctgagt actcaggact cgtcagacac ccagggaact tctccagcct gagctgtgac
     2221 tactttgccg tgaaccagag ccgcctgctg gtgtgtgacc tgggcaaccc catgaaggca
     2281 ggagccagtc tgtggggtgg ccttcggttt acagtccctc atctccggga cactaagaaa
     2341 accatccagt ttgacttcca gatcctcagc aagaatctca acaactcgca aagcgacgtg
     2401 gtttcctttc ggctctccgt ggaggctcag gcccaggtca ccctgaacgg tgtctccaag
     2461 cctgaggcag tgctattccc agtaagcgac tggcatcccc gagaccagcc tcagaaggag
     2521 gaggacctgg gacctgctgt ccaccatgtc tatgagctca tcaaccaagg ccccagctcc
     2581 attagccagg gtgtgctgga actcagctgt ccccaggctc tggaaggtca gcagctccta
     2641 tatgtgacca gagttacggg actcaactgc accaccaatc accccattaa cccaaagggc
     2701 ctggagttgg atcccgaggg ttccctgcac caccagcaaa aacgggaagc tccaagccgc
     2761 agctctgctt cctcgggacc tcagatcctg aaatgcccgg aggctgagtg tttcaggctg
     2821 cgctgtgagc tcgggcccct gcaccaacaa gagagccaaa gtctgcagtt gcatttccga
     2881 gtctgggcca agactttctt gcagcgggag caccagccat ttagcctgca gtgtgaggct
     2941 gtgtacaaag ccctgaagat gccctaccga atcctgcctc ggcagctgcc ccaaaaagag
     3001 cgtcaggtgg ccacagctgt gcaatggacc aaggcagaag gcagctatgg cgtcccactg
     3061 tggatcatca tcctagccat cctgtttggc ctcctgctcc taggtctact catctacatc
     3121 ctctacaagc ttggattctt caaacgctcc ctcccatatg gcaccgccat ggaaaaagct
     3181 cagctcaagc ctccagccac ctctgatgcc acggtaccgc gggcccggga tccaccggtc
     3241 gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     3301 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
     3361 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     3421 cccaccctcg tgaccaccct gagctggggc gtgcagtgct tcgcccgcta ccccgaccac
     3481 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     3541 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     3601 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     3661 gggcacaagc tggagtacaa cgccatccac ggcaacgtct atatcaccgc cgacaagcag
     3721 aagaacggca tcaaggccaa cttcggcctc aactgcaaca tcgaggacgg cagcgtgcag
     3781 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     3841 aaccactacc tgagcaccca gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac
     3901 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     3961 aagtaaagcg gccgcgactc tagatcataa tcagccatac cacatg
//
