LOCUS       sequence-85809-e        3559 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Chromatin Assembly Factor 1, Excitation: 433, 
            Emission: 475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Markwardt ML, Kremers GJ, Kraft CA, Ray K, Cranfill PJ, Wilson KA, 
            Day RN, Wachter RM, Davidson MW, Rizzo MA
  TITLE     An improved cerulean fluorescent protein with enhanced brightness 
            and reduced reversible photoswitching.
  JOURNAL   PLoS One. 2011  . 6(3):e17896.
  PUBMED    21479270
REFERENCE   2  (bases 1 to 3559)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2011  . 6(3):e17896."
COMMENT     Sequence Label: mCerulean3-CAF1-C-10
FEATURES             Location/Qualifiers
     source          1..3559
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(32..34,35..37,38..748)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIK
                     ANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(77..98)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(338..357)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     685..706
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    2567..2578
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
ORIGIN
        1 gtcagatccg ctagcgctac cggtcgccac catggtgagc aagggcgagg agctgttcac
       61 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
      121 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
      181 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgagct ggggcgtgca
      241 gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
      301 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
      361 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
      421 cttcaaggag gacggcaaca tcctggggca caagctggag tacaacgcca tccacggcaa
      481 cgtctatatc accgccgaca agcagaagaa cggcatcaag gccaacttcg gcctcaactg
      541 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
      601 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtcca agctgagcaa
      661 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
      721 cactctcggc atggacgagc tgtacaagtc cggactcaga tctggcagcg gtggaggctt
      781 ggaggagccg gaggccgcga cgcggacagc cgcggccgtg gattgcaaag acagacccgg
      841 gttcccagtg aagaggttaa tacaagcccg cttgcccttc aaacgcctga accttgttcc
      901 caaggagaaa gttgaggaag acacatcacc aaaagcagct gtggaaagca aagtcccgga
      961 tctacaattg tctttgggca cttttgaaag ccagtgtcac acaggctctc acgtaggctt
     1021 gagtacaaag ttggtcggtg gccagggccc cattgatagc ttcttgaggg caacaatcaa
     1081 gccagtaccg agtgtggtca ttatcgacct gacggagaac tgcagtgaca ttccagacag
     1141 ccctgagggc cacagtgaac taagtcctga cactgctggt gtggtcacca cagtggaagg
     1201 agctgccaag cagcaggagc actctgcagc agagctgtgt ctcctagaga ccccttcaga
     1261 catcacttgt cacatggaag aggagcctgg gagtccagga gacccaaaga ggacgggtga
     1321 ctgtcaggct ggttcactac agagctgtcc ggagctgacc ccaggttcta ggacatgccc
     1381 caccaaggag ttatcaagct ggagcaaggc aggggatctc ctatttatag agaaagtgcc
     1441 tgtggtggtg ctagaggaca tcctggccac taagccctcc attgcctctc ttcccatgat
     1501 gtccctggat aggagtgtca cttcagagag tgaaatactg gagtcctgcc ccgaggatga
     1561 ctccatactg agccactcct ccaccaactc ctcttctcca accagctctc ctgagggacc
     1621 atccacaccc ccagaacatc ggggtgggag aagctccccc tccaccccag cctgcagagt
     1681 tgccaagaac tttgtcaaag gctccacaga gaagggcagg agcaaactgc acagagatag
     1741 agaacagcag agggaagaga aggaaaagct gagggaagag atacgacgag ccaaggagga
     1801 ggcacggaag aagaaggagg aggagaagga gctgaaggaa aaggagcgga gggaaaagcg
     1861 ggagaaagat gagaaggaga aggcggagaa gcagcggcta aaggaggaaa aacgcaagga
     1921 gcggcaggaa gctctggagg ctaaactgga ggagaagagg aagaaggagg aagagaagcg
     1981 gttacgggag gaagagaagc ggttacggga agaagagaag cgcattaagg cagaaaaggc
     2041 agagatcact cgattcttcc agaaaccgaa gactccgcag gctcccaaga ccctggccgg
     2101 ttcctgtggt aagtttgccc cctttgaaat caaagaacac atggtgctgg ctccacggtg
     2161 ccgagctgcc ttggatcaag acctgtgcga tcagctggac cagctcctgc agcagcagag
     2221 tgtggcaagc accttcctca gtgacctaaa aagccggctg cccttgagat cggggcccac
     2281 ccgggtctgt ggccatgaca cagacatcat gaacagggat gtggtcattg tggagagcag
     2341 caaggtggac ggtgtgtctg aaaggaagaa gtttggccgc atgaagcttc tgcagttctc
     2401 agagaaccac cggccagcat actgggggac gtggaacaag aagacagcca tcatccgccc
     2461 ccggaacccc tgggcccagg acaaagacct tcttgactat gaggtagaca gtgacgatga
     2521 gtgggaagag gaggaacctg gggagtcttt gtcccacagt gagggggatg aggatgacga
     2581 tgtgggggaa gatgaggatg aggacgatgg cttctttgtg ccccatgggt acttatctga
     2641 agatgaaggc gtgactgagg agtgtgcaga ccctgagaac cacaaagtac accagaagct
     2701 gaaggccaag gagtgggatg agctcctagc caagggcaag cgctttcgtg tgctgcagcc
     2761 tgtgcacgtg ggatgtgtat gggcatcaga ggcagccaac tgcacgagtt ccgacttgaa
     2821 gctgctgcag cagttcactg catgcctgct ggatgtggca tcccctgatg agccagaacc
     2881 cggggcctcc aggagggaaa agagggacca gcatatcctg gcccagttgc ttccgctgct
     2941 acatggcaat gtgaatggga gcaaggtgat catccatgaa ttccaggagc aatgccgcag
     3001 gggcctgctg acccttccaa gtcccacccc acacctgcag atgcccaact tggaggatgc
     3061 tgttgctgtg ccatccaagg ccaggctgaa gcgccttatc tctgagaact ctgcctatga
     3121 gaagcggccc aacttccgta tgtgctggta tgtgcaccca gaggtgctca agagctttgg
     3181 ccaggagtgc ctgccagtgc cctgccagtg gacctacatc accaccatgc cctcagcacc
     3241 cagggaagac agtggcagtg cttctactga ggggcctggc cagagcacac ctatgctact
     3301 caagcggaag ccagcggcca ccatgtgcat cacgcagttt atgaagaagc ggcgctatga
     3361 tggacaggtt gggtctgggg acatggatgg cttccaggca gacacagaag aggatgaaga
     3421 ggatgacaca gactgtatga tcatagatgt gccagatgtt gggagtgacg tgtcagaagc
     3481 tcccatacct gctcccacac tttgcaagtg aggatccacc ggatctagat aactgatcat
     3541 aatcagccat accacattt
//
