LOCUS       sequence-85811-e        2272 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane, Excitation: 433, Emission: 475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Markwardt ML, Kremers GJ, Kraft CA, Ray K, Cranfill PJ, Wilson KA, 
            Day RN, Wachter RM, Davidson MW, Rizzo MA
  TITLE     An improved cerulean fluorescent protein with enhanced brightness 
            and reduced reversible photoswitching.
  JOURNAL   PLoS One. 2011  . 6(3):e17896.
  PUBMED    21479270
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2011  . 6(3):e17896."
COMMENT     Sequence Label: mCerulean3-CD36-C-10
FEATURES             Location/Qualifiers
     source          1..2272
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(35..37,38..40,41..751)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIK
                     ANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(80..101)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(341..360)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     688..709
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggagctgtt
       61 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
      121 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
      181 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga gctggggcgt
      241 gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
      301 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
      361 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
      421 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaacg ccatccacgg
      481 caacgtctat atcaccgccg acaagcagaa gaacggcatc aaggccaact tcggcctcaa
      541 ctgcaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
      601 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccaagctgag
      661 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
      721 gatcactctc ggcatggacg agctgtacaa gtccggactc agatctggca gcagtggagg
      781 tggctgtgac cggaactgtg ggctcatcgc tggggctgtc attggtgctg tcctggctgt
      841 gtttggaggt attctaatgc cagttggaga cctgcttatc cagaagacaa ttaaaaagca
      901 agttgtcctc gaagaaggta caattgcttt taaaaattgg gttaaaacag gcacagaagt
      961 ttacagacag ttttggatct ttgatgtgca aaatccacag gaagtgatga tgaacagcag
     1021 caacattcaa gttaagcaaa gaggtcctta tacgtacaga gttcgttttc tagccaagga
     1081 aaatgtaacc caggacgctg aggacaacac agtctctttc ctgcagccca atggtgccat
     1141 cttcgaacct tcactatcag ttggaacaga ggctgacaac ttcacagttc tcaatctggc
     1201 tgtggcagct gcatcccata tctatcaaaa tcaatttgtt caaatgatcc tcaattcact
     1261 tattaacaag tcaaaatctt ctatgttcca agtcagaact ttgagagaac tgttatgggg
     1321 ctatagggat ccatttttga gtttggttcc gtaccctgtt actaccacag ttggtctgtt
     1381 ttatccttac aacaatactg cagatggagt ttataaagtt ttcaatggaa aagataacat
     1441 aagtaaagtt gccataatcg acacatataa aggtaaaagg aatctgtcct attgggaaag
     1501 tcactgcgac atgattaatg gtacagatgc agcctcattt ccaccttttg ttgagaaaag
     1561 ccaggtattg cagttctttt cttctgatat ttgcaggtca atctatgctg tatttgaatc
     1621 cgacgttaat ctgaaaggaa tccctgtgta tagatttgtt cttccatcca aggcctttgc
     1681 ctctccagtt gaaaacccag acaactattg tttctgcaca gaaaaaatta tctcaaaaaa
     1741 ttgtacatca tatggtgtgc tagacatcag caaatgcaaa gaagggagac ctgtgtacat
     1801 ttcacttcct cattttctgt atgcaagtcc tgatgtttca gaacctattg atggattaaa
     1861 cccaaatgaa gaagaacata ggacatactt ggatattgaa cctataactg gattcacttt
     1921 acaatttgca aaacggctgc aggtcaacct attggtcaag ccatcagaaa aaattcaagt
     1981 attaaagaat ctgaagagga actatattgt gcctattctt tggcttaatg agactgggac
     2041 cattggtgat gagaaggcaa acatgttcag aagtcaagta actggaaaaa taaacctcct
     2101 tggcctgata gaaatgatct tactcagtgt tggtgtggtg atgtttgttg cttttatgat
     2161 ttcatattgt gcatgcagat cgaaaacaat aaaataagaa ttctgcagtc gacggtaccg
     2221 cgggcccggg atccaccgga tctagataac tgatcataat cagccatacc ca
//
