LOCUS       sequence-85814-e        1479 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Clathrin Vesicles, Excitation: 433, Emission: 475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Markwardt ML, Kremers GJ, Kraft CA, Ray K, Cranfill PJ, Wilson KA, 
            Day RN, Wachter RM, Davidson MW, Rizzo MA
  TITLE     An improved cerulean fluorescent protein with enhanced brightness 
            and reduced reversible photoswitching.
  JOURNAL   PLoS One. 2011  . 6(3):e17896.
  PUBMED    21479270
REFERENCE   2  (bases 1 to 1479)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2011  . 6(3):e17896."
COMMENT     Sequence Label: mCerulean3-Clathrin-15
FEATURES             Location/Qualifiers
     source          1..1479
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(35..37,38..40,41..751)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIK
                     ANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(80..101)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(341..360)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     688..709
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tgacgtcaga tccgctagcg ctaccggtcg ccccatggtg agcaagggcg aggagctgtt
       61 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
      121 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
      181 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga gctggggcgt
      241 gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
      301 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
      361 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
      421 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaacg ccatccacgg
      481 caacgtctat atcaccgccg acaagcagaa gaacggcatc aaggccaact tcggcctcaa
      541 ctgcaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
      601 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccaagctgag
      661 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
      721 gatcactctc ggcatggacg agctgtacaa gtccggactc agatctcgag ctcaagcttc
      781 gaattctgca gtcgacatgg ctgatgactt tggcttcttc tcgtcgtcgg agagtggtgc
      841 cccggaggcg gcggaggagg acccggcggc cgccttcctg gcccagcagg agagcgagat
      901 tgcaggcata gagaacgacg agggcttcgg ggcacctgcc ggcagccatg cggcccccgc
      961 acagccgggc cccacgagtg gggctggttc tgaggacatg gggaccacag tcaatggaga
     1021 tgtgtttcag gaggccaacg gtcctgctga tggctacgca gccattgccc aggctgacag
     1081 gctgacccag gagcctgaga gcatccgcaa gtggcgagag gagcagagga aacggctgca
     1141 agagctggat gctgcatcta aggtcacgga acaggaatgg cgggagaagg ccaagaagga
     1201 cctggaggag tggaaccagc gccagagtga acaagtagag aagaacaaga tcaacaaccg
     1261 ggcatccgag gaggctttcg tgaaggaatc caaggaggag accccaggca cagagtggga
     1321 gaaggtggcc cagctatgtg acttcaaccc caagagcagc aagcagtgca aagatgtgtc
     1381 ccgcctgcgc tcggtgctca tgtccctgaa gcagacgcca ctgtcccgct agggatccac
     1441 cggatctaga taactgatca taatcagcca taccacatt
//
