LOCUS       sequence-85815-e        2432 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane/Vesicles, Excitation: 433, Emission: 475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Markwardt ML, Kremers GJ, Kraft CA, Ray K, Cranfill PJ, Wilson KA, 
            Day RN, Wachter RM, Davidson MW, Rizzo MA
  TITLE     An improved cerulean fluorescent protein with enhanced brightness 
            and reduced reversible photoswitching.
  JOURNAL   PLoS One. 2011  . 6(3):e17896.
  PUBMED    21479270
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2011  . 6(3):e17896."
COMMENT     Sequence Label: mCerulean3-C-Src-7
FEATURES             Location/Qualifiers
     source          1..2432
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             53..94
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     regulatory      1667..1676
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1673..1675,1676..1678,1679..2392)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIK
                     ANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1718..1739)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1979..1998)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2326..2347
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tcagatccgc tagcgctacc ggactcagat ctcgagctca agcttcgaat tcatggggag
       61 cagcaagagc aagcccaagg accccagcca gcgccggcgc agcctggagc cacccgacag
      121 cacccaccac gggggattcc cagcctcgca gacccccaac aagacagcag cccccgacac
      181 gcaccgcacc cccagccgct cctttgggac cgtggccacc gagcccaagc tcttcggggg
      241 cttcaacact tctgacaccg tcacgtcgcc gcagcgtgcc ggggcactgg ctggcggcgt
      301 caccactttc gtggctctct atgactacga gtcccggact gaaacggact tgtccttcaa
      361 gaaaggagaa cgcctgcaga ttgtcaacaa cacggaaggt gactggtggc tggctcattc
      421 cctcactaca ggacagacgg gctacatccc cagtaactat gtcgcgccct cagactccat
      481 ccaggctgaa gagtggtact ttgggaagat cactcgtcgg gagtccgagc ggctgctgct
      541 caaccccgaa aacccccggg gaaccttctt ggtccgggag agcgagacga caaaaggtgc
      601 ctattgcctc tccgtttctg actttgacaa cgccaagggg ctcaatgtga agcactacaa
      661 gatccgcaag ctggacagcg gcggcttcta catcacctca cgcacacagt tcagcagcct
      721 gcagcagctg gtggcctact actccaaaca tgctgatggc ttgtgccacc gcctgaccaa
      781 cgtctgcccc acgtccaagc cccagaccca gggactcgcc aaggacgcgt gggaaatccc
      841 ccgggagtcg ctgcggctgg aggtgaagct ggggcagggc tgctttggag aggtctggat
      901 ggggacctgg aacggcacca ccagagtggc cataaagact ctgaagcccg gcaccatgtc
      961 cccggaggcc ttcctgcagg aagcccaagt gatgaagaag ctccggcatg agaagctggt
     1021 tcagctgtac gcagtggtgt cggaagagcc catctacatc gtcactgagt acatgagcaa
     1081 ggggagcctc ctggatttcc tgaagggaga gatgggcaag tacctgcggc tgccacagct
     1141 cgtcgatatg gctgctcaga ttgcatccgg catggcctat gtggagagga tgaactacgt
     1201 gcaccgagac ctgcgggcgg ccaacatcct ggtgggggag aacctggtgt gcaaggtggc
     1261 tgactttggg ctggcacgcc tcatcgagga caacgagtac acagcacggc aaggtgccaa
     1321 gttccccatc aagtggacag cccccgaggc agccctctat ggccggttca ccatcaagtc
     1381 ggatgtctgg tccttcggca tcctgctgac tgagctgacc accaagggcc gggtgccata
     1441 cccagggatg gtcaacaggg aggtgctgga ccaggtggag aggggctacc gcatgccctg
     1501 cccgcccgag tgccccgagt cgctgcatga cctcatgtgc cagtgctggc ggaaggaccc
     1561 tgaggagcgg cccacttttg agtacctgca ggccttcctg gaggactact tcacctcgac
     1621 agagccccag taccagcctg gagagaacct acgggatcca ccggtcgcca ccatggtgag
     1681 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     1741 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     1801 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     1861 caccctgagc tggggcgtgc agtgcttcgc ccgctacccc gaccacatga agcagcacga
     1921 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     1981 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     2041 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     2101 gtacaacgcc atccacggca acgtctatat caccgccgac aagcagaaga acggcatcaa
     2161 ggccaacttc ggcctcaact gcaacatcga ggacggcagc gtgcagctcg ccgaccacta
     2221 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     2281 cacccagtcc aagctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     2341 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt aaagcggccg
     2401 cgactctaga tcataatcag ccataccaca tt
//
