LOCUS       sequence-85816-e        1532 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Gap Junctions, Excitation: 433, Emission: 475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Markwardt ML, Kremers GJ, Kraft CA, Ray K, Cranfill PJ, Wilson KA, 
            Day RN, Wachter RM, Davidson MW, Rizzo MA
  TITLE     An improved cerulean fluorescent protein with enhanced brightness 
            and reduced reversible photoswitching.
  JOURNAL   PLoS One. 2011  . 6(3):e17896.
  PUBMED    21479270
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2011  . 6(3):e17896."
COMMENT     Sequence Label: mCerulean3-Cx26-7
FEATURES             Location/Qualifiers
     source          1..1532
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      791..800
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(797..799,800..802,803..1516)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIK
                     ANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(842..863)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1103..1122)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1450..1471
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gatccgctag cgctaccgga ctcagatctc gagctcaagc ttcgaattcg cggctgcacg
       61 ctcctcggga cacagtgcca accatccaga ggacaagatg gattggggca cactacagag
      121 catcctcggg ggtgtcaaca agcactccac cagcattggg aaaatctggc tcactgtcct
      181 cttcatcttc cgcatcatga tcctcgtggt ggccgcgaag gaggtgtggg gagatgagca
      241 agccgatttt gtttgcaaca ctctccagcc tggctgtaag aatgtgtgct acgaccacta
      301 cttccccatc tctcacatcc ggctctgggc tctgcagctg atcatggtgt ccacgccggc
      361 cctcctggta gctatgcacg tggcctaccg gagacacgaa aagaaacgga agttcatgaa
      421 gggagagata aagaacgagt ttaaggacat cgaagagatc aaaacccaga aggtccgtat
      481 cgaagggtcc ctgtggtgga cctacaccac cagcatcttc ttccgggtca tcttcgaagc
      541 tgtcttcatg tatgtctttt acatcatgta caatggcttc ttcatgcagc gtctggtgaa
      601 gtgtaacgcc tggccttgtc ccaatacagt ggactgcttc atttccaggc ccacagaaaa
      661 gactgtcttc acggtgttca tgatctctgt gtctggaatt tgcatcctgc taaacatcac
      721 agagctgtgc tatctgttca ttaggtattg ctcagggaag tccaaaagac cagtcgcgga
      781 tccaccggtc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
      841 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
      901 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
      961 cgtgccctgg cccaccctcg tgaccaccct gagctggggc gtgcagtgct tcgcccgcta
     1021 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     1081 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     1141 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     1201 caacatcctg gggcacaagc tggagtacaa cgccatccac ggcaacgtct atatcaccgc
     1261 cgacaagcag aagaacggca tcaaggccaa cttcggcctc aactgcaaca tcgaggacgg
     1321 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     1381 gctgcccgac aaccactacc tgagcaccca gtccaagctg agcaaagacc ccaacgagaa
     1441 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     1501 cgagctgtac aagtaaagcg gccgcgactt ct
//
