LOCUS       sequence-85817-e        2167 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Gap Junctions, Excitation: 433, Emission: 475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Markwardt ML, Kremers GJ, Kraft CA, Ray K, Cranfill PJ, Wilson KA, 
            Day RN, Wachter RM, Davidson MW, Rizzo MA
  TITLE     An improved cerulean fluorescent protein with enhanced brightness 
            and reduced reversible photoswitching.
  JOURNAL   PLoS One. 2011  . 6(3):e17896.
  PUBMED    21479270
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2011  . 6(3):e17896."
COMMENT     Sequence Label: mCerulean3-Cx43-7
FEATURES             Location/Qualifiers
     source          1..2167
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1401..1410
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1407..1409,1410..1412,1413..2126)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIK
                     ANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1452..1473)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1713..1732)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2060..2081
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 agatccgcta gcgctaccgg actcagatct cgagctcaag cttcgaattc ggcagcactt
       61 ttctttcatt ggggggaagg cgtgaggaaa gtaccaaaca gcagcagact tttaaacttt
      121 aaacagacag gtctgagagc ctgaactctc atttttcctt tgacttcagc ctccaaggag
      181 ttccaccaac tttggcgcgc cggcttcact ttcattaagt gaaagagagg tgcccagaca
      241 tgggtgactg gagtgccttg gggaagcttc tggacaaggt ccaagcctac tccaccgctg
      301 gagggaaggt gtggctgtca gtgctcttca tattcagaat cctgctcctg gggacagctg
      361 ttgagtcagc ttggggtgat gaacagtctg cctttcgctg taacactcaa caacctggct
      421 gcgaaaacgt ctgctatgac aagtccttcc ccatctctca cgtgcgcttc tgggtccttc
      481 agatcatatt cgtgtctgtg cccacactcc tgtacttggc ccatgtgttc tatgtgatga
      541 ggaaggaaga gaagctaaac aagaaagaag aggagctcaa agtggcccag actgacgggg
      601 tcaacgtgga gatgcacctg aagcagattg aaatcaagaa gttcaagtac gggattgaag
      661 agcacggcaa ggtgaaaatg aggggcggct tgctgagaac ctacatcatc agcatcctct
      721 tcaagtctgt cttcgaggtg gccttcctgc tcatccagtg gtacatctat gggttcagct
      781 tgagcgcggt ctacacctgc aagagagatc cctgccccca ccaggtagac tgcttcctct
      841 cacgtcccac ggagaaaacc atcttcatca tcttcatgct ggtggtgtcc ttggtgtctc
      901 tcgctttgaa catcattgag ctcttctacg tcttcttcaa aggcgttaag gatcgcgtga
      961 agggaagaag cgatccttac cacgccacca ctggcccact gagcccatca aaagactgcg
     1021 gatctccaaa atacgcctac ttcaatggct gctcctcacc aacggctcca ctctcgccta
     1081 tgtctcctcc tgggtacaag ctggttactg gtgacagaaa caattcctcg tgccgcaatt
     1141 acaacaagca agctagcgag caaaactggg cgaactacag cgcagagcaa aatcgcatgg
     1201 ggcaggccgg aagcaccatc tccaactcgc acgcccagcc gttcgatttc cccgacgaca
     1261 accagaatgc caaaaaagtt gctgctggac atgaactcca gccattagcc atcgtggacc
     1321 aacgaccttc cagcagagcc agcagccgcg ccagcagcag gcctcggcct gatgacctgg
     1381 agattgcgga tccaccggtc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg
     1441 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
     1501 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
     1561 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gagctggggc gtgcagtgct
     1621 tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     1681 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     1741 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     1801 aggaggacgg caacatcctg gggcacaagc tggagtacaa cgccatccac ggcaacgtct
     1861 atatcaccgc cgacaagcag aagaacggca tcaaggccaa cttcggcctc aactgcaaca
     1921 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     1981 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccaagctg agcaaagacc
     2041 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     2101 tcggcatgga cgagctgtac aagtaaagcg gccgcgactc tagatcataa tcagccatac
     2161 cacattt
//
