LOCUS       sequence-85819-e        1560 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane, Excitation: 433, Emission: 475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Markwardt ML, Kremers GJ, Kraft CA, Ray K, Cranfill PJ, Wilson KA, 
            Day RN, Wachter RM, Davidson MW, Rizzo MA
  TITLE     An improved cerulean fluorescent protein with enhanced brightness 
            and reduced reversible photoswitching.
  JOURNAL   PLoS One. 2011  . 6(3):e17896.
  PUBMED    21479270
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2011  . 6(3):e17896."
COMMENT     Sequence Label: mCerulean3-Dectin1A-C-10
FEATURES             Location/Qualifiers
     source          1..1560
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      13..22
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(19..21,22..24,25..735)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIK
                     ANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(64..85)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(325..344)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     672..693
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gcgctaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
       61 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
      121 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
      181 cccgtgccct ggcccaccct cgtgaccacc ctgagctggg gcgtgcagtg cttcgcccgc
      241 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
      301 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
      361 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
      421 ggcaacatcc tggggcacaa gctggagtac aacgccatcc acggcaacgt ctatatcacc
      481 gccgacaagc agaagaacgg catcaaggcc aacttcggcc tcaactgcaa catcgaggac
      541 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
      601 ctgctgcccg acaaccacta cctgagcacc cagtccaagc tgagcaaaga ccccaacgag
      661 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
      721 gacgagctgt acaagtccgg actcagatct ggcagcagtg gaggtgaata tcatcctgat
      781 ttagaaaatt tggatgaaga tggatatact caattacact tcgactctca aagcaatacc
      841 aggatagctg ttgtttcaga gaaaggatcg tgtgctgcat ctcctccttg gcgcctcatt
      901 gctgtaattt tgggaatcct atgcttggta atactggtga tagctgtggt cctgggtacc
      961 atggctattt ggagatccaa ttcaggaagc aacacattgg agaatggcta ctttctatca
     1021 agaaataaag agaaccacag tcaacccaca caatcatctt tagaagacag tgtgactcct
     1081 accaaagctg tcaaaaccac aggggttctt tccagccctt gtcctcctaa ttggattata
     1141 tatgagaaga gctgttatct attcagcatg tcactaaatt cctgggatgg aagtaaaaga
     1201 caatgctggc aactgggctc taatctccta aagatagaca gctcaaatga attgggattt
     1261 atagtaaaac aagtgtcttc ccaacctgat aattcatttt ggataggcct ttctcggccc
     1321 cagactgaag taccatggct ctgggaggat ggatcaacat tctcttctaa cttatttcag
     1381 atcagaacca cagctaccca agaaaaccca tctccaaatt gtgtatggat tcacgtgtca
     1441 gtcatttatg accaactgtg tagtgtgccc tcatatagta tttgtgagaa gaagttttca
     1501 atgtaagaat tctgcagtcg acggtaccgc gggcccggga tccaccggat ctagataact
//
