LOCUS       sequence-85820-e        1556 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane, Excitation: 433, Emission: 475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Markwardt ML, Kremers GJ, Kraft CA, Ray K, Cranfill PJ, Wilson KA, 
            Day RN, Wachter RM, Davidson MW, Rizzo MA
  TITLE     An improved cerulean fluorescent protein with enhanced brightness 
            and reduced reversible photoswitching.
  JOURNAL   PLoS One. 2011  . 6(3):e17896.
  PUBMED    21479270
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2011  . 6(3):e17896."
COMMENT     Sequence Label: mCerulean3-Dectin1A-N-10
FEATURES             Location/Qualifiers
     source          1..1556
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      790..799
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(796..798,799..801,802..1515)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIK
                     ANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(841..862)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1102..1121)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1449..1470
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcgc caccatggaa tatcatcctg atttagaaaa tttggatgaa
       61 gatggatata ctcaattaca cttcgactct caaagcaata ccaggatagc tgttgtttca
      121 gagaaaggat cgtgtgctgc atctcctcct tggcgcctca ttgctgtaat tttgggaatc
      181 ctatgcttgg taatactggt gatagctgtg gtcctgggta ccatggctat ttggagatcc
      241 aattcaggaa gcaacacatt ggagaatggc tactttctat caagaaataa agagaaccac
      301 agtcaaccca cacaatcatc tttagaagac agtgtgactc ctaccaaagc tgtcaaaacc
      361 acaggggttc tttccagccc ttgtcctcct aattggatta tatatgagaa gagctgttat
      421 ctattcagca tgtcactaaa ttcctgggat ggaagtaaaa gacaatgctg gcaactgggc
      481 tctaatctcc taaagataga cagctcaaat gaattgggat ttatagtaaa acaagtgtct
      541 tcccaacctg ataattcatt ttggataggc ctttctcggc cccagactga agtaccatgg
      601 ctctgggagg atggatcaac attctcttct aacttatttc agatcagaac cacagctacc
      661 caagaaaacc catctccaaa ttgtgtatgg attcacgtgt cagtcattta tgaccaactg
      721 tgtagtgtgc cctcatatag tatttgtgag aagaagtttt caatgagctc cggaggggat
      781 ccaccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
      841 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
      901 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
      961 gtgccctggc ccaccctcgt gaccaccctg agctggggcg tgcagtgctt cgcccgctac
     1021 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     1081 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     1141 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     1201 aacatcctgg ggcacaagct ggagtacaac gccatccacg gcaacgtcta tatcaccgcc
     1261 gacaagcaga agaacggcat caaggccaac ttcggcctca actgcaacat cgaggacggc
     1321 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     1381 ctgcccgaca accactacct gagcacccag tccaagctga gcaaagaccc caacgagaag
     1441 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     1501 gagctgtaca agtaaagcgg ccgcgactct agatcataat cagccatacc acattt
//
