LOCUS       sequence-85821-e        1657 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: MT End Binding Protein, Excitation: 433, Emission: 
            475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Markwardt ML, Kremers GJ, Kraft CA, Ray K, Cranfill PJ, Wilson KA, 
            Day RN, Wachter RM, Davidson MW, Rizzo MA
  TITLE     An improved cerulean fluorescent protein with enhanced brightness 
            and reduced reversible photoswitching.
  JOURNAL   PLoS One. 2011  . 6(3):e17896.
  PUBMED    21479270
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2011  . 6(3):e17896."
COMMENT     Sequence Label: mCerulean3-EB1-C-18
FEATURES             Location/Qualifiers
     source          1..1657
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      30..39
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(36..38,39..41,42..752)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIK
                     ANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(81..102)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(342..361)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     689..710
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tgacgtcaga tccgctagcg ctaccggtcg ccaccatggt gagcaagggc gaggagctgt
       61 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
      121 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
      181 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg agctggggcg
      241 tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
      301 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
      361 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
      421 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac gccatccacg
      481 gcaacgtcta tatcaccgcc gacaagcaga agaacggcat caaggccaac ttcggcctca
      541 actgcaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
      601 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccaagctga
      661 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
      721 ggatcactct cggcatggac gagctgtaca agtccggact cagatctggc agcggtggag
      781 gcagcgcatc cggcggaagc ggaagcgcag tgaacgtata ctcaacgtca gtgaccagtg
      841 ataacctaag tcgacatgac atgctggcct ggatcaatga gtctctgcag ttgaatctga
      901 caaagatcga acagttgtgc tcaggggctg cgtattgtca gtttatggac atgctgttcc
      961 ctggctccat tgccttgaag aaagtgaaat tccaagctaa gctagaacac gagtacatcc
     1021 agaacttcaa aatattacaa gcaggtttta agagaatggg tgttgacaaa ataattcctg
     1081 tggacaaatt agtaaaagga aagtttcagg acaattttga attcgttcag tggttcaaga
     1141 agttcttcga tgcaaactat gatggaaaag actatgaccc tgtggctgct agacaaggtc
     1201 aagaaactgc agtggctcct tcccttgttg ctccagctct gaataaaccg aagaaacctc
     1261 tcacttctag cagtgcagct ccccagaggc ccatctcaac acagagaacc gctgcggctc
     1321 ctaaggctgg ccctggtgtg gtgcgaaaga accctggtgt gggcaacgga gacgacgagg
     1381 cagctgagtt gatgcagcag gtcaacgtat tgaaacttac tgttgaagac ttggagaaag
     1441 agagggattt ctacttcgga aagctacgga acattgaatt gatttgccag gagaacgagg
     1501 gggaaaacga ccctgtattg cagaggattg tagacattct gtatgccaca gatgaaggct
     1561 ttgtgatacc tgatgaaggg ggcccacagg aggagcaaga agagtattaa ggatccaccg
     1621 gatctagata actgatcata atcagccata ccacatt
//
