LOCUS       sequence-85822-e        1696 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: MT End Binding Protein, Excitation: 433, Emission: 
            475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Markwardt ML, Kremers GJ, Kraft CA, Ray K, Cranfill PJ, Wilson KA, 
            Day RN, Wachter RM, Davidson MW, Rizzo MA
  TITLE     An improved cerulean fluorescent protein with enhanced brightness 
            and reduced reversible photoswitching.
  JOURNAL   PLoS One. 2011  . 6(3):e17896.
  PUBMED    21479270
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2011  . 6(3):e17896."
COMMENT     Sequence Label: mCerulean3-EB3-7
FEATURES             Location/Qualifiers
     source          1..1696
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      67..76
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      931..940
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(937..939,940..942,943..1656)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIK
                     ANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(982..1003)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1243..1262)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1590..1611
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgct accggactca gatctcgagc tcaagcttcg aattctgcag
       61 tcgactgcca ccatggccgt caatgtgtac tccacatctg tgaccagtga aaatctgagt
      121 cgccatgata tgcttgcatg ggtcaacgac tccctgcacc tcaactatac caagatagaa
      181 cagctttgtt caggggcagc ctactgccag ttcatggaca tgctcttccc cggctgtgtg
      241 cacttgagga aagtgaagtt ccaggccaaa ctagagcatg aatacatcca caacttcaag
      301 gtgctgcaag cagctttcaa gaagatgggt gttgacaaaa tcattcctgt agagaaatta
      361 gtgaaaggaa aattccaaga taattttgag tttattcagt ggtttaagaa attctttgac
      421 gcaaactatg atggaaagga ttacaaccct ctgctggcgc ggcagggcca ggacgtagcg
      481 ccacctccta acccaggtga tcagatcttc aacaaatcca agaaactcat tggcacagca
      541 gttccacaga ggacgtcccc cacaggccca aaaaacatgc agacctctgg ccggctgagc
      601 aatgtggccc ccccctgcat tctccggaag aatcctccat cagcccgaaa tggcggccat
      661 gagactgatg cccaaattct tgaactcaac caacagctgg tggacttgaa gctgacagtg
      721 gatgggctgg agaaggaacg tgacttctac ttcagcaaac ttcgtgacat cgagctcatc
      781 tgccaggagc atgaaagtga aaacagccct gttatctcag gcatcattgg catcctctat
      841 gccacagagg aaggattcgc accccctgag gacgatgaga ttgaagagca tcaacaagaa
      901 gaccaggacg agtacctgga tccaccggtc gccaccatgg tgagcaaggg cgaggagctg
      961 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     1021 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     1081 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gagctggggc
     1141 gtgcagtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     1201 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     1261 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     1321 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa cgccatccac
     1381 ggcaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggccaa cttcggcctc
     1441 aactgcaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     1501 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccaagctg
     1561 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     1621 gggatcactc tcggcatgga cgagctgtac aagtaaagcg gccgcgactc tagatcataa
     1681 tcagccatac cacatt
//
