LOCUS       sequence-85823-e        1700 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: MT End Binding Protein, Excitation: 433, Emission: 
            475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Markwardt ML, Kremers GJ, Kraft CA, Ray K, Cranfill PJ, Wilson KA, 
            Day RN, Wachter RM, Davidson MW, Rizzo MA
  TITLE     An improved cerulean fluorescent protein with enhanced brightness 
            and reduced reversible photoswitching.
  JOURNAL   PLoS One. 2011  . 6(3):e17896.
  PUBMED    21479270
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2011  . 6(3):e17896."
COMMENT     Sequence Label: mCerulean3-EB3-C-20
FEATURES             Location/Qualifiers
     source          1..1700
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      27..36
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(33..35,36..38,39..749)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIK
                     ANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(78..99)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(339..358)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     686..707
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cgtcagatcc gctagcgcta ccggtcgcca ccatggtgag caagggcgag gagctgttca
       61 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
      121 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
      181 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgagc tggggcgtgc
      241 agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
      301 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
      361 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
      421 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaacgcc atccacggca
      481 acgtctatat caccgccgac aagcagaaga acggcatcaa ggccaacttc ggcctcaact
      541 gcaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
      601 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc aagctgagca
      661 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
      721 tcactctcgg catggacgag ctgtacaagt ccggactcag atctggcagc ggtggaggca
      781 gcgcatccgg cggaagcgga agcgggggag ccgtcaatgt gtactccaca tctgtgacca
      841 gtgaaaatct gagtcgccat gatatgcttg catgggtcaa cgactccctg cacctcaact
      901 ataccaagat agaacagctt tgttcagggg cagcctactg ccagttcatg gacatgctct
      961 tccccggctg tgtgcacttg aggaaagtga agttccaggc caaactagag catgaataca
     1021 tccacaactt caaggtgctg caagcagctt tcaagaagat gggtgttgac aaaatcattc
     1081 ctgtagagaa attagtgaaa ggaaaattcc aagataattt tgagtttatt cagtggttta
     1141 agaaattctt tgacgcaaac tatgatggaa aggattacaa ccctctgctg gcgcggcagg
     1201 gccaggacgt agcgccacct cctaacccag gtgatcagat attcaacaaa tccaagaaac
     1261 tcattggcac agcagttcca cagaggacgt cccccacagg cccaaaaaac atgcagacct
     1321 ctggccggct gagcaatgtg gcccccccct gcattctccg gaagaatcct ccatcagccc
     1381 gaaatggcgg ccatgagact gatgcccaaa ttcttgaact caaccaacag ctggtggact
     1441 tgaagctgac agtggatggg ctggagaagg aacgtgactt ctacttcagc aaacttcgtg
     1501 acatcgagct catctgccag gagcatgaaa gtgaaaacag ccctgttatc tcaggcatca
     1561 ttggcatcct ctatgccaca gaggaaggat tcgcaccccc tgaggacgat gagattgaag
     1621 agcatcaaca agaagaccag gacgagtact aaggatccac cggatctaga taactgatca
     1681 taatcagcca taccacattt
//
