LOCUS       sequence-85825-e        1780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleoli, Excitation: 433, Emission: 475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Markwardt ML, Kremers GJ, Kraft CA, Ray K, Cranfill PJ, Wilson KA, 
            Day RN, Wachter RM, Davidson MW, Rizzo MA
  TITLE     An improved cerulean fluorescent protein with enhanced brightness 
            and reduced reversible photoswitching.
  JOURNAL   PLoS One. 2011  . 6(3):e17896.
  PUBMED    21479270
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2011  . 6(3):e17896."
COMMENT     Sequence Label: mCerulean3-Fibrillarin-7
FEATURES             Location/Qualifiers
     source          1..1780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      23..32
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(29..31,32..34,35..745)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIK
                     ANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(74..95)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(335..354)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     682..703
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(1541..1552)
                     /codon_start=1
                     /gene="cspA"
                     /product="translation enhancing element for E. coli (Qing 
                     et al., 2004)"
                     /label=TEE
                     /translation="NHKV"
ORIGIN
        1 gaatccgcta gcgctaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg
       61 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
      121 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
      181 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgagctggg gcgtgcagtg
      241 cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
      301 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
      361 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
      421 caaggaggac ggcaacatcc tggggcacaa gctggagtac aacgccatcc acggcaacgt
      481 ctatatcacc gccgacaagc agaagaacgg catcaaggcc aacttcggcc tcaactgcaa
      541 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
      601 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccaagc tgagcaaaga
      661 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
      721 tctcggcatg gacgagctgt acaagtccgg actcagatct cgagccatga agccaggatt
      781 cagtccccgt gggggtggct ttggcggccg agggggcttt ggtgaccgtg gtggtcgtgg
      841 aggccgaggg ggctttggcg ggggccgagg ccaaggcgga ggctttagag gtcgtggacg
      901 aggaggaggt ggaggcggcg gcggcggtgg aggaggagga agaggtggtg gaggcttcca
      961 ttttggtggc aaccggggtc gtggtcgggg aggaaaaaga ggaaaccagt cggggaagaa
     1021 tgtgatggtg gagccgcatc ggcatgaggg tgtcttcatt tgtcggggaa aggaagatgc
     1081 actggtcacc aagaacctgg tccctgggga atcagtttat ggagagaaga gagtctcgat
     1141 ttcggaagga gatgacaaaa ttgagtaccg agcctggaac cccttccgct ccaagcttgc
     1201 agcagcaatc ctgggtggtg tggaccagat ccacatcaaa ccgggggcta aggttctcta
     1261 cctcggggct gcctcgggca ccacggtctc ccatgtctct gacatcgttg gtccggatgg
     1321 tctagtctat gcagtcgagt tctcccaccg ctctggccgt gacctcatta acttggccaa
     1381 gaagaggacc aacatcattc ctgtgatcga ggatgctcga cacccacaca aataccgcat
     1441 gctcatcgca atggtggatg tgatctttgc tgatgtggcc cagccagacc agacccggat
     1501 tgtggccctg aatgcccaca ccttcctgcg taatggagga cactttgtga tttccattaa
     1561 ggccaactgc attgactcca cagcctcagc cgaggccgtg tttgcctccg aagtgaaaaa
     1621 gatgcaacag gagaacatga agccgcagga gcagttgacc cttgagccat atgaaagaga
     1681 ccatgccgtg gtcgtgggag tgtacaggcc accccccaag gtgaagaact gaggatccac
     1741 cggatctaga taactgatca taatcagcca taccacattt
//
