LOCUS       sequence-85830-e        2555 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nuclear Envelope, Excitation: 433, Emission: 475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Markwardt ML, Kremers GJ, Kraft CA, Ray K, Cranfill PJ, Wilson KA, 
            Day RN, Wachter RM, Davidson MW, Rizzo MA
  TITLE     An improved cerulean fluorescent protein with enhanced brightness 
            and reduced reversible photoswitching.
  JOURNAL   PLoS One. 2011  . 6(3):e17896.
  PUBMED    21479270
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2011  . 6(3):e17896."
COMMENT     Sequence Label: mCerulean3-LaminB1-10
FEATURES             Location/Qualifiers
     source          1..2555
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      14..23
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(20..22,23..25,26..736)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIK
                     ANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(65..86)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(326..345)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     673..694
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gcgcttaccg gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
       61 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
      121 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
      181 gcccgtgccc tggcccaccc tcgtgaccac cctgagctgg ggcgtgcagt gcttcgcccg
      241 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
      301 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
      361 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
      421 cggcaacatc ctggggcaca agctggagta caacgccatc cacggcaacg tctatatcac
      481 cgccgacaag cagaagaacg gcatcaaggc caacttcggc ctcaactgca acatcgagga
      541 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
      601 gctgctgccc gacaaccact acctgagcac ccagtccaag ctgagcaaag accccaacga
      661 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
      721 ggacgagctg tacaagtccg gactcagatc tcgagctcaa gcttcgatgg cgactgcgac
      781 ccccgtgccg ccgcggatgg gcagccgcgc tggcggcccc accacgccgc tgagccccac
      841 gcgcctgtcg cggctccagg agaaggagga gctgcgcgag ctcaatgacc ggctggcggt
      901 gtacatcgac aaggtgcgca gcctggagac ggagaacagc gcgctgcagc tgcaggtgac
      961 ggagcgcgag gaggtgcgcg gccgtgagct caccggcctc aaggcgctct acgagaccga
     1021 gctggccgac gcgcgacgcg cgctcgacga cacggcccgc gagcgcgcca agctgcagat
     1081 cgagctgggc aagtgcaagg cggaacacga ccagctgctc ctcaactatg ctaagaagga
     1141 atctgatctt aatggcgccc agatcaagct tcgagaatat gaagcagcac tcaattcgaa
     1201 agatgcagct cttgctactg cacttggtga caaaaaaagt ttagagggag atttggagga
     1261 tctgaaggat cagattgccc agttggaagc ctccttagct gcagccaaaa aacagttagc
     1321 agatgaaact ttacttaaag tagatttgga gaatcgttgt cagagcctta ctgaggactt
     1381 ggagtttcgc aaaagcatgt atgaagagga gattaacgag accagaagga agcatgaaac
     1441 gcgcttggta gaggtggatt ctgggcgtca aattgagtat gagtacaagc tggcgcaagc
     1501 ccttcatgag atgagagagc aacatgatgc ccaagtgagg ctgtataagg aggagctgga
     1561 gcagacttac catgccaaac ttgagaatgc cagactgtca tcagagatga atacttctac
     1621 tgtcaacagt gccagggaag aactgatgga aagccgcatg agaattgaga gcctttcatc
     1681 ccagctttct aatctacaga aagagtctag agcatgtttg gaaaggattc aagaattaga
     1741 ggacttgctt gctaaagaaa aagacaactc tcgtcgcatg ctgacagaca aagagagaga
     1801 gatggcggaa ataagggatc aaatgcagca acagctgaat gactatgaac agcttcttga
     1861 tgtaaagtta gccctggaca tggaaatcag tgcttacagg aaactcttag aaggcgaaga
     1921 agagaggttg aagctgtctc caagcccttc ttcccgtgtg acagtatccc gagcatcctc
     1981 aagtcgtagt gtacgtacaa ctagaggaaa gcggaagagg gttgatgtgg aagaatcaga
     2041 ggcgagtagt agtgttagca tctctcattc cgcctcagcc actggaaatg tttgcatcga
     2101 agaaattgat gttgatggga aatttatccg cttgaagaac acttctgaac aggatcaacc
     2161 aatgggaggc tgggagatga tcagaaaaat tggagacaca tcagtcagtt ataaatatac
     2221 ctcaagatat gtgctgaagg caggccagac tgttacaatt tgggctgcaa acgctggtgt
     2281 cacagccagc cccccaactg acctcatctg gaagaaccag aactcgtggg gcactggcga
     2341 agatgtgaag gttatattga aaaattctca gggagaggag gttgctcaaa gaagtacagt
     2401 ctttaaaaca accatacctg aagaagagga ggaggaggaa gaagcagctg gagtggttgt
     2461 tgaggaagaa cttttccacc agcagggaac cccaagagca tccaatagaa gctgtgcaat
     2521 tatgtaggga tccaccggat ctagataact gattc
//
