LOCUS       sequence-85836-e        1875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Microtubules, Excitation: 433, Emission: 475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Markwardt ML, Kremers GJ, Kraft CA, Ray K, Cranfill PJ, Wilson KA, 
            Day RN, Wachter RM, Davidson MW, Rizzo MA
  TITLE     An improved cerulean fluorescent protein with enhanced brightness 
            and reduced reversible photoswitching.
  JOURNAL   PLoS One. 2011  . 6(3):e17896.
  PUBMED    21479270
REFERENCE   2  (bases 1 to 1875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2011  . 6(3):e17896."
COMMENT     Sequence Label: mCerulean3-MAPTau-C-10
FEATURES             Location/Qualifiers
     source          1..1875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      20..29
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(26..28,29..31,32..742)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIK
                     ANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(71..92)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(332..351)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     679..700
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tccgctagcg ctaccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt
       61 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
      121 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
      181 caagctgccc gtgccctggc ccaccctcgt gaccaccctg agctggggcg tgcagtgctt
      241 cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
      301 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
      361 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
      421 ggaggacggc aacatcctgg ggcacaagct ggagtacaac gccatccacg gcaacgtcta
      481 tatcaccgcc gacaagcaga agaacggcat caaggccaac ttcggcctca actgcaacat
      541 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
      601 ccccgtgctg ctgcccgaca accactacct gagcacccag tccaagctga gcaaagaccc
      661 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
      721 cggcatggac gagctgtaca agtccggaag cggcgggtcc gggagcggaa gcgctgagcc
      781 ccgccaggag ttcgaagtga tggaagatca cgctgggacg tacgggttgg gggacaggaa
      841 agatcagggg ggctacacca tgcaccaaga ccaagagggt gacacggacg ctggcctgaa
      901 agctgaagaa gcaggcattg gagacacccc cagcctggaa gacgaagctg ctggtcacgt
      961 gacccaagct cgcatggtca gtaaaagcaa agacgggact ggaagcgatg acaaaaaagc
     1021 caagggggct gatggtaaaa cgaagatcgc cacaccgcgg ggagcagccc ctccaggcca
     1081 gaagggccag gccaacgcca ccaggattcc agcaaaaacc ccgcccgctc caaagacacc
     1141 acccagctct ggtgaacctc caaaatcagg ggatcgcagc ggctacagca gccccggctc
     1201 cccaggcact cccggcagcc gctcccgcac cccgtccctt ccaaccccac ccacccggga
     1261 gcccaagaag gtggcagtgg tccgtactcc acccaagtcg ccgtcttccg ccaagagccg
     1321 cctgcagaca gcccccgtgc ccatgccaga cctgaagaat gtcaagtcca agatcggctc
     1381 cactgagaac ctgaagcacc agccgggagg cgggaaggtg caaatagtct acaaaccagt
     1441 tgacctgagc aaggtgacct ccaagtgtgg ctcattaggc aacatccatc ataaaccagg
     1501 aggtggccag gtggaagtaa aatctgagaa gcttgacttc aaggacagag tccagtcgaa
     1561 gattgggtcc ctggacaata tcacccacgt ccctggcgga ggaaataaaa agattgaaac
     1621 ccacaagctg accttccgcg agaacgccaa agccaagaca gaccacgggg cggagatcgt
     1681 gtacaagtcg ccagtggtgt ctggggacac gtctccacgg catctcagca atgtctcctc
     1741 caccggcagc atcgacatgg tagactcgcc ccagctcgcc acgctagctg acgaggtgtc
     1801 tgcctccctg gccaagcagg gtttgtgagg atccaccgga tctagataac tgatcataat
     1861 cagccatacc acatt
//
