LOCUS       sequence-85839-e        2334 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 433, Emission: 475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Markwardt ML, Kremers GJ, Kraft CA, Ray K, Cranfill PJ, Wilson KA, 
            Day RN, Wachter RM, Davidson MW, Rizzo MA
  TITLE     An improved cerulean fluorescent protein with enhanced brightness 
            and reduced reversible photoswitching.
  JOURNAL   PLoS One. 2011  . 6(3):e17896.
  PUBMED    21479270
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2011  . 6(3):e17896."
COMMENT     Sequence Label: mCerulean3-Myotilin-C-14
FEATURES             Location/Qualifiers
     source          1..2334
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(34..36,37..39,40..750)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIK
                     ANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(79..100)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(340..359)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     687..708
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcga ggagctgttc
       61 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
      121 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
      181 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgag ctggggcgtg
      241 cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
      301 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
      361 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
      421 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacgc catccacggc
      481 aacgtctata tcaccgccga caagcagaag aacggcatca aggccaactt cggcctcaac
      541 tgcaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
      601 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc caagctgagc
      661 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
      721 atcactctcg gcatggacga gctgtacaag tccggaagtg ctgggagcgc aagcggaagc
      781 ggaggctccg gttttaacta cgaacgtcca aaacacttca tccagtccca aaacccatgt
      841 ggctccagat tgcagcctcc tggaccagaa acctccagct tctctagcca gaccaaacag
      901 tcttccatta tcatccagcc ccgccagtgt acagagcaaa gattttctgc ctcctcaaca
      961 ctgagctctc acatcaccat gtcctcctct gctttccctg cttctcccca gcagcatgct
     1021 ggctccaacc caggccaaag ggttacaacc acctataacc agtccccagc cagcttcctc
     1081 agctccatat taccatcaca gcctgattac aatagcagta aaatcccttc cgctatggat
     1141 tccaactatc aacagtcctc agctggccaa cctataaatg caaagccatc ccaaactgca
     1201 aatgctaagc ccataccaag aactcctgat catgaaatac aaggatcaaa agaagctttg
     1261 attcaagatt tggaaagaaa gctgaaatgc aaggacaccc ttcttcataa tggaaatcaa
     1321 cgtctaacat atgaagagaa gatggctcgc agattgctag gaccacagaa tgcagctgct
     1381 gtgtttcaag ctcaggatga cagtggtgca caagactcgc agcaacacaa ctcagaacat
     1441 gcgcgactgc aagttcctac atcacaagta agaagtagat caacctcaag gggagatgtg
     1501 aatgatcagg atgcaatcca ggagaaattt tacccaccac gtttcattca agtgccagag
     1561 aacatgtcga ttgatgaagg aagattctgc agaatggact tcaaagtgag tggactgcca
     1621 gctcctgatg tgtcatggta tctaaatgga agaacagttc aatcagatga tttgcacaaa
     1681 atgatagtgt ctgagaaggg tcttcattca ctcatctttg aagtagtcag agcttcagat
     1741 gcaggggctt atgcatgtgt tgccaagaat agagcaggag aagccacctt cactgtgcag
     1801 ctggatgtcc ttgcaaaaga acataaaaga gcaccaatgt ttatctacaa accacagagc
     1861 aaaaaagttt tagagggaga ttcagtgaaa ctagaatgcc agatctcggc tatacctcca
     1921 ccaaagcttt tctggaaaag aaataatgaa atggtacaat tcaacactga ccgaataagc
     1981 ttatatcaag ataacactgg aagagttact ttactgataa aagatgtaaa caagaaagat
     2041 gctgggtggt atactgtgtc agcagttaat gaagctggag tgactacatg taacacaaga
     2101 ttagacgtta cggcacgtcc aaaccaaact cttccagctc ctaagcagtt acgggttcga
     2161 ccaacattca gcaaatattt agcacttaat gggaaaggtt tgaatgtaaa acaagctttt
     2221 aacccagaag gagaatttca gcgtttggca gctcaatctg gactctatga aagtgaagaa
     2281 ctttaaggat ccaccggatc tagataactg atcataatca gccataccac attt
//
