LOCUS       sequence-85840-e        2838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 433, Emission: 475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Markwardt ML, Kremers GJ, Kraft CA, Ray K, Cranfill PJ, Wilson KA, 
            Day RN, Wachter RM, Davidson MW, Rizzo MA
  TITLE     An improved cerulean fluorescent protein with enhanced brightness 
            and reduced reversible photoswitching.
  JOURNAL   PLoS One. 2011  . 6(3):e17896.
  PUBMED    21479270
REFERENCE   2  (bases 1 to 2838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2011  . 6(3):e17896."
COMMENT     Sequence Label: mCerulean3-Palladin-C-7
FEATURES             Location/Qualifiers
     source          1..2838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      27..36
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(33..35,36..38,39..749)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIK
                     ANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(78..99)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(339..358)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     686..707
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gtcagaatcc gctagcgcta ccggtcgcca ccatggtgag caagggcgag gagctgttca
       61 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
      121 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
      181 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgagc tggggcgtgc
      241 agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
      301 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
      361 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
      421 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaacgcc atccacggca
      481 acgtctatat caccgccgac aagcagaaga acggcatcaa ggccaacttc ggcctcaact
      541 gcaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
      601 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc aagctgagca
      661 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
      721 tcactctcgg catggacgag ctgtacaagt ccggactcag atctcgagtg agcgctctgg
      781 cctcccgcgc taccgccatg cagtcctccg gctccttcaa ctacgcgcgc cctaagcagt
      841 tcatcgcggc gcagaacctg ggtcccgcat cggggctgcc cacgccaacc tccagcccca
      901 gctcctccag cttgccgtca ccattgtccc ccacgcccag gcccttcggc cgcgcgcccg
      961 ggccgccctt cgtggaaccc gaggccatgt ggggcccgtc ctcgccctcg ccgccaccgc
     1021 cgccacctcc ggtcttcagc ccctctgcag cctacccggt gcccgacgtg ttcccactgc
     1081 caccgccgcc gccaccgcta cctagctcca cctcacactg cgcctcgccc gcccgcttcg
     1141 gccccagcca gacgcccgca gccttcctca gcgccctgct gccctctcag ccaccgcctg
     1201 tcgctgtcaa cgccctgggg ctgcccaagg gagtcacccc cgcggggttc ccaaagaagt
     1261 ccagtagaac tgctagaatt gcctctgatg aggagattca aggcacaaag gatgctgtca
     1321 tccaagacct ggaacggaag cttcgcttca aggaggacct tctgaacaat ggccaaccga
     1381 ggctaaccta tgaggaaaga atggctcgcc gcctgcttgg agccgacagc gcaaacgtct
     1441 tcaacatcca ggagccagag gaaacggcag ccaatcagga gtacaaagtc tctagctgcg
     1501 agcagaggct gattagcgag attgaataca ggctggagcg ctcccctgtg gatgagtcgg
     1561 gagacgaggt gcaggatcca gatgtgcctg tggagaacgc aacagctccc ttctttgaga
     1621 tgaagctgaa acactacaag atctttgagg ggatgccggt gactttcacg tgtcgagtgg
     1681 ctgggaatcc aaagccaaag atctattggt ttaaagatgg gaagcagatt tctccgaaga
     1741 gcgatcacta caccattcag agagaccttg atgggacctg ctctctccac accacggcct
     1801 ctaccctaga cgacgatggg aactacacca tcatggctgc caaccctcag ggtcgcgtca
     1861 gttgtacagg aaggctaatg gtacaggctg tcaaccaaag aggccgcagt ccccgctctc
     1921 cctcaggcca tcctcatgcc agaaggcctc gctctcgatc acgggacagt ggagatgaaa
     1981 acgagcccat tcaggagcga ttcttcagac ctcacttcct gcaggctcct ggagacctga
     2041 ccgttcagga aggcaagctc tgcaggatgg actgcaaggt cagtgggtta ccaaccccag
     2101 atctcagctg gcaactagac ggaaagccca tacgccccga cagtgctcac aagatgctgg
     2161 tccgtgagaa tggggtccac tccctcatta tagagccagt cacgtcccgg gacgccggca
     2221 tctacacatg tattgccacc aacagagcag gacagaactc gtttaacctg gagcttgtgg
     2281 ttgctgctaa ggaagcacac aaggcccctg tgtttatgga gaagctacag aacacggggg
     2341 ttgctgatgg atacccagtg cggctagaat gccgtgtctc cggagtgccg ccacctcaga
     2401 tattttggaa gaaagaaaat gaatcgctca ctcatagcac tgagcgagta agcatgcacc
     2461 aggataatca tggctacatc tgcctgctca tccagggagc cacaaaggaa gacgctgggt
     2521 ggtacactgt gtctgccaag aacgaagcag gcattgtgtc ctgcactgcc cggctggatg
     2581 tctacaccca gtggcaccag cagccacaga ccaccaagcc aaaaaaagta cggccctcgg
     2641 ccagtcgcta cgcagcactt tcggaccagg gactagacat caaagccgct ttccaacctg
     2701 aagccagccc atctcacctg actctgaaca gcggcttggt agaaagtgaa gacctgtgag
     2761 aattctgcag tcgacggtac cgcgggcccg ggatccaccg gatctagata actgatcata
     2821 atcagccata ccacattt
//
