LOCUS       sequence-85841-e        2523 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 433, Emission: 475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Markwardt ML, Kremers GJ, Kraft CA, Ray K, Cranfill PJ, Wilson KA, 
            Day RN, Wachter RM, Davidson MW, Rizzo MA
  TITLE     An improved cerulean fluorescent protein with enhanced brightness 
            and reduced reversible photoswitching.
  JOURNAL   PLoS One. 2011  . 6(3):e17896.
  PUBMED    21479270
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2011  . 6(3):e17896."
COMMENT     Sequence Label: mCerulean3-Paxillin-22
FEATURES             Location/Qualifiers
     source          1..2523
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    1698..1750
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      1758..1767
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1764..1766,1767..1769,1770..2483)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIK
                     ANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1809..1830)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2070..2089)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2417..2438
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 acgtcagatc cgctagcacc atggacgacc tcgatgcctt actggcagac ctggaatcca
       61 ccacctcgca tatctctaag cgaccggtgt ttctaacaga ggaaacgcct tactcctacc
      121 caactggaaa ccatacgtac caggagattg ctgtgccacc gccggtgccc ccgccgcctt
      181 ccagtgaggc cctaaatggc actgtgattg accccttaga ccagtggcaa cccagcgtgt
      241 ccagatacgg ccaccagcaa cctcagtccc agtctccgat atacagctct agtgccaaaa
      301 gctccagtgc ctctgttcct agagacgggc tcagctcccc ttctccccgt gccagtgagg
      361 aggaacacgt gtacagcttc ccaaacaagc agaagtctgc agaaccatct cccacaatga
      421 ccagcacctc cctgggcagc aacctctcgg aactggacag gcttctcctg gaactgaatg
      481 ctgttcaaca taatcccccc agtggcttct cagcagatga agtcagcaga agcccatcac
      541 tgcccaatgt gaccggacct cactatgtca tcccagagag cagcagctct gcaggaggga
      601 aggctgcacc ccctacaaaa gaaaaaccaa agcggaatgg tgggcgtggg attgaagatg
      661 tgcgtcccag tgtggagagc ctgctggatg agctggagag ctctgtgcca agcccagtcc
      721 ctgcaatcac tgtgagccaa ggggaggtga gcagccccca gcgtgtcaac gccagtcagc
      781 agcagacccg tatctctgct tcttcagcta cacgagaact ggatgagctg atggcgtccc
      841 tctctgactt taagttcatg gcacagggga aagccggcgg cagcagctcc ccaccatcca
      901 ccaccccaaa gcctggcagt cagctggaca ccatgctggg aagtctgcag tctgacctga
      961 acaaactggg ggtagcgacg gtcgccaagg gtgtgtgcgg agcctgcaag aagccgattg
     1021 ctgggcaggt agttacagcc atggggaaga cctggcaccc tgagcacttc gtctgcaccc
     1081 actgccagga ggagattgga tcacggaact tctttgagcg ggatgggcag ccctactgtg
     1141 agaaggacta tcacaacctc ttctctcctc gctgctacta ctgcaatggg ccaatccttg
     1201 ataaagtggt gacggctttg gacaggacgt ggcaccctga acactttttc tgtgcccagt
     1261 gtggagtttt ctttggacct gaaggatttc atgagaagga tggcaaagcc tattgccgca
     1321 aggactactt tgacatgttt gctcccaagt gtggaggctg tgcccgggct atcctggaaa
     1381 attacatttc tgccttaaac accctgtggc accctgagtg ctttgtctgt cgggaatgtt
     1441 tcacaccgtt catcaacggc agcttcttcg agcacgacgg gcagccctac tgcgaggtgc
     1501 attaccacga gcgccgcggc tcgctgtgct ctggctgcca gaagcccatc acgggacgct
     1561 gcatcactgc catgggcaag aaatttcacc ccgaacactt tgtctgtgcc ttctgcctca
     1621 agcagctcaa caaaggaacc ttcaaagaac agaacgacaa gccctactgt cagaactgct
     1681 ttctcaaact cttctgtctc gagctcaagc ttcgaattct gcagtcgacg gtaccgcggg
     1741 cccgggatcc accggtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg
     1801 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     1861 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
     1921 agctgcccgt gccctggccc accctcgtga ccaccctgag ctggggcgtg cagtgcttcg
     1981 cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     2041 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     2101 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     2161 aggacggcaa catcctgggg cacaagctgg agtacaacgc catccacggc aacgtctata
     2221 tcaccgccga caagcagaag aacggcatca aggccaactt cggcctcaac tgcaacatcg
     2281 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     2341 ccgtgctgct gcccgacaac cactacctga gcacccagtc caagctgagc aaagacccca
     2401 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     2461 gcatggacga gctgtacaag taaagcggcc gcgactctag atcataatca gccataccac
     2521 att
//
