LOCUS       sequence-85846-e        3975 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: FAK/Focal Adhesions, Excitation: 433, Emission: 475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Markwardt ML, Kremers GJ, Kraft CA, Ray K, Cranfill PJ, Wilson KA, 
            Day RN, Wachter RM, Davidson MW, Rizzo MA
  TITLE     An improved cerulean fluorescent protein with enhanced brightness 
            and reduced reversible photoswitching.
  JOURNAL   PLoS One. 2011  . 6(3):e17896.
  PUBMED    21479270
REFERENCE   2  (bases 1 to 3975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2011  . 6(3):e17896."
COMMENT     Sequence Label: mCerulean3-PTK2-C-10
FEATURES             Location/Qualifiers
     source          1..3975
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      21..30
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(27..29,30..32,33..743)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIK
                     ANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(72..93)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(333..352)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     680..701
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atccgctagc gctaccggtc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg
       61 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
      121 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
      181 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gagctggggc gtgcagtgct
      241 tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
      301 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
      361 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
      421 aggaggacgg caacatcctg gggcacaagc tggagtacaa cgccatccac ggcaacgtct
      481 atatcaccgc cgacaagcag aagaacggca tcaaggccaa cttcggcctc aactgcaaca
      541 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
      601 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccaagctg agcaaagacc
      661 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
      721 tcggcatgga cgagctgtac aagtccggac tcagatctag cggtggcagt ggagcagctg
      781 cttaccttga ccccaacttg aatcacacac caaattcgag tactaagact cacctgggta
      841 ctggtatgga acgttctcct ggtgcaatgg agcgagtatt aaaggtcttt cattattttg
      901 aaagcaatag tgagccaacc acctgggcca gtattatcag gcatggagat gctactgatg
      961 tcaggggcat cattcagaag atagtggaca gtcacaaagt aaagcatgtg gcctgctatg
     1021 gattccgcct cagtcacctg cggtcagagg aggttcactg gcttcacgtg gatatgggcg
     1081 tctccagtgt gagggagaag tatgagcttg ctcacccacc agaggagtgg aaatatgaat
     1141 tgagaattcg ttatttgcca aaaggatttc taaaccagtt tactgaagat aagccaactt
     1201 tgaatttctt ctatcaacag gtgaagagcg attatatgtt agagatagct gatcaagtgg
     1261 accaggaaat tgctttgaag ttgggttgtc tagaaatacg gcgatcatac tgggagatgc
     1321 ggggcaatgc actagaaaag aagtctaact atgaagtatt agaaaaagat gttggtttaa
     1381 agcgattttt tcctaagagt ttactggatt ctgtcaaggc caaaacacta agaaaactga
     1441 tccaacaaac atttagacaa tttgccaacc ttaatagaga agaaagtatt ctgaaattct
     1501 ttgagatcct gtctccagtc tacagatttg ataaggaatg cttcaagtgt gctcttggtt
     1561 caagctggat tatttcagtg gaactggcaa tcggcccaga agaaggaatc agttacctaa
     1621 cggacaaggg ctgcaatccc acacatcttg ctgacttcac tcaagtgcaa accattcagt
     1681 attcaaacag tgaagacaag gacagaaaag gaatgctaca actaaaaata gcaggtgcac
     1741 ccgagcctct gacagtgacg gcaccatccc taaccattgc ggagaatatg gctgacctaa
     1801 tagatgggta ctgccggctg gtgaatggaa cctcgcagtc atttatcatc agacctcaga
     1861 aagaaggtga acgggctttg ccatcaatac caaagttggc caacagcgaa aagcaaggca
     1921 tgcggacaca cgccgtctct gtgtcagaaa cagatgatta tgctgagatt atagatgaag
     1981 aagatactta caccatgccc tcaaccaggg attatgagat tcaaagagaa agaatagaac
     2041 ttggacgatg tattggagaa ggccaatttg gagatgtaca tcaaggcatt tatatgagtc
     2101 cagagaatcc agctttggcg gttgcaatta aaacatgtaa aaactgtact tcggacagcg
     2161 tgagagagaa atttcttcaa gaagccttaa caatgcgtca gtttgaccat cctcatattg
     2221 tgaagctgat tggagtcatc acagagaatc ctgtctggat aatcatggag ctgtgcacac
     2281 ttggagagct gaggtcattt ttgcaagtaa ggaaatacag tttggatcta gcatctttga
     2341 tcctgtatgc ctatcagctt agtacagctc ttgcatatct agagagcaaa agatttgtac
     2401 acagggacat tgctgctcgg aatgttctgg tgtcctcaaa tgattgtgta aaattaggag
     2461 actttggatt atcccgatat atggaagata gtacttacta caaagcttcc aaaggaaaat
     2521 tgcctattaa atggatggct ccagagtcaa tcaattttcg acgttttacc tcagctagtg
     2581 acgtatggat gtttggtgtg tgtatgtggg agatactgat gcatggtgtg aagccttttc
     2641 aaggagtgaa gaacaatgat gtaatcggtc gaattgaaaa tggggaaaga ttaccaatgc
     2701 ctccaaattg tcctcctacc ctctacagcc ttatgacgaa atgctgggcc tatgacccca
     2761 gcaggcggcc caggtttact gaacttaaag ctcagctcag cacaatcctg gaggaagaga
     2821 aggctcagca agaagagcgc atgaggatgg agtccagaag acaggccaca gtgtcctggg
     2881 actccggagg gtctgatgaa gcaccgccca agcccagcag accgggttat cccagtccga
     2941 ggtccagcga aggattttat cccagcccac agcacatggt acaaaccaat cattaccagg
     3001 tttctggcta ccctggttca catggaatca cagccatggc tggcagcatc tatccaggtc
     3061 aggcatctct tttggaccaa acagattcat ggaatcatag acctcaggag atagcaatgt
     3121 ggcagcccaa tgtggaggac tctacagtat tggacctgcg agggattggg caagtgttgc
     3181 caacccatct gatggaagag cgtctaatcc gacagcaaca ggaaatggaa gaagatcagc
     3241 gctggctgga aaaagaggaa agatttctga aacctgatgt gagactctct cgaggcagta
     3301 ttgacaggga ggatggaagt cttcagggtc cgattggaaa ccaacatata tatcagcctg
     3361 tgggtaaacc agatcctgca gctccaccaa agaaaccgcc tcgccctgga gctcccggtc
     3421 atctgggaag ccttgccagc ctcagcagcc ctgctgacag ctacaacgag ggtgtcaagc
     3481 catggaggct tcagccccag gaaatcagcc cccctcctac tgccaacctg gaccggtcga
     3541 atgataaggt gtacgagaat gtgacgggcc tggtgaaagc tgtcatcgag atgtccagta
     3601 aaatccagcc agccccacca gaggagtatg tccctatggt gaaggaagtc ggcttggccc
     3661 tgaggacatt attggccact gtggatgaga ccattcccct cctaccagcc agcacccacc
     3721 gagagattga gatggcacag aagctattga actctgacct gggtgagctc atcaacaaga
     3781 tgaaactggc ccagcagtat gtcatgacca gcctccagca agagtacaaa aagcaaatgc
     3841 tgactgctgc tcacgccctg gctgtggatg ccaaaaactt actcgatgtc attgaccaag
     3901 caagactgaa aatgcttggg cagacgagac cacactgagg atccaccgga tctagataac
     3961 tgatcataat cagcc
//
