LOCUS       sequence-85847-e        3976 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: FAK/Focal Adhesions, Excitation: 433, Emission: 475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3976)
  AUTHORS   Markwardt ML, Kremers GJ, Kraft CA, Ray K, Cranfill PJ, Wilson KA, 
            Day RN, Wachter RM, Davidson MW, Rizzo MA
  TITLE     An improved cerulean fluorescent protein with enhanced brightness 
            and reduced reversible photoswitching.
  JOURNAL   PLoS One. 2011  . 6(3):e17896.
  PUBMED    21479270
REFERENCE   2  (bases 1 to 3976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2011  . 6(3):e17896."
COMMENT     Sequence Label: mCerulean3-PTK2-N-10
FEATURES             Location/Qualifiers
     source          1..3976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      16..25
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      3211..3220
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3217..3219,3220..3222,3223..3936)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIK
                     ANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3262..3283)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3523..3542)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3870..3891
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gacagatccg ctagcgccac catggcagct gcttaccttg acccccactt gaatcacaca
       61 ccaaattcga gtactaagac tcacctgggt actggtatgg aacgttctcc tggtgcaatg
      121 gagcgagtat taaaggtctt tcattatttt gaaagcaata gtgagccaac cacctgggcc
      181 agtattatca ggcatggaga tgctactgat gtcaggggca tcattcagaa gatagtggac
      241 agtcacaaag taaagcatgt ggcctgctat ggattccgcc tcagtcacct gcggtcagag
      301 gaggttcact ggcttcacgt ggatatgggc gtctccagtg tgagggagaa gtatgagctt
      361 gctcacccac cagaggagtg gaaatatgaa ttgagaattc gttatttgcc aaaaggattt
      421 ctaaaccagt ttactgaaga taagccaact ttgaatttct tctatcaaca ggtgaagagc
      481 gattatatgt tagagatagc tgatcaagtg gaccaggaaa ttgctttgaa gttgggttgt
      541 ctagaaatac ggcgatcata ctgggagatg cggggcaatg cactagaaaa gaagtctaac
      601 tatgaagtat tagaaaaaga tgttggttta aagcgatttt ttcctaagag tttactggat
      661 tctgtcaagg ccaaaacact aagaaaactg atccaacaaa catttagaca atttgccaac
      721 cttaatagag aagaaagtat tctgaaattc tttgagatcc tgtctccagt ctacagattt
      781 gataaggaat gcttcaagtg tgctcttggt tcaagctgga ttatttcagt ggaactggca
      841 atcggcccag aagaaggaat cagttaccta acggacaagg gctgcaatcc cacacatctt
      901 gctgacttca ctcaagtgca aaccattcag tattcaaaca gtgaagacaa ggacagaaaa
      961 ggaatgctac aactaaaaat agcaggtgca cccgagcctc tgacagtgac ggcaccatcc
     1021 ctaaccattg cggagaatat ggctgaccta atagatgggt actgccggct ggtgaatgga
     1081 acctcgcagt catttatcat cagacctcag aaagaaggtg aacgggcttt gccatcaata
     1141 ccaaagttgg ccaacagcga aaagcaaggc atgcggacac acgccgtctc tgtgtcagaa
     1201 acagatgatt atgctgagat tatagatgaa gaagatactt acaccatgcc ctcaaccagg
     1261 gattatgaga ttcaaagaga aagaatagaa cttggacgat gtattggaga aggccaattt
     1321 ggagatgtac atcaaggcat ttatatgagt ccagagaatc cagctttggc ggttgcaatt
     1381 aaaacatgta aaaactgtac ttcggacagc gtgagagaga aatttcttca agaagcctta
     1441 acaatgcgtc agtttgacca tcctcatatt gtgaagctga ttggagtcat cacagagaat
     1501 cctgtctgga taatcatgga gctgtgcaca cttggagagc tgaggtcatt tttgcaagta
     1561 aggaaataca gtttggatct agcatctttg atcctgtatg cctatcagct tagtacagct
     1621 cttgcatatc tagagagcaa aagatttgta cacagggaca ttgctgctcg gaatgttctg
     1681 gtgtcctcaa atgattgtgt aaaattagga gactttggat tatcccgata tatggaagat
     1741 agtacttact acaaagcttc caaaggaaaa ttgcctatta aatggatggc tccagagtca
     1801 atcaattttc gacgttttac ctcagctagt gacgtatgga tgtttggtgt gtgtatgtgg
     1861 gagatactga tgcatggtgt gaagcctttt caaggagtga agaacaatga tgtaatcggt
     1921 cgaattgaaa atggggaaag attaccaatg cctccaaatt gtcctcctac cctctacagc
     1981 cttatgacga aatgctgggc ctatgacccc agcaggcggc ccaggtttac tgaacttaaa
     2041 gctcagctca gcacaatcct ggaggaagag aaggctcagc aagaagagcg catgaggatg
     2101 gagtccagaa gacaggccac agtgtcctgg gactccggag ggtctgatga agcaccgccc
     2161 aagcccagca gaccgggtta tcccagtccg aggtccagcg aaggatttta tcccagccca
     2221 cagcacatgg tacaaaccaa tcattaccag gtttctggct accctggttc acatggaatc
     2281 acagccatgg ctggcagcat ctatccaggt caggcatctc ttttggacca aacagattca
     2341 tggaatcata gacctcagga gatagcaatg tggcagccca atgtggagga ctctacagta
     2401 ttggacctgc gagggattgg gcaagtgttg ccaacccatc tgatggaaga gcgtctaatc
     2461 cgacagcaac aggaaatgga agaagatcag cgctggctgg aaaaagagga aagatttctg
     2521 aaacctgatg tgagactctc tcgaggcagt attgacaggg aggatggaag tcttcagggt
     2581 ccgattggaa accaacatat atatcagcct gtgggtaaac cagatcctgc agctccacca
     2641 aagaaaccgc ctcgccctgg agctcccggt catctgggaa gccttgccag cctcagcagc
     2701 cctgctgaca gctacaacga gggtgtcaag ccatggaggc ttcagcccca ggaaatcagc
     2761 ccccctccta ctgccaacct ggaccggtcg aatgataagg tgtacgagaa tgtgacgggc
     2821 ctggtgaaag ctgtcatcga gatgtccagt aaaatccagc cagccccacc agaggagtat
     2881 gtccctatgg tgaaggaagt cggcttggcc ctgaggacat tattggccac tgtggatgag
     2941 accattcccc tcctaccagc cagcacccac cgagagattg agatggcaca gaagctattg
     3001 aactctgacc tgggtgagct catcaacaag atgaaactgg cccagcagta tgtcatgacc
     3061 agcctccagc aagagtacaa aaagcaaatg ctgactgctg ctcacgccct ggctgtggat
     3121 gccaaaaact tactcgatgt cattgaccaa gcaagactga aaatgcttgg gcagacgaga
     3181 ccacacagct ccggagggga tccaccggtc gccaccatgg tgagcaaggg cgaggagctg
     3241 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     3301 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     3361 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gagctggggc
     3421 gtgcagtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     3481 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     3541 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     3601 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa cgccatccac
     3661 ggcaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggccaa cttcggcctc
     3721 aactgcaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     3781 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccaagctg
     3841 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     3901 gggatcactc tcggcatgga cgagctgtac aagtaaagcg gccgcgactc tagatcataa
     3961 tcagccatac cacatt
//
