LOCUS       sequence-85848-e        1442 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Ras GTPase, Excitation: 433, Emission: 475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Markwardt ML, Kremers GJ, Kraft CA, Ray K, Cranfill PJ, Wilson KA, 
            Day RN, Wachter RM, Davidson MW, Rizzo MA
  TITLE     An improved cerulean fluorescent protein with enhanced brightness 
            and reduced reversible photoswitching.
  JOURNAL   PLoS One. 2011  . 6(3):e17896.
  PUBMED    21479270
REFERENCE   2  (bases 1 to 1442)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2011  . 6(3):e17896."
COMMENT     Sequence Label: mCerulean3-Rab4a-7
FEATURES             Location/Qualifiers
     source          1..1442
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      14..23
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(20..22,23..25,26..736)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIK
                     ANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(65..86)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(326..345)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     673..694
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 agcgctaccg gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
       61 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
      121 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
      181 gcccgtgccc tggcccaccc tcgtgaccac cctgagctgg ggcgtgcagt gcttcgcccg
      241 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
      301 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
      361 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
      421 cggcaacatc ctggggcaca agctggagta caacgccatc cacggcaacg tctatatcac
      481 cgccgacaag cagaagaacg gcatcaaggc caacttcggc ctcaactgca acatcgagga
      541 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
      601 gctgctgccc gacaaccact acctgagcac ccagtccaag ctgagcaaag accccaacga
      661 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
      721 ggacgagctg tacaagtccg gactcagatc tcgagccatg tcgcagacgg ccatgtccga
      781 aacctacgat tttttgttta agttcttggt tattggaaat gcaggaactg gcaaatcttg
      841 cttacttcat cagtttattg aaaaaaaatt caaagatgac tcaaatcata caataggagt
      901 ggaatttggt tcaaagataa taaatgttgg tggtaaatat gtaaagttac aaatatggga
      961 tacagcagga caagaacgat tcaggtccgt gacgagaagt tattaccgag gcgcggccgg
     1021 ggctctcctc gtctatgata tcaccagccg agaaacctac aatgcgctta ctaattggtt
     1081 aacagatgcc cgaatgctag cgagccagaa cattgtgatc atcctttgtg gaaacaagaa
     1141 ggacctggat gcagatcgtg aagttacctt cttagaagcc tccagatttg ctcaagaaaa
     1201 tgagctgatg tttttggaaa caagtgcgct cacaggggag aatgtagaag aggcttttgt
     1261 acagtgtgca agaaaaatac ttaacaaaat cgaatcaggt gagctggacc cagaaagaat
     1321 gggctcaggt attcagtacg gagatgctgc cttgagacag ctgaggtcac cgcggcgcgc
     1381 acaggccccg aacgctcagg agtgtggttg ttagggatcc accggatcta gataactgat
     1441 ca
//
