LOCUS       sequence-85851-e        2130 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cilium, Excitation: 433, Emission: 475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Markwardt ML, Kremers GJ, Kraft CA, Ray K, Cranfill PJ, Wilson KA, 
            Day RN, Wachter RM, Davidson MW, Rizzo MA
  TITLE     An improved cerulean fluorescent protein with enhanced brightness 
            and reduced reversible photoswitching.
  JOURNAL   PLoS One. 2011  . 6(3):e17896.
  PUBMED    21479270
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2011  . 6(3):e17896."
COMMENT     Sequence Label: mCerulean3-SSTR3-N-17
FEATURES             Location/Qualifiers
     source          1..2130
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1369..1378
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1375..1377,1378..1380,1381..2094)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIK
                     ANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1420..1441)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1681..1700)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2028..2049
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gatccgctag cgctaccgga ctcagatctc gagaccgcca tggccactgt tacctatcct
       61 tcatctgagc ctacgacctt ggaccctggg aacgcatcct cgacctggcc cctggatacc
      121 accctgggga acacatccgc tggcgctagc ctgacaggcc tggctgtcag tggcatcttg
      181 atctctctgg tgtacctggt ggtgtgcgtg gtgggtttgc tgggcaactc gctggtgatc
      241 tacgtggtcc tgcggcacac gtccagccca tcagtgacca gtgtctatat cctcaacctg
      301 gctctggctg atgagctctt catgctaggg ctacccttcc tggctgctca gaacgccctg
      361 tcctactggc ccttcggatc tctcatgtgc cgtctggtca tggccgtgga tggcatcaac
      421 cagttcacca gcatcttctg cctcaccgtc atgagtgtgg accgctatct ggctgtggtg
      481 caccccacac gctcagcccg ctggcgcacg gcaccagtgg ctcgcacggt cagtgcagct
      541 gtctgggtgg cctcggctgt ggtggtgctg cctgtggttg tgttctcagg agtgccccgg
      601 ggcatgagca cgtgccacat gcagtggcca gagccagcgg ctgcctggcg aacagccttc
      661 atcatctaca cggccgcact gggcttcttt gggcccctgc tggtcatctg cttgtgctac
      721 ttgctcattg tggtaaaggt gcggtcgacc acccggcggg tgcgggcgcc ctcgtgtcag
      781 tgggtacagg cacccgcatg ccagcggcga cgccgctctg agcgcagggt cacacgcatg
      841 gtggtggccg tggtggcact cttcgtcctc tgctggatgc ctttctatct gctcaacatc
      901 gtcaatgtgg tgtgcccgct gccggaggag cccgccttct tcggcctcta cttcctggtg
      961 gtggcgctgc cctacgccaa cagctgcgca aaccccatcc tctacggctt cctctcctac
     1021 cgcttcaagc agggctttcg caggatcctg ctaagaccat cacgtcgcat tcggagccag
     1081 gagccagggt cgggacctcc agagaagact gaagaggagg aggatgaaga agaagaagag
     1141 agaagggaag aggaggagcg gaggatgcag agagggcagg agatgaacgg gaggctcagt
     1201 cagatcgcac aggctggcac tagtggacaa cagccacggc cctgcacagg gactgctaag
     1261 gagcagcagc ttctgcccca ggaggccaca gctggggaca aggccagcac actgagccat
     1321 ctgggaattc tgcagtcgac ggtaccgcgg gcccgggatc caccggtcgc caccatggtg
     1381 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     1441 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
     1501 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     1561 accaccctga gctggggcgt gcagtgcttc gcccgctacc ccgaccacat gaagcagcac
     1621 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     1681 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     1741 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     1801 gagtacaacg ccatccacgg caacgtctat atcaccgccg acaagcagaa gaacggcatc
     1861 aaggccaact tcggcctcaa ctgcaacatc gaggacggca gcgtgcagct cgccgaccac
     1921 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     1981 agcacccagt ccaagctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     2041 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaaagcggc
     2101 cgcgactcta gatcataatc agccatacca
//
