LOCUS       sequence-85852-e        8936 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 433, Emission: 475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8936)
  AUTHORS   Markwardt ML, Kremers GJ, Kraft CA, Ray K, Cranfill PJ, Wilson KA, 
            Day RN, Wachter RM, Davidson MW, Rizzo MA
  TITLE     An improved cerulean fluorescent protein with enhanced brightness 
            and reduced reversible photoswitching.
  JOURNAL   PLoS One. 2011  . 6(3):e17896.
  PUBMED    21479270
REFERENCE   2  (bases 1 to 8936)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2011  . 6(3):e17896."
COMMENT     Sequence Label: mCerulean3-Talin-18
FEATURES             Location/Qualifiers
     source          1..8936
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      31..40
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(37..39,40..42,43..753)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIK
                     ANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(82..103)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(343..362)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     690..711
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      802..811
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ttgacgtcag atccgctagc gctaccggtc gccaccatgg tgagcaaggg cgaggagctg
       61 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
      121 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
      181 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gagctggggc
      241 gtgcagtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
      301 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
      361 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
      421 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa cgccatccac
      481 ggcaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggccaa cttcggcctc
      541 aactgcaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
      601 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccaagctg
      661 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
      721 gggatcactc tcggcatgga cgagctgtac aagtccggac tcagatctcg agctcaagct
      781 agcttcgaat tcgcagaagc tgccaccatg gttgcgcttt cgctgaagat tagcattggg
      841 aatgtggtga agacgatgca atttgagcca tctaccatgg tgtacgacgc ctgccgcatg
      901 attcgtgagc ggatcccaga ggccctggct ggccctccca acgactttgg gctctttctg
      961 tcagatgatg accccaaaaa aggcatctgg ctagaggctg ggaaagcttt ggactactac
     1021 atgctccgaa atggggacac catggagtac agaaaaaagc agagacccct gaagatacgg
     1081 atgttagacg gaacggtgaa gactatcatg gtggatgact ccaagaccgt cacagacatg
     1141 ctcatgacca tttgtgcccg aattggtatc accaaccatg atgagtattc actggttcga
     1201 gagctgatgg aagaaaagaa agatgagggg acagggaccc tgagaaaaga caagacccta
     1261 ctgcgagatg aaaagaagat ggagaagcta aagcagaagt tgcacacaga cgatgagttg
     1321 aactggctgg accatgggcg gacactgagg gaacagggag tggaagagca tgagacgttg
     1381 ctgctgcgga gaaagttctt ctactcagac cagaatgtgg attcccgaga ccctgtacag
     1441 ctgaaccttc tctatgtgca ggcacgagat gacatcctga atggctccca tcctgtctcc
     1501 tttgacaagg cctgtgaatt tgcaggcttc cagtgccaga tccagtttgg acctcacaat
     1561 gaacagaagc acaaggctgg cttccttgac ctgaaggact tcctgcccaa ggagtatgtg
     1621 aagcagaagg gagagcgtaa gatctttcag gcacacaaga attgtgggca gatgagtgaa
     1681 attgaggcca aggtacgcta cgtgaagctg gcccgttccc tcaagactta cggtgtctcc
     1741 ttcttcctag tcaaggaaaa gatgaagggg aagaataaac tggtgcccag gctgttgggc
     1801 atcactaagg agtgtgtgat gcgtgtggat gagaagacca aggaggtgat tcaggaatgg
     1861 agcctcacca acatcaaacg ctgggctgcc tctcccaaga gctttactct ggactttgga
     1921 gactatcagg atggctacta ctcagtacag acgacagaag gcgagcagat cgcacagctc
     1981 attgctggct acatagatat catccttaag aagaaaaaaa gcaaggacca ttttgggctg
     2041 gagggagatg aagagtctac tatgttggag gactcagtgt ctcccaaaaa gtcaacagtc
     2101 cttcagcagc agtacaaccg agtggggaaa gtggagcacg gctctgtggc tctgccagcc
     2161 atcatgcgct ctggagcctc tggtcctgag aatttccagg tggggagcat gccacctgcc
     2221 cagcagcaga tcaccagtgg ccagatgcac cgaggacaca tgccacctct gacttcagcc
     2281 cagcaggcgc tcactggaac cattaactcc agcatgcagg ctgtgcaggc tgcccaggcc
     2341 actctggatg actttgagac tctaccccct cttggccagg atgctgcctc caaagcttgg
     2401 cgtaagaaca agatggatga atcaaagcat gagatccact cccaggtaga tgccatcaca
     2461 gctggcactg cctctgtggt aaacctgaca gcaggagatc ctgcagagac agactataca
     2521 gcagtgggct gtgcagtcac caccatctct tccaacctga cggagatgtc ccgtggggtg
     2581 aagctattgg ctgccctgct ggaggatgaa ggcggcaatg gccggcccct tctgcaagca
     2641 gcaaagggcc ttgcaggggc tgtgtcggaa ctgcttcgca gtgctcagcc cgccagcgct
     2701 gagcctcgtc agaacctgct gcaggcagcc gggaacgtgg gccaggccag tggggagctg
     2761 ttgcagcaaa ttggggaaag tgacactgac ccccacttcc aggatgttct aatgcagcta
     2821 gcaaacgcag tggcaagtgc tgcagccgcc ctggtcctca aggccaagag tgtggcccag
     2881 cgcacagagg attccgggct tcagacccaa gtgattgctg cagctacgca atgtgctctg
     2941 tccacttccc agctggtggc ctgtaccaag gtggtagcac ctacaatcag ctcacctgtg
     3001 tgccaagaac agctagtcga ggctgggcga ctggtggcca aagctgtgga gggctgtgtg
     3061 tctgcctcgc aagcagctac agaagatgga cagctgcttc gaggggtagg agcagcagcc
     3121 acggctgtca cccaggccct caatgagctg ctgcagcacg tgaaggccca cgccaccgga
     3181 gctgggcctg ccggtcggta tgaccaagcc actgatacca tcctcaccgt cactgaaaac
     3241 atcttcagct ccatgggtga tgctggggag atggtgcgcc aggcccgcat cctggcccaa
     3301 gccacctcag acctggtcaa tgctatcaag gccgatgctg agggggagag tgatctggag
     3361 aactctagaa agctcctgag tgctgccaag atcctcgctg atgccaccgc caagatggtg
     3421 gaggcggcca agggagcagc cgcccaccct gacagtgagg aacagcagca gcgactgcgt
     3481 gaagcagctg aggggcttcg catggccacc aatgcagctg cgcagaacgc catcaagaag
     3541 aagttggtgc agcgcctgga gcatgcagcc aagcaagctg cagcctctgc aacacagacc
     3601 attgctgcag cccaacatgc agcctctgcc cccaaggcct ctgcgggccc ccagccccta
     3661 ctggtgcaga gctgtaaggc cgtggcagag cagattccgt tgctggtgca gggtgtccga
     3721 ggaagtcaag ctcagcctga cagccctagt gctcagctcg ccctcattgc tgccagccag
     3781 agcttcctgc agccaggtgg gaagatggtg gcagcggcaa aagcctcagt gccaacaatt
     3841 caggaccagg cttcagcgat gcagctgagt cagtgcgcaa agaacctagg cactgccctg
     3901 gccgagctgc gcactgctgc tcagaaggct caggaggcat gtggaccttt ggagatggat
     3961 tctgcactga gtgttgtaca aaatctagag aaagacctac aggaaataaa agcagcagct
     4021 cgagatggca agcttaagcc cttacctggg gaaacaatgg agaagtgtac ccaagatctt
     4081 ggcaacagca ccaaagcggt gagctctgcc atcgccaagc tgctggggga gattgcccag
     4141 ggcaacgaga actatgcagg tattgcagct cgggatgtag ctggtggact aaggtcacta
     4201 gcccaggctg cgcggggtgt ggctgcgctg acatcagatc ctgcagtgca ggccattgtg
     4261 cttgacacag ccagcgacgt tctggacaag gccagcagcc tcattgaaga ggcaaaaaaa
     4321 gcatctggac acccagggga cccagaaagc cagcaaaggc ttgctcaggt agctaaagca
     4381 gtgacccaag ccctgaaccg ctgtgtcagc tgcctgcctg gccaaagaga cgtggataat
     4441 gccctacgag ccgttggaga tgccagcaag cgactcctga gtgacttgct tcctccaagc
     4501 acggggacat ttcaagaagc acagagccga ttgaatgaag ctgcagctgg gttaaatcag
     4561 gccgccacag aactggtgca agcctcccga ggaactcctc aggacctggc tcgggcctcc
     4621 ggccgatttg gacaggactt cagcaccttc ctggaagctg gtgtggagat ggccggacag
     4681 gccccgagcc aggaggaccg agcccaggtg gtgtccaacc tgaagggcat atccatgtct
     4741 tcaagcaaac ttctccttgc cgctaaggcc ttgtccacag accctgcttc tcccaacctc
     4801 aagagtcagc tggctgcggc tgcccgggca gtgacggaca gcatcaacca gctcatcacc
     4861 atgtgcaccc agcaggcacc tggccagaag gagtgtgaca atgcacttcg gcagctagag
     4921 acagtccgag aactcctgga gaatccagtc cagcccatca acgacatgtc ctacttcggt
     4981 tgcttggaca gtgtcatgga gaactccaag gtcctaggtg aggccatgac tggcatctcc
     5041 caaaatgcca agaatggaaa tctgccggag tttggagatg ccattgccac agcctccaag
     5101 gctctctgtg gcttcacgga ggcagccgca caggcagcat atctagttgg tgtctctgac
     5161 cccaacagcc aagctggaca gcaaggactg gtggaaccca cacagtttgc ccgtgcaaac
     5221 caggcaattc agatggcctg tcagagtctg ggggagcctg gctgtaccca ggcccaggtg
     5281 ttatctgcag ccactattgt agccaaacac acatctgcat tgtgtaacag ctgtcgcctg
     5341 gcttccgcta ggactgccaa tcccactgcc aagcgccagt ttgtacagtc agccaaggag
     5401 gtggccaaca gtacagccaa tcttgtcaag accatcaagg cactagatgg ggacttcaca
     5461 gaagagaacc gtgcccagtg ccgagcagcc acagcccctc tgctggaagc tgtggataac
     5521 ctgagtgcct ttgcctccaa ccctgagttc tccagcgtcc ctgcccagat cagccctgag
     5581 ggcagagcag ccatggagcc cattgtaatc tctgctaaga caatgttgga gagtgctgga
     5641 gggctcatcc agacagctcg ggcgttagca gtcaatcccc gagacccccc acgttggtct
     5701 gtgctagctg gccactctcg gactgtctca gattccatca agaaacttat tacaagcatg
     5761 agggacaaag ccccagggca gctggagtgt gagacagcca ttgcggctct gaacagctgc
     5821 ttgcgggacc tagatcaggc ttcgcttgcc gctgtcagcc agcagcttgc tccccgtgaa
     5881 ggaatctctc aagaggcttt gcacacccag atgctcactg cagtgcaaga aatttctcat
     5941 ctcattgagc cgctggccag cgctgctcgg gctgaagcct cccagctggg acacaaggtg
     6001 tcccaaatgg cccagtactt tgaaccactc accctggctg cagtgggtgc tgcgtctaag
     6061 accctgagcc acccgcagca gatggcactt ctggaccaga ctaaaacgtt ggcagagtct
     6121 gccttgcagt tgctatatac tgccaaggag gctggtggta accccaagca agcagcacac
     6181 acccaggaag ccctggagga ggctgtgcag atgatgacag aggccgtaga agacctgacg
     6241 acgaccctca acgaagcagc cagtgctgca ggagtcgttg gcggcatggt ggactctatc
     6301 actcaggcca tcaaccagct agatgaagga cctatgggtg acccagaagg ctcattcgta
     6361 gattaccaga caaccatggt gaggacagcc aaggccattg ctgtcactgt tcaggagatg
     6421 gtaaccaagt caaacaccag ccctgaagag ctaggccctc ttgccaacca gctgaccagt
     6481 gactatggcc gactggcctc acaagccaag cctgcagctg tggctgctga aaatgaagag
     6541 ataggcgctc acatcaaaca ccgagtacag gagctgggcc atggctgctc tgctctggtc
     6601 accaaggcag gtgccttgca gtgtagcccc agtgatgtct acaccaagaa ggagctcata
     6661 gagtgtgccc gcagagtgtc agagaaggtc tcccatgttc tggctgcact ccaggctggg
     6721 aatcgtggta cccaggcctg cattacagca gccagtgctg tgtctggtat cattgctgac
     6781 ctcgacacca ccatcatgtt cgctactgct ggcacactta accgtgaggg tgctgaaact
     6841 tttgctgacc accgggaggg tatcttaaag acagcaaagg ttcttgtgga ggacaccaag
     6901 gtcctagtgc agaatgcagc tgggagccag gagaagttgg cacaagccgc ccagtcctcc
     6961 gtggccacca ttacccgcct cgctgatgtg gtcaagctcg gtgcagccag cctaggagcc
     7021 gaagaccctg aaactcaggt ggtgctgatc aatgcagtaa aggacgtagc caaggccctg
     7081 ggtgacctca tcagcgctac gaaggctgca gcgggcaaag ttggggatga ccctgcagtg
     7141 tggcagctca agaactctgc caaggtgatg gtgaccaatg tgacatcatt gctcaagaca
     7201 gtgaaggctg tggaagatga ggccaccaaa ggcacacggg ccctagaggc aaccacagag
     7261 cacatacgtc aggaactggc ggtcttctgt tccccagagc cacctgccaa gacctctacc
     7321 cctgaagact tcatccgaat gaccaagggt attactatgg caacagccaa agccgttgct
     7381 gctggcaatt cctgtcgaca ggaagatgtc attgccacag ccaatctgag ccgacgggct
     7441 attgcggaca tgcttcgggc ttgcaaggaa gcagctttcc acccagaagt ggcgcctgat
     7501 gtacggctcc gagccctgca ctatggccgg gagtgtgcca atggttacct ggaactgctg
     7561 gaccacgtgc tgctgaccct tcagaagcca aacccagacc tgaagcagca gctaactgga
     7621 cactcaaagc gggttgctgg ctctgtgact gagctcatcc aggctgctga agccatgaaa
     7681 ggaacagagt gggtggaccc agaggaccct actgtcattg ctgagaatga gctcttggga
     7741 gccgcagccg ccatcgaggc tgcagccaag aagctggagc agctgaagcc cagggccaaa
     7801 cccaaggagg cggatgagtc cttgaacttt gaggaacaaa tcctagaagc tgccaagtcc
     7861 atcgctgcag ccaccagtgc actggtaaag gctgcgtcag cagcccagag ggagctggtg
     7921 gctcaaggaa aggtgggcgc cattccagcc aatgcactgg atgatgggca gtggtcgcag
     7981 ggcctcattt ccgctgcccg tatggtggct gcggccacca acaatctgtg tgaagcagct
     8041 aacgcagctg tccagggcca tgctagccag gagaaactca tttcctcagc caagcaagta
     8101 gctgcctcca cagctcagct cctggtagct tgcaaggtca aagcagatca ggactctgag
     8161 gcaatgaaac ggctccaggc tgctggcaat gcagtgaaga gggcctcaga taacctggta
     8221 aaggcggccc agaaagctgc agccttcgaa gaccaggaga atgagacggt ggtggtgaag
     8281 gagaaaatgg ttgggggcat tgcccagatt atcgcagcac aggaagagat gcttcggaag
     8341 gaacgagagc tggaagaggc tcggaaaaag ctcgcccaga tccggcagca gcagtacaag
     8401 ttcttgcctt cagagcttcg agacgagcac taaaaaagcc ctgtgtattt aatgcagacc
     8461 cagcccagag actgtgcctg ccactaccaa agccttctgg gctgctcagg acccaagctg
     8521 cccaacccca gcactccccc aaagtgcctg ccaaaccctg ggcctggccc tgcccagtcc
     8581 cactgcaagc cgtgtcctct ccctgactcc caagtgcctt tgcaccctag ggcccctaag
     8641 tgcctgcccc cctccagagt attaacgctc caagagtatt attaatgctg ccgtacctca
     8701 gtttgaacct gccagggccc cagctgctcc agcctgccag cagcttccag ccagtcccca
     8761 cggccacgtc agctcaactc atcccttttt gatactatat cccctaccca gctacctatg
     8821 gggcttgagg gttgtaaacc caaacaggtc agactcccgg aattctgcag tcgacggtac
     8881 cgcgggcccg ggatccaccg gatctagata actgatcata atcagccata ccacat
//
