LOCUS       sequence-85853-e        8469 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 433, Emission: 475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8469)
  AUTHORS   Markwardt ML, Kremers GJ, Kraft CA, Ray K, Cranfill PJ, Wilson KA, 
            Day RN, Wachter RM, Davidson MW, Rizzo MA
  TITLE     An improved cerulean fluorescent protein with enhanced brightness 
            and reduced reversible photoswitching.
  JOURNAL   PLoS One. 2011  . 6(3):e17896.
  PUBMED    21479270
REFERENCE   2  (bases 1 to 8469)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2011  . 6(3):e17896."
COMMENT     Sequence Label: mCerulean3-Talin-N-10
FEATURES             Location/Qualifiers
     source          1..8469
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      52..61
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      7705..7714
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(7711..7713,7714..7716,7717..8430)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIK
                     ANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7756..7777)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8017..8036)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8364..8385
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gatccgctag cgctaccgga ctcagatctc gagctcaagc ttcgaattct agccaccatg
       61 gttgcgcttt cgctgaagat tagcattggg aatgtggtga agacgatgca atttgagcca
      121 tctaccatgg tgtacgacgc ctgccgcatg attcgtgagc ggatcccaga ggccctggct
      181 ggccctccca acgactttgg gctctttctg tcagatgatg accccaaaaa aggcatctgg
      241 ctagaggctg ggaaagcttt ggactactac atgctccgaa atggggacac catggagtac
      301 agaaaaaagc agagacccct gaagatacgg atgttagacg gaacggtgaa gactatcatg
      361 gtggatgact ccaagaccgt cacagacatg ctcatgacca tttgtgcccg aattggtatc
      421 accaaccatg atgagtattc actggttcga gagctgatgg aagaaaagaa agatgagggg
      481 acagggaccc tgagaaaaga caagacccta ctgcgagatg aaaagaagat ggagaagcta
      541 aagcagaagt tgcacacaga cgatgagttg aactggctgg accatgggcg gacactgagg
      601 gaacagggag tggaagagca tgagacgttg ctgctgcgga gaaagttctt ctactcagac
      661 cagaatgtgg attcccgaga ccctgtacag ctgaaccttc tctatgtgca ggcacgagat
      721 gacatcctga atggctccca tcctgtctcc tttgacaagg cctgtgaatt tgcaggcttc
      781 cagtgccaga tccagtttgg acctcacaat gaacagaagc acaaggctgg cttccttgac
      841 ctgaaggact tcctgcccaa ggagtatgtg aagcagaagg gagagcgtaa gatctttcag
      901 gcacacaaga attgtgggca gatgagtgaa attgaggcca aggtacgcta cgtgaagctg
      961 gcccgttccc tcaagactta cggtgtctcc ttcttcctag tcaaggaaaa gatgaagggg
     1021 aagaataaac tggtgcccag gctgttgggc atcactaagg agtgtgtgat gcgtgtggat
     1081 gagaagacca aggaggtgat tcaggaatgg agcctcacca acatcaaacg ctgggctgcc
     1141 tctcccaaga gctttactct ggactttgga gactatcagg atggctacta ctcagtacag
     1201 acgacagaag gcgagcagat cgcacagctc attgctggct acatagatat catccttaag
     1261 aagaaaaaaa gcaaggacca ttttgggctg gagggagatg aagagtctac tatgttggag
     1321 gactcagtgt ctcccaaaaa gtcaacagtc cttcagcagc agtacaaccg agtggggaaa
     1381 gtggagcacg gctctgtggc tctgccagcc atcatgcgct ctggagcctc tggtcctgag
     1441 aatttccagg tggggagcat gccacctgcc cagcagcaga tcaccagtgg ccagatgcac
     1501 cgaggacaca tgccacctct gacttcagcc cagcaggcgc tcactggaac cattaactcc
     1561 agcatgcagg ctgtgcaggc tgcccaggcc actctggatg actttgagac tctaccccct
     1621 cttggccagg atgctgcctc caaagcttgg cgtaagaaca agatggatga atcaaagcat
     1681 gagatccact cccaggtaga tgccatcaca gctggcactg cctctgtggt aaacctgaca
     1741 gcaggagatc ctgcagagac agactataca gcagtgggct gtgcagtcac caccatctct
     1801 tccaacctga cggagatgtc ccgtggggtg aagctattgg ctgccctgct ggaggatgaa
     1861 ggcggcaatg gccggcccct tctgcaagca gcaaagggcc ttgcaggggc tgtgtcggaa
     1921 ctgcttcgca gtgctcagcc cgccagcgct gagcctcgtc agaacctgct gcaggcagcc
     1981 gggaacgtgg gccaggccag tggggagctg ttgcagcaaa ttggggaaag tgacactgac
     2041 ccccacttcc aggatgttct aatgcagcta gcaaacgcag tggcaagtgc tgcagccgcc
     2101 ctggtcctca aggccaagag tgtggcccag cgcacagagg attccgggct tcagacccaa
     2161 gtgattgctg cagctacgca atgtgctctg tccacttccc agctggtggc ctgtaccaag
     2221 gtggtagcac ctacaatcag ctcacctgtg tgccaagaac agctagtcga ggctgggcga
     2281 ctggtggcca aagctgtgga gggctgtgtg tctgcctcgc aagcagctac agaagatgga
     2341 cagctgcttc gaggggtagg agcagcagcc acggctgtca cccaggccct caatgagctg
     2401 ctgcagcacg tgaaggccca cgccaccgga gctgggcctg ccggtcggta tgaccaagcc
     2461 actgatacca tcctcaccgt cactgaaaac atcttcagct ccatgggtga tgctggggag
     2521 atggtgcgcc aggcccgcat cctggcccaa gccacctcag acctggtcaa tgctatcaag
     2581 gccgatgctg agggggagag tgatctggag aactctagaa agctcctgag tgctgccaag
     2641 atcctcgctg atgccaccgc caagatggtg gaggcggcca agggagcagc cgcccaccct
     2701 gacagtgagg aacagcagca gcgactgcgt gaagcagctg aggggcttcg catggccacc
     2761 aatgcagctg cgcagaacgc catcaagaag aagttggtgc agcgcctgga gcatgcagcc
     2821 aagcaagctg cagcctctgc aacacagacc attgctgcag cccaacatgc agcctctgcc
     2881 cccaaggcct ctgcgggccc ccagccccta ctggtgcaga gctgtaaggc cgtggcagag
     2941 cagattccgt tgctggtgca gggtgtccga ggaagtcaag ctcagcctga cagccctagt
     3001 gctcagctcg ccctcattgc tgccagccag agcttcctgc agccaggtgg gaagatggtg
     3061 gcagcggcaa aagcctcagt gccaacaatt caggaccagg cttcagcgat gcagctgagt
     3121 cagtgcgcaa agaacctagg cactgccctg gccgagctgc gcactgctgc tcagaaggct
     3181 caggaggcat gtggaccttt ggagatggat tctgcactga gtgttgtaca aaatctagag
     3241 aaagacctac aggaaataaa agcagcagct cgagatggca agcttaagcc cttacctggg
     3301 gaaacaatgg agaagtgtac ccaagatctt ggcaacagca ccaaagcggt gagctctgcc
     3361 atcgccaagc tgctggggga gattgcccag ggcaacgaga actatgcagg tattgcagct
     3421 cgggatgtag ctggtggact aaggtcacta gcccaggctg cgcggggtgt ggctgcgctg
     3481 acatcagatc ctgcagtgca ggccattgtg cttgacacag ccagcgacgt tctggacaag
     3541 gccagcagcc tcattgaaga ggcaaaaaaa gcatctggac acccagggga cccagaaagc
     3601 cagcaaaggc ttgctcaggt agctaaagca gtgacccaag ccctgaaccg ctgtgtcagc
     3661 tgcctgcctg gccaaagaga cgtggataat gccctacgag ccgttggaga tgccagcaag
     3721 cgactcctga gtgacttgct tcctccaagc acggggacat ttcaagaagc acagagccga
     3781 ttgaatgaag ctgcagctgg gttaaatcag gccgccacag aactggtgca agcctcccga
     3841 ggaactcctc aggacctggc tcgggcctcc ggccgatttg gacaggactt cagcaccttc
     3901 ctggaagctg gtgtggagat ggccggacag gccccgagcc aggaggaccg agcccaggtg
     3961 gtgtccaacc tgaagggcat atccatgtct tcaagcaaac ttctccttgc cgctaaggcc
     4021 ttgtccacag accctgcttc tcccaacctc aagagtcagc tggctgcggc tgcccgggca
     4081 gtgacggaca gcatcaacca gctcatcacc atgtgcaccc agcaggcacc tggccagaag
     4141 gagtgtgaca atgcacttcg gcagctagag acagtccgag aactcctgga gaatccagtc
     4201 cagcccatca acgacatgtc ctacttcggt tgcttggaca gtgtcatgga gaactccaag
     4261 gtcctaggtg aggccatgac tggcatctcc caaaatgcca agaatggaaa tctgccggag
     4321 tttggagatg ccattgccac agcctccaag gctctctgtg gcttcacgga ggcagccgca
     4381 caggcagcat atctagttgg tgtctctgac cccaacagcc aagctggaca gcaaggactg
     4441 gtggaaccca cacagtttgc ccgtgcaaac caggcaattc agatggcctg tcagagtctg
     4501 ggggagcctg gctgtaccca ggcccaggtg ttatctgcag ccactattgt agccaaacac
     4561 acatctgcat tgtgtaacag ctgtcgcctg gcttccgcta ggactgccaa tcccactgcc
     4621 aagcgccagt ttgtacagtc agccaaggag gtggccaaca gtacagccaa tcttgtcaag
     4681 accatcaagg cactagatgg ggacttcaca gaagagaacc gtgcccagtg ccgagcagcc
     4741 acagcccctc tgctggaagc tgtggataac ctgagtgcct ttgcctccaa ccctgagttc
     4801 tccagcgtcc ctgcccagat cagccctgag ggcagagcag ccatggagcc cattgtaatc
     4861 tctgctaaga caatgttgga gagtgctgga gggctcatcc agacagctcg ggcgttagca
     4921 gtcaatcccc gagacccccc acgttggtct gtgctagctg gccactctcg gactgtctca
     4981 gattccatca agaaacttat tacaagcatg agggacaaag ccccagggca gctggagtgt
     5041 gagacagcca ttgcggctct gaacagctgc ttgcgggacc tagatcaggc ttcgcttgcc
     5101 gctgtcagcc agcagcttgc tccccgtgaa ggaatctctc aagaggcttt gcacacccag
     5161 atgctcactg cagtgcaaga aatttctcat ctcattgagc cgctggccag cgctgctcgg
     5221 gctgaagcct cccagctggg acacaaggtg tcccaaatgg cccagtactt tgaaccactc
     5281 accctggctg cagtgggtgc tgcgtctaag accctgagcc acccgcagca gatggcactt
     5341 ctggaccaga ctaaaacgtt ggcagagtct gccttgcagt tgctatatac tgccaaggag
     5401 gctggtggta accccaagca agcagcacac acccaggaag ccctggagga ggctgtgcag
     5461 atgatgacag aggccgtaga agacctgacg acgaccctca acgaagcagc cagtgctgca
     5521 ggagtcgttg gcggcatggt ggactctatc actcaggcca tcaaccagct agatgaagga
     5581 cctatgggtg acccagaagg ctcattcgta gattaccaga caaccatggt gaggacagcc
     5641 aaggccattg ctgtcactgt tcaggagatg gtaaccaagt caaacaccag ccctgaagag
     5701 ctaggccctc ttgccaacca gctgaccagt gactatggcc gactggcctc acaagccaag
     5761 cctgcagctg tggctgctga aaatgaagag ataggcgctc acatcaaaca ccgagtacag
     5821 gagctgggcc atggctgctc tgctctggtc accaaggcag gtgccttgca gtgtagcccc
     5881 agtgatgtct acaccaagaa ggagctcata gagtgtgccc gcagagtgtc agagaaggtc
     5941 tcccatgttc tggctgcact ccaggctggg aatcgtggta cccaggcctg cattacagca
     6001 gccagtgctg tgtctggtat cattgctgac ctcgacacca ccatcatgtt cgctactgct
     6061 ggcacactta accgtgaggg tgctgaaact tttgctgacc accgggaggg tatcttaaag
     6121 acagcaaagg ttcttgtgga ggacaccaag gtcctagtgc agaatgcagc tgggagccag
     6181 gagaagttgg cacaagccgc ccagtcctcc gtggccacca ttacccgcct cgctgatgtg
     6241 gtcaagctcg gtgcagccag cctaggagcc gaagaccctg aaactcaggt ggtgctgatc
     6301 aatgcagtaa aggacgtagc caaggccctg ggtgacctca tcagcgctac gaaggctgca
     6361 gcgggcaaag ttggggatga ccctgcagtg tggcagctca agaactctgc caaggtgatg
     6421 gtgaccaatg tgacatcatt gctcaagaca gtgaaggctg tggaagatga ggccaccaaa
     6481 ggcacacggg ccctagaggc aaccacagag cacatacgtc aggaactggc ggtcttctgt
     6541 tccccagagc cacctgccaa gacctctacc cctgaagact tcatccgaat gaccaagggt
     6601 attactatgg caacagccaa agccgttgct gctggcaatt cctgtcgaca ggaagatgtc
     6661 attgccacag ccaatctgag ccgacgggct attgcggaca tgcttcgggc ttgcaaggaa
     6721 gcagctttcc acccagaagt ggcacctgat gtacggctcc gagccctgca ctatggccgg
     6781 gagtgtgcca atggttacct ggaactgctg gaccacgtgc tgctgaccct tcagaagcca
     6841 aacccagacc tgaagcagca gctaactgga cactcaaagc gggttgctgg ctctgtgact
     6901 gagctcatcc aggctgctga agccatgaaa ggaacagagt gggtggaccc agaggaccct
     6961 actgtcattg ctgagaatga gctcttggga gccgcagccg ccatcgaggc tgcagccaag
     7021 aagctggagc agctgaagcc cagggccaaa cccaaggagg cggatgagtc cttgaacttt
     7081 gaggaacaaa tcctagaagc tgccaagtcc atcgctgcag ccaccagtgc actggtaaag
     7141 gctgcgtcag cagcccagag ggagctggtg gctcaaggaa aggtgggcgc cattccagcc
     7201 aatgcactgg atgatgggca gtggtcgcag ggcctcattt ccgctgcccg tatggtggct
     7261 gcggccacca acaatctgtg tgaagcagct aacgcagctg tccagggcca tgctagccag
     7321 gagaaactca tttcctcagc caagcaagta gctgcctcca cagctcagct cctggtagct
     7381 tgcaaggtca aagcagatca ggactctgag gcaatgaaac ggctccaggc tgctggcaat
     7441 gcagtgaaga gggcctcaga taacctggta aaggcggccc agaaagctgc agccttcgaa
     7501 gaccaggaga atgagacggt ggtggtgaag gagaaaatgg ttgggggcat tgcccagatt
     7561 atcgcagcac aggaagagat gcttcggaag gaacgagagc tggaagaggc tcggaaaaag
     7621 ctcgcccaga tccggcagca gcagtacaag ttcttgcctt cagagcttcg agacgagcgc
     7681 ggctccgcgt cccgatcacc ggtcgccacc atggtgagca agggcgagga gctgttcacc
     7741 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     7801 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     7861 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgagctg gggcgtgcag
     7921 tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     7981 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     8041 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     8101 ttcaaggagg acggcaacat cctggggcac aagctggagt acaacgccat ccacggcaac
     8161 gtctatatca ccgccgacaa gcagaagaac ggcatcaagg ccaacttcgg cctcaactgc
     8221 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     8281 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccaa gctgagcaaa
     8341 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     8401 actctcggca tggacgagct gtacaagtaa agcggccgcg actctagatc ataatcagcc
     8461 ataccacat
//
