LOCUS       sequence-85857-e        2232 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Intermediate Filaments, Excitation: 433, Emission: 
            475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Markwardt ML, Kremers GJ, Kraft CA, Ray K, Cranfill PJ, Wilson KA, 
            Day RN, Wachter RM, Davidson MW, Rizzo MA
  TITLE     An improved cerulean fluorescent protein with enhanced brightness 
            and reduced reversible photoswitching.
  JOURNAL   PLoS One. 2011  . 6(3):e17896.
  PUBMED    21479270
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2011  . 6(3):e17896."
COMMENT     Sequence Label: mCerulean3-Vimentin-7
FEATURES             Location/Qualifiers
     source          1..2232
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1466..1475
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1472..1474,1475..1477,1478..2191)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIK
                     ANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1517..1538)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1778..1797)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2125..2146
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 agatccgcta gcgctaccgg actcagatct cgagctcaag cttcgaattc ccatgtccac
       61 caggtccgtg tcctcgtcct cctaccgcag gatgttcggc ggcccgggca ccgcgagccg
      121 gccgagctcc agccggagct acgtgactac gtccacccgc acctacagcc tgggcagcgc
      181 gctgcgcccc agcaccagcc gcagcctcta cgcctcgtcc ccgggcggcg tgtatgccac
      241 gcgctcctct gccgtgcgcc tgcggagcag cgtgcccggg gtgcggctcc tgcaggactc
      301 ggtggacttc tcgctggccg acgccatcaa caccgagttc aagaacaccc gcaccaacga
      361 gaaggtggag ctgcaggagc tgaatgaccg cttcgccaac tacatcgaca aggtgcgctt
      421 cctggagcag cagaataaga tcctgctggc cgagctcgag cagctcaagg gccaaggcaa
      481 gtcgcgcctg ggggacctct acgaggagga gatgcgggag ctgcgccggc aggtggacca
      541 gctaaccaac gacaaagccc gcgtcgaggt ggagcgcgac aacctggccg aggacatcat
      601 gcgcctccgg gagaaattgc aggaggagat gcttcagaga gaggaagccg aaaacaccct
      661 gcaatctttc agacaggatg ttgacaatgc gtctctggca cgtcttgacc ttgaacgcaa
      721 agtggaatct ttgcaagaag agattgcctt tttgaagaaa ctccacgaag aggaaatcca
      781 ggagctgcag gctcagattc aggaacagca tgtccaaatc gatgtggatg tttccaagcc
      841 tgacctcacg gctgccctgc gtgacgtacg tcagcaatat gaaagtgtgg ctgccaagaa
      901 cctgcaggag gcagaagaat ggtacaaatc caagtttgct gacctctctg aggctgccaa
      961 ccggaacaat gacgccctgc gccaggcaaa gcaggagtcc actgagtacc ggagacaggt
     1021 gcagtccctc acctgtgaag tggatgccct taaaggaacc aatgagtccc tggaacgcca
     1081 gatgcgtgaa atggaagaga actttgccgt tgaagctgct aactaccaag acactattgg
     1141 ccgcctgcag gatgagattc agaatatgaa ggaggaaatg gctcgtcacc ttcgtgaata
     1201 ccaagacctg ctcaatgtta agatggccct tgacattgag attgccacct acaggaagct
     1261 gctggaaggc gaggagagca ggatttctct gcctcttcca aacttttcct ccctgaacct
     1321 gagggaaact aatctggatt cactccctct ggttgatacc cactcaaaaa ggacacttct
     1381 gattaagacg gttgaaacta gagatggaca ggttatcaac gaaacttctc agcatcacga
     1441 tgaccttgaa ggggatccac cggtcgccac catggtgagc aagggcgagg agctgttcac
     1501 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     1561 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     1621 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgagct ggggcgtgca
     1681 gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     1741 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     1801 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     1861 cttcaaggag gacggcaaca tcctggggca caagctggag tacaacgcca tccacggcaa
     1921 cgtctatatc accgccgaca agcagaagaa cggcatcaag gccaacttcg gcctcaactg
     1981 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     2041 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtcca agctgagcaa
     2101 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     2161 cactctcggc atggacgagc tgtacaagta aagcggccgc gactctagat cataatcagc
     2221 cataccacaa tt
//
