LOCUS       sequence-85858-e        4061 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 433, Emission: 475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4061)
  AUTHORS   Markwardt ML, Kremers GJ, Kraft CA, Ray K, Cranfill PJ, Wilson KA, 
            Day RN, Wachter RM, Davidson MW, Rizzo MA
  TITLE     An improved cerulean fluorescent protein with enhanced brightness 
            and reduced reversible photoswitching.
  JOURNAL   PLoS One. 2011  . 6(3):e17896.
  PUBMED    21479270
REFERENCE   2  (bases 1 to 4061)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2011  . 6(3):e17896."
COMMENT     Sequence Label: mCerulean3-Vinculin-23
FEATURES             Location/Qualifiers
     source          1..4061
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      30..39
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(36..38,39..41,42..752)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIK
                     ANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(81..102)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(342..361)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     689..710
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tgacgtcaga tccgctagcg ctaccggtcg ccaccatggt gagcaagggc gaggagctgt
       61 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
      121 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
      181 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg agctggggcg
      241 tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
      301 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
      361 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
      421 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac gccatccacg
      481 gcaacgtcta tatcaccgcc gacaagcaga agaacggcat caaggccaac ttcggcctca
      541 actgcaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
      601 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccaagctga
      661 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
      721 ggatcactct cggcatggac gagctgtaca agtccggact cagatctcga gctcaagctt
      781 cgaattcggg aaactcgaga acaaagcttg aaaacggccg catgccagtg tttcatacgc
      841 gcacgatcga gagcatcctg gagccggtgg cacagcagat ctcccacctg gtgataatgc
      901 acgaggaggg cgaggtggac ggcaaagcca ttcctgacct caccgcgccc gtggccgccg
      961 tgcaggcggc cgtcagcaac ctcgtccggg ttggaaaaga gactgttcaa accactgagg
     1021 atcagatttt gaagagagat atgccaccag catttattaa ggttgagaat gcttgcacca
     1081 agcttgtcca ggcagctcag atgcttcagt cagaccctta ctcagtgcct gctcgagatt
     1141 atctaattga tgggtcaagg ggcatcctct ctggaacatc agacctgctc cttaccttcg
     1201 atgaggctga ggtccgtaaa attattagag tttgcaaagg aattttggaa tatcttacag
     1261 tggcagaggt ggtggagact atggaagatt tggtcactta cacaaagaat cttgggccag
     1321 gaatgactaa gatggccaag atgattgacg agagacagca ggagctcact caccaggagc
     1381 accgagtgat gttggtgaac tcgatgaaca ccgtgaaaga gttgctgcca gttctcattt
     1441 cagctatgaa gatttttgta acaactaaaa actcaaaaaa ccaaggcata gaggaagctt
     1501 taaaaaatcg caattttact gtagaaaaaa tgagtgctga aattaatgag ataattcgtg
     1561 tgttacaact cacctcttgg gatgaagatg cctgggccag caaggacact gaagccatga
     1621 agagagcatt ggcctccata gactccaaac tgaaccaggc caaaggttgg ctccgtgacc
     1681 ctagtgcctc cccaggggat gctggtgagc aggccatcag acagatctta gatgaagctg
     1741 gaaaagttgg tgaactctgt gcaggcaaag aacgcaggga gattctggga acttgcaaaa
     1801 tgctagggca gatgactgat caagtggctg acctccgtgc cagaggacaa ggatcctcac
     1861 cggtggccat gcagaaagct cagcaggtat ctcagggtct ggatgtgctc acagcaaaag
     1921 tggaaaatgc agctcgcaag ctggaagcca tgaccaactc aaagcagagc attgcaaaga
     1981 agatcgatgc tgctcagaac tggcttgcag atccaaatgg tggaccggaa ggagaagagc
     2041 agattcgagg tgctttggct gaagctcgga aaatagcaga attatgtgat gatcctaaag
     2101 aaagagatga cattctacgt tcccttgggg aaatatctgc tctgacttct aaattagcag
     2161 atctacgaag acaggggaaa ggagattctc cagaggctcg agccttggcc aaacaggtgg
     2221 ccacggccct gcagaacctg cagaccaaaa ccaaccgggc tgtggccaac agcagaccgg
     2281 ccaaagcagc tgtacacctt gagggcaaga ttgagcaagc acagcggtgg attgataatc
     2341 ccacagtgga tgaccgtgga gtcggtcagg ctgccatccg ggggcttgtg gccgaagggc
     2401 atcgtctggc taatgttatg atggggcctt atcggcaaga tcttctcgcc aagtgtgacc
     2461 gagtggacca gctgacagcc cagctggctg acctggctgc cagaggggaa ggggagagtc
     2521 ctcaggcacg agcacttgca tctcagctcc aagactcctt aaaggatcta aaagctcgga
     2581 tgcaggaggc catgactcag gaagtgtcag atgttttcag cgataccaca actcccatca
     2641 agctgttggc agtggcagcc acggcgcctc ctgatgcgcc taacagggaa gaggtatttg
     2701 atgagagggc agctaacttt gaaaaccatt caggaaagct tggtgctacg gccgagaagg
     2761 cggctgcggt tggtactgct aataaatcaa cagtggaagg cattcaggcc tcagtgaaga
     2821 cggcccgaga actcacaccc caggtggtct cggctgctcg tatcttactt aggaaccctg
     2881 gaaatcaagc tgcttatgaa cattttgaga ccatgaagaa ccagtggatc gataatgttg
     2941 aaaaaatgac agggctggtg gacgaagcca ttgataccaa atctctgttg gatgcttcag
     3001 aagaagcaat taaaaaagac ctggacaagt gcaaggtagc tatggccaac attcagcctc
     3061 agatgctggt tgctggggca accagtattg ctcgtcgggc caaccggatc ctgctggtgg
     3121 ctaagaggga ggtggagaat tccgaggatc ccaagttccg tgaggctgtg aaagctgcct
     3181 ctgatgaatt gagcaaaacc atctccccga tggtgatgga tgcaaaagct gtggctggaa
     3241 acatttccga ccctggactg caaaagagct tcctggactc aggatatcgg atcctgggag
     3301 ctgtggccaa ggtcagagaa gccttccaac ctcaggagcc tgacttcccg ccgcctccac
     3361 cagaccttga acaactccga ctaacagatg agcttgctcc tcccaaacca cctctgcctg
     3421 aaggtgaggt ccctccacct aggcctccac caccagagga aaaggatgaa gagttccctg
     3481 agcagaaggc cggggaggtg attaaccagc caatgatgat ggctgccaga cagctccatg
     3541 atgaagctcg caaatggtcc agcaagggca atgacatcat tgcagcagcc aagcgcatgg
     3601 ctctgctgat ggctgagatg tctcggctgg taagaggggg cagtggtacc aagcgggcac
     3661 tcattcagtg tgccaaggac atcgccaagg cctcagatga ggtgactcgg ttggccaagg
     3721 aggttgccaa gcagtgcaca gataaacgga ttagaaccaa cctcttacag gtatgtgagc
     3781 gaatcccaac cataagcacc cagctcaaaa tcctgtccac agtgaaggcc accatgctgg
     3841 gccggaccaa catcagtgat gaggagtctg agcaggccac agagatgctg gttcacaatg
     3901 cccagaacct catgcagtct gtgaaggaga ctgtgcggga agctgaagct gcttcaatca
     3961 aaattcgaac agatgctgga tttacactgc gctgggttag aaagactccc tggtaccagt
     4021 aggcggccgc gactctagat cataatcagc cataccacat t
//
