LOCUS       sequence-85859-e        2550 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 433, Emission: 475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Markwardt ML, Kremers GJ, Kraft CA, Ray K, Cranfill PJ, Wilson KA, 
            Day RN, Wachter RM, Davidson MW, Rizzo MA
  TITLE     An improved cerulean fluorescent protein with enhanced brightness 
            and reduced reversible photoswitching.
  JOURNAL   PLoS One. 2011  . 6(3):e17896.
  PUBMED    21479270
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2011  . 6(3):e17896."
COMMENT     Sequence Label: mCerulean3-Zyxin-6
FEATURES             Location/Qualifiers
     source          1..2550
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      55..64
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1785..1794
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1791..1793,1794..1796,1797..2510)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIK
                     ANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1836..1857)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2097..2116)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2444..2465
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgct accggactca gatctcgagc tcaagcttcg aattccatgg
       61 cggccccccg cccgtctccc gcgatccccg tttcggtctc ggctccggct ttttacgccc
      121 cgcagaagaa gttcggccct gtggtggccc caaagcccaa agtgaatccc ttccggccca
      181 gggacagcga gcctcccccg gcacccgggg cccagcgcgc acagatgggc cgggtgggcg
      241 atattccccc gccgcccccg gaagactttc ccctgcctcc acctcccctt gctggggatg
      301 gcgacgatgc agagggtgct ctgggaggtg ccttcccgcc gccccctccc ccgatcgagg
      361 aatcatttcc ccctgcgcct ctggaggagg agatcttccc ttccccgccg cctcctccgg
      421 aggaggaggg agggcctgag gcccccatac cgcccccacc acagcccagg gagaaggtga
      481 gcagtattga tttggagatc gactctctgt cctcactgct ggatgacatg accaagaatg
      541 atcctttcaa agcccgggtg tcatctggat atgtgccccc accagtggcc actccattca
      601 gttccaagtc cagtaccaag cctgcagccg ggggcacagc acccctgcct ccttggaagt
      661 ccccttccag ctcccagcct ctgccccagg ttccggctcc ggctcagagc cagacacagt
      721 tccatgttca gccccagccc cagcccaagc ctcaggtcca actccatgtc cagtcccaga
      781 cccagcctgt gtctttggct aacacccagc cccgagggcc cccagcctca tctccggctc
      841 cagcccctaa gttttctcca gtgactccta agtttactcc tgtggcttcc aagttcagtc
      901 ctggagcccc aggtggatct gggtcacaac caaatcaaaa attggggcac cccgaagctc
      961 tttctgctgg cacaggctcc cctcaacctc ccagcttcac ctatgcccag cagagggaga
     1021 agccccgagt gcaggagaag cagcaccccg tgcccccacc ggctcagaac caaaaccagg
     1081 tgcgctcccc tggggcccca gggcccctga ctctgaagga ggtggaggag ctggagcagc
     1141 tgacccagca gctaatgcag gacatggagc atcctcagag gcagaatgtg gctgtcaacg
     1201 aactctgcgg ccgatgccat caacccctgg cccgggcgca gccagccgtc cgcgctctag
     1261 ggcagctgtt ccacatcgcc tgcttcacct gccaccagtg tgcgcagcag ctccagggcc
     1321 agcagttcta cagtctggag ggggcgccgt actgcgaggg ctgttacact gacaccctgg
     1381 agaagtgtaa cacctgcggg gagcccatca ctgaccgcat gctgagggcc acgggcaagg
     1441 cctatcaccc gcactgcttc acctgtgtgg tctgcgcccg ccccctggag ggcacctcct
     1501 tcatcgtgga ccaggccaac cggccccact gtgtccccga ctaccacaag cagtacgccc
     1561 cgaggtgctc cgtctgctct gagcccatca tgcctgagcc tggccgagat gagactgtgc
     1621 gagtggtcgc cctggacaag aacttccaca tgaagtgtta caagtgtgag gactgcggga
     1681 agcccctgtc gattgaggca gatgacaatg gctgcttccc cctggacggt cacgtgctct
     1741 gtcggaagtg ccacactgct agagcccaga cggatccacc ggtcgccacc atggtgagca
     1801 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     1861 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     1921 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     1981 ccctgagctg gggcgtgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact
     2041 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     2101 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     2161 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     2221 acaacgccat ccacggcaac gtctatatca ccgccgacaa gcagaagaac ggcatcaagg
     2281 ccaacttcgg cctcaactgc aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     2341 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     2401 cccagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     2461 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa agcggccgcg
     2521 actctagatc ataatcagcc ataccacatt
//
