LOCUS       sequence-85864-e        1934 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 433, Emission: 475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1934)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 1934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CYPet-Actin-3
FEATURES             Location/Qualifiers
     source          1..1934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(30..32,33..35,36..746)
                     /codon_start=1
                     /product="cyan fluorescent protein optimized for FRET with 
                     YPet (Nguyen and Daugherty, 2005)"
                     /label=CyPet
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFGGIVPILVELEGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSVMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKARHNITDGSVQLADHYQQNTPIGDGPVILPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(336..355)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 acgtcagatc cgctagcgct accggtacca tggtgagcaa gggagaggaa ctgttcggcg
       61 gcatcgtgcc catcctggtg gagctggagg gcgacgtgaa cggccacaag ttcagcgtga
      121 gcggcgaggg cgagggcgac gccacctacg gcaagctgac cctgaagttc atctgcacca
      181 ccggcaagct gcccgtgccc tggcccaccc tggtgaccac cctgacctgg ggcgtgcagt
      241 gcttcagccg gtaccccgac cacatgaagc agcacgactt cttcaagagc gtgatgcccg
      301 agggctacgt gcaggagcgg accatcttct tcaaggacga cggcaactac aagacccggg
      361 ccgaggtgaa gttcgagggc gacaccctgg tgaaccggat cgagctgaag ggcatcgact
      421 tcaaggagga cggcaacatc ctgggccaca agctggagta caactacatc agccacaacg
      481 tgtacatcac cgccgacaag cagaagaacg gcatcaaggc caacttcaag gcccggcaca
      541 acatcaccga cggcagcgtg cagctggccg accactacca gcagaacacc cccatcggcg
      601 acggccccgt gatcctgccc gacaaccact acctgagcac ccagagcgcc ctgagcaagg
      661 accccaacga gaagcgggac cacatggtgc tgctggagtt cgtgaccgcc gccggcatca
      721 cccacggcat ggacgaactg tacaaatctc gagctatgga tgatgatatc gccgcgctcg
      781 tcgtcgacaa cggctccggc atgtgcaagg ccggcttcgc gggcgacgat gccccccggg
      841 ccgtcttccc ctccatcgtg gggcgcccca ggcaccaggg cgtgatggtg ggcatgggtc
      901 agaaggattc ctatgtgggc gacgaggccc agagcaagag aggcatcctc accctgaagt
      961 accccatcga gcacggcatc gtcaccaact gggacgacat ggagaaaatc tggcaccaca
     1021 ccttctacaa tgagctgcgt gtggctcccg aggagcaccc cgtgctgctg accgaggccc
     1081 ccctgaaccc caaggccaac cgcgagaaga tgacccagat catgtttgag accttcaaca
     1141 ccccagccat gtacgttgct atccaggctg tgctatccct gtacgcctct ggccgtacca
     1201 ctggcatcgt gatggactcc ggtgacgggg tcacccacac tgtgcccatc tacgaggggt
     1261 atgccctccc ccatgccatc ctgcgtctgg acctggctgg ccgggacctg actgactacc
     1321 tcatgaagat cctcaccgag cgcggctaca gcttcaccac cacggccgag cgggaaatcg
     1381 tgcgtgacat taaggagaag ctgtgctacg tcgccctgga cttcgagcaa gagatggcca
     1441 cggctgcttc cagctcctcc ctggagaaga gctacgagct gcctgacggc caggtcatca
     1501 ccattggcaa tgagcggttc cgctgccctg aggcactctt ccagccttcc ttcctgggca
     1561 tggagtcctg tggcatccac gaaactacct tcaactccat catgaagtgt gacgtggaca
     1621 tccgcaaaga cctgtacgcc aacacagtgc tgtctggcgg caccaccatg taccctggca
     1681 ttgccgacag gatgcagaag gagatcactg ccctggcacc cagcacaatg aagatcaaga
     1741 tcattgctcc tcctgagcgc aagtactccg tgtggatcgg cggctccatc ctggcctcgc
     1801 tgtccacctt ccagcagatg tggatcagca agcaggagta tgacgagtcc ggcccctcca
     1861 tcgtccaccg caaatgcttc tagggatcca ccggatctag ataactgatc ataatcagcc
     1921 ataccacatt gaga
//
