LOCUS       sequence-85865-e         996 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 433, Emission: 475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 996)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CYPet-CytERM-N-17
FEATURES             Location/Qualifiers
     source          1..996
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      218..227
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(224..226,227..229,230..943)
                     /codon_start=1
                     /product="cyan fluorescent protein optimized for FRET with 
                     YPet (Nguyen and Daugherty, 2005)"
                     /label=CyPet
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFGGIVPILVELEGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSVMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKARHNITDGSVQLADHYQQNTPIGDGPVILPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(530..549)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ttagtgaacc gtcagatccg ctagcgctac cggactcaga tctggtaccc ttgcagcttc
       61 cagttgtcag gaagagaagg ctcccatgga tcctgtggtg gtgctggggc tctgtctctc
      121 ctgtttgctt ctcctttcac tctggaaaca gagctatggg ggagggaagc ttcgaattct
      181 gcagtcgacg gtaccgcggg cccgggatcc accggtcgcc accatggtga gcaagggaga
      241 ggaactgttc ggcggcatcg tgcccatcct ggtggagctg gagggcgacg tgaacggcca
      301 caagttcagc gtgagcggcg agggcgaggg cgacgccacc tacggcaagc tgaccctgaa
      361 gttcatctgc accaccggca agctgcccgt gccctggccc accctggtga ccaccctgac
      421 ctggggcgtg cagtgcttca gccggtaccc cgaccacatg aagcagcacg acttcttcaa
      481 gagcgtgatg cccgagggct acgtgcagga gcggaccatc ttcttcaagg acgacggcaa
      541 ctacaagacc cgggccgagg tgaagttcga gggcgacacc ctggtgaacc ggatcgagct
      601 gaagggcatc gacttcaagg aggacggcaa catcctgggc cacaagctgg agtacaacta
      661 catcagccac aacgtgtaca tcaccgccga caagcagaag aacggcatca aggccaactt
      721 caaggcccgg cacaacatca ccgacggcag cgtgcagctg gccgaccact accagcagaa
      781 cacccccatc ggcgacggcc ccgtgatcct gcccgacaac cactacctga gcacccagag
      841 cgccctgagc aaggacccca acgagaagcg ggaccacatg gtgctgctgg agttcgtgac
      901 cgccgccggc atcacccacg gcatggacga actgtacaaa taaagcggcc gcgactctag
      961 atcataatca gccataccac attgtagagg ttgccc
//
