LOCUS       sequence-85867-e        1935 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 462, Emission: 492.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Shaner NC, Patterson GH, Davidson MW
  TITLE     Advances in fluorescent protein technology
  JOURNAL   J Cell Sci. 2007 Dec 15;120(Pt 24):4247-60
  PUBMED    18057027
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2007-12-15"; volume: "120(Pt 24)"; pages: "4247-6"
COMMENT     Sequence Label: mTFP1-Actin-7
FEATURES             Location/Qualifiers
     source          1..1935
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             32..739
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
ORIGIN
        1 gtcagatccg ctagcgctac cggtcgccac catggtgagc aagggcgagg agaccacaat
       61 gggcgtaatc aagcccgaca tgaagatcaa gctgaagatg gagggcaacg tgaatggcca
      121 cgccttcgtg atcgagggcg agggcgaggg caagccctac gacggcacca acaccatcaa
      181 cctggaggtg aaggagggag cccccctgcc cttctcctac gacattctga ccaccgcgtt
      241 cgcctacggc aacagggcct tcaccaagta ccccgacgac atccccaact acttcaagca
      301 gtccttcccc gagggctact cttgggagcg caccatgacc ttcgaggaca agggcatcgt
      361 gaaggtgaag tccgacatct ccatggagga ggactccttc atctacgaga tacacctcaa
      421 gggcgagaac ttccccccca acggccccgt gatgcagaag aagaccaccg gctgggacgc
      481 ctccaccgag aggatgtacg tgcgcgacgg cgtgctgaag ggcgacgtca agcacaagct
      541 gctgctggag ggcggcggcc accaccgcgt tgacttcaag accatctaca gggccaagaa
      601 ggcggtgaag ctgcccgact atcactttgt ggaccaccgc atcgagatcc tgaaccacga
      661 caaggactac aacaaggtga ccgtttacga gagcgccgtg gcccgcaact ccaccgacgg
      721 catggacgag ctgtacaagt ccggactcag atctcgagct atggatgatg atatcgccgc
      781 gctcgtcgtc gacaacggct ccggcatgtg caaggccggc ttcgcgggcg acgatgcccc
      841 ccgggccgtc ttcccctcca tcgtggggcg ccccaggcac cagggcgtga tggtgggcat
      901 gggtcagaag gattcctatg tgggcgacga ggcccagagc aagagaggca tcctcaccct
      961 gaagtacccc atcgagcacg gcatcgtcac caactgggac gacatggaga aaatctggca
     1021 ccacaccttc tacaatgagc tgcgtgtggc tcccgaggag caccccgtgc tgctgaccga
     1081 ggcccccctg aaccccaagg ccaaccgcga gaagatgacc cagatcatgt ttgagacctt
     1141 caacacccca gccatgtacg ttgctatcca ggctgtgcta tccctgtacg cctctggccg
     1201 taccactggc atcgtgatgg actccggtga cggggtcacc cacactgtgc ccatctacga
     1261 ggggtatgcc ctcccccatg ccatcctgcg tctggacctg gctggccggg acctgactga
     1321 ctacctcatg aagatcctca ccgagcgcgg ctacagcttc accaccacgg ccgagcggga
     1381 aatcgtgcgt gacattaagg agaagctgtg ctacgtcgcc ctggacttcg agcaagagat
     1441 ggccacggct gcttccagct cctccctgga gaagagctac gagctgcctg acggccaggt
     1501 catcaccatt ggcaatgagc ggttccgctg ccctgaggca ctcttccagc cttccttcct
     1561 gggcatggag tcctgtggca tccacgaaac taccttcaac tccatcatga agtgtgacgt
     1621 ggacatccgc aaagacctgt acgccaacac agtgctgtct ggcggcacca ccatgtaccc
     1681 tggcattgcc gacaggatgc agaaggagat cactgccctg gcacccagca caatgaagat
     1741 caagatcatt gctcctcctg agcgcaagta ctccgtgtgg atcggcggct ccatcctggc
     1801 ctcgctgtcc accttccagc agatgtggat cagcaagcag gagtatgacg agtccggccc
     1861 ctccatcgtc caccgcaaat gcttctaggg atccaccgga tctagataac tgatcataat
     1921 cagccatacc acatt
//
