LOCUS       sequence-85873-e        1828 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Lysosomes, Excitation: 462, Emission: 492.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Ai HW, Olenych SG, Wong P, Davidson MW, Campbell RE
  TITLE     Hue-shifted monomeric variants of Clavularia cyan fluorescent 
            protein: identification of the molecular determinants of color and 
            applications in fluorescence imaging.
  JOURNAL   BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/1741-7007-6-13.
  PUBMED    18325109
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2008-03-6"; volume: "6:13. doi"; pages: " 
            10.1186/1741-7007-6-13"
COMMENT     Sequence Label: mTFP1-CathespinB-6
FEATURES             Location/Qualifiers
     source          1..1828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1073..1082
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1079..1789
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
ORIGIN
        1 tgacgtcaga tccgctagcg ctaccggact cagatctcga gctcaagctt cgaattccag
       61 gatgtggtgg tccttgatcc ctctctcttg cctgctggca ctgaccagtg cccatgacaa
      121 gccttccttt cacccactgt cggacgacat gattaactat atcaacaaac agaatacaac
      181 atggcaggct ggacgcaact tctacaatgt tgacataagc tatctgaaga agctgtgtgg
      241 aactgtcctg ggtggaccca agctgccgga aagggttggg ttcagcgagg acataaatct
      301 acctgaatcc tttgatgcac gggaacagtg gtccaattgc ccgaccatcg cacagatcag
      361 agaccagggg tcctgtggct cttgttgggc atttggggca gtggaagcca tgtctgaccg
      421 aatctgcatt cacaccaatg gccgagtcaa tgtggaggtg tctgctgagg acctgcttac
      481 ctgctgtggt atccagtgtg gggatggctg taatggtggc tatccctctg gagcatggaa
      541 cttctggact agaaaaggcc tggtttctgg tggagtatac aattctcata taggctgctt
      601 accctacacc atccctccct gtgaacacca tgtcaatggc tcccgtcccc catgcactgg
      661 agaaggagat actcccaagt gcaacaagat gtgtgaggct ggctactcca catcctacaa
      721 ggaagataag cactatgggt acacttccta cagtgtgtct gacagcgaga aggagatcat
      781 ggcggaaatc tacaaaaatg gcccagtgga gggtgctttt actgtgtttt ctgacttctt
      841 gacttacaaa tcaggcgtat acaagcatga agccggtgat gtgatgggag gccatgccat
      901 ccgcattctg ggctggggaa tagagaatgg agtaccctac tggctggtag caaactcctg
      961 gaacgttgac tggggtgata atggtttctt taaaatcctc agaggagaga accactgtgg
     1021 aattgaatca gaaatcgtgg ctggaatccc acgcactcag gatccaccgg tcgccaccat
     1081 ggtgagcaag ggcgaggaga ccacaatggg cgtaatcaag cccgacatga agatcaagct
     1141 gaagatggag ggcaacgtga atggccacgc cttcgtgatc gagggcgagg gcgagggcaa
     1201 gccctacgac ggcaccaaca ccatcaacct ggaggtgaag gagggagccc ccctgccctt
     1261 ctcctacgac attctgacca ccgcgttcgc ctacggcaac agggccttca ccaagtaccc
     1321 cgacgacatc cccaactact tcaagcagtc cttccccgag ggctactctt gggagcgcac
     1381 catgaccttc gaggacaagg gcatcgtgaa ggtgaagtcc gacatctcca tggaggagga
     1441 ctccttcatc tacgagatac acctcaaggg cgagaacttc ccccccaacg gccccgtgat
     1501 gcagaagaag accaccggct gggacgcctc caccgagagg atgtacgtgc gcgacggcgt
     1561 gctgaagggc gacgtcaagc acaagctgct gctggagggc ggcggccacc accgcgttga
     1621 cttcaagacc atctacaggg ccaagaaggc ggtgaagctg cccgactatc actttgtgga
     1681 ccaccgcatc gagatcctga accacgacaa ggactacaac aaggtgaccg tttacgagag
     1741 cgccgtggcc cgcaactcca ccgacggcat ggacgagctg tacaagtaag cggccgcgac
     1801 tctagatcat aatcagccat accacatt
//
