LOCUS       sequence-85875-e        1167 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus, Excitation: 462, Emission: 492.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Ai HW, Olenych SG, Wong P, Davidson MW, Campbell RE
  TITLE     Hue-shifted monomeric variants of Clavularia cyan fluorescent 
            protein: identification of the molecular determinants of color and 
            applications in fluorescence imaging.
  JOURNAL   BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/1741-7007-6-13.
  PUBMED    18325109
REFERENCE   2  (bases 1 to 1167)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2008-03-6"; volume: "6:13. doi"; pages: " 
            10.1186/1741-7007-6-13"
COMMENT     Sequence Label: mTFP1-CEBPA237-C-4
FEATURES             Location/Qualifiers
     source          1..1167
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      21..30
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             27..734
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
ORIGIN
        1 atccgctagc gctaccggtc gccaccatgg tgagcaaggg cgaggagacc acaatgggcg
       61 taatcaagcc cgacatgaag atcaagctga agatggaggg caacgtgaat ggccacgcct
      121 tcgtgatcga gggcgagggc gagggcaagc cctacgacgg caccaacacc atcaacctgg
      181 aggtgaagga gggagccccc ctgcccttct cctacgacat tctgaccacc gcgttcgcct
      241 acggcaacag ggccttcacc aagtaccccg acgacatccc caactacttc aagcagtcct
      301 tccccgaggg ctactcttgg gagcgcacca tgaccttcga ggacaagggc atcgtgaagg
      361 tgaagtccga catctccatg gaggaggact ccttcatcta cgagatacac ctcaagggcg
      421 agaacttccc ccccaacggc cccgtgatgc agaagaagac caccggctgg gacgcctcca
      481 ccgagaggat gtacgtgcgc gacggcgtgc tgaagggcga cgtcaagcac aagctgctgc
      541 tggagggcgg cggccaccac cgcgttgact tcaagaccat ctacagggcc aagaaggcgg
      601 tgaagctgcc cgactatcac tttgtggacc accgcatcga gatcctgaac cacgacaagg
      661 actacaacaa ggtgaccgtt tacgagagcg ccgtggcccg caactccacc gacggcatgg
      721 acgagctgta caagtccgga cctgcaatgg gtgctgcggg cctgccgggc cccgggggct
      781 cgctcaaggg cttggctggt ccgcaccccg acctccgcac cggcggcggc ggcggcggcg
      841 gggccggcgc gggcaaggcc aagaagtcgg tggataagaa cagcaacgag taccgggtac
      901 ggcgggaacg caacaacatc gcggtgcgca agagccgaga taaagccaaa cagcgcaacg
      961 tggagacgca gcagaaggtg ttggagttga ccagtgacaa tgaccgcctg cgcaagcggg
     1021 tggaacagct gagccgtgaa ctggacacgc tgcggggtat cttccgccag ctgcctgaga
     1081 gctccttggt caaggccatg ggcaactgcg cgtgaggcgc gcggatccac cggatctaga
     1141 taactgatca taatcagcca taccaca
//
