LOCUS       sequence-85876-e        1349 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Centromeres, Excitation: 462, Emission: 492.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Ai HW, Olenych SG, Wong P, Davidson MW, Campbell RE
  TITLE     Hue-shifted monomeric variants of Clavularia cyan fluorescent 
            protein: identification of the molecular determinants of color and 
            applications in fluorescence imaging.
  JOURNAL   BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/1741-7007-6-13.
  PUBMED    18325109
REFERENCE   2  (bases 1 to 1349)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2008-03-6"; volume: "6:13. doi"; pages: " 
            10.1186/1741-7007-6-13"
COMMENT     Sequence Label: mTFP1-CENPB-N-22
FEATURES             Location/Qualifiers
     source          1..1349
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      21..30
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    534..586
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      594..603
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             600..1310
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
ORIGIN
        1 ctgcgacgtc agatccgcta gccaccatgg gccccaagcg gcggcagctg acgttccggg
       61 agaagtcgcg gatcatccag gaggtggagg agaacccgga cctacgcaag ggcgagatcg
      121 cgcggcgctt caacatcccg ccgtccacgc tgagcacgat cctgaagaac aagcgcgcca
      181 tcctggcgtc ggagcgcaag tacggggtgg cctccacctg ccgcaagacc aacaagctgt
      241 ctccctacga caagctcgag ggcttgctca tcgcctggtt ccagcagatc cgcgccgccg
      301 gcctgccggt caagggcatc atcctcaagg agaaggcgct gcgcatagcc gaggagctgg
      361 gcatggacga cttcaccgcc tccaacggct ggctggaccg cttccgccgg cgccacggcg
      421 tggtgtcctg cagcggcgtg gcccgcgccc gcgcgcgaaa cgctgccccc cgcaccccgg
      481 cggcgcctgc cagtccggcc gcggtgccct cggagggcag tggcgggagc actctcgagc
      541 tcaagcttcg aattctgcag tcgacggtac cgcgggcccg ggatccaccg gtcgccacca
      601 tggtgagcaa gggcgaggag accacaatgg gcgtaatcaa gcccgacatg aagatcaagc
      661 tgaagatgga gggcaacgtg aatggccacg ccttcgtgat cgagggcgag ggcgagggca
      721 agccctacga cggcaccaac accatcaacc tggaggtgaa ggagggagcc cccctgccct
      781 tctcctacga cattctgacc accgcgttcg cctacggcaa cagggccttc accaagtacc
      841 ccgacgacat ccccaactac ttcaagcagt ccttccccga gggctactct tgggagcgca
      901 ccatgacctt cgaggacaag ggcatcgtga aggtgaagtc cgacatctcc atggaggagg
      961 actccttcat ctacgagata cacctcaagg gcgagaactt cccccccaac ggccccgtga
     1021 tgcagaagaa gaccaccggc tgggacgcct ccaccgagag gatgtacgtg cgcgacggcg
     1081 tgctgaaggg cgacgtcaag cacaagctgc tgctggaggg cggcggccac caccgcgttg
     1141 acttcaagac catctacagg gccaagaagg cggtgaagct gcccgactat cactttgtgg
     1201 accaccgcat cgagatcctg aaccacgaca aggactacaa caaggtgacc gtttacgaga
     1261 gcgccgtggc ccgcaactcc accgacggca tggacgagct gtacaagtaa gcggccgcga
     1321 ctctagatca taatcagcca taccacatt
//
