LOCUS       sequence-85877-e        1470 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Clathrin Vesicles, Excitation: 462, Emission: 492.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Ai HW, Olenych SG, Wong P, Davidson MW, Campbell RE
  TITLE     Hue-shifted monomeric variants of Clavularia cyan fluorescent 
            protein: identification of the molecular determinants of color and 
            applications in fluorescence imaging.
  JOURNAL   BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/1741-7007-6-13.
  PUBMED    18325109
REFERENCE   2  (bases 1 to 1470)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2008-03-6"; volume: "6:13. doi"; pages: " 
            10.1186/1741-7007-6-13"
COMMENT     Sequence Label: mTFP1-Clathrin-15
FEATURES             Location/Qualifiers
     source          1..1470
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             35..742
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggagaccac
       61 aatgggcgta atcaagcccg acatgaagat caagctgaag atggagggca acgtgaatgg
      121 ccacgccttc gtgatcgagg gcgagggcga gggcaagccc tacgacggca ccaacaccat
      181 caacctggag gtgaaggagg gagcccccct gcccttctcc tacgacattc tgaccaccgc
      241 gttcgcctac ggcaacaggg ccttcaccaa gtaccccgac gacatcccca actacttcaa
      301 gcagtccttc cccgagggct actcttggga gcgcaccatg accttcgagg acaagggcat
      361 cgtgaaggtg aagtccgaca tctccatgga ggaggactcc ttcatctacg agatacacct
      421 caagggcgag aacttccccc ccaacggccc cgtgatgcag aagaagacca ccggctggga
      481 cgcctccacc gagaggatgt acgtgcgcga cggcgtgctg aagggcgacg tcaagcacaa
      541 gctgctgctg gagggcggcg gccaccaccg cgttgacttc aagaccatct acagggccaa
      601 gaaggcggtg aagctgcccg actatcactt tgtggaccac cgcatcgaga tcctgaacca
      661 cgacaaggac tacaacaagg tgaccgttta cgagagcgcc gtggcccgca actccaccga
      721 cggcatggac gagctgtaca agtccggact cagatctcga gctcaagctt cgaattctgc
      781 agtcgacatg gctgatgact ttggcttctt ctcgtcgtcg gagagtggtg ccccggaggc
      841 ggcggaggag gacccggcgg ccgccttcct ggcccagcag gagagcgaga ttgcaggcat
      901 agagaacgac gagggcttcg gggcacctgc cggcagccat gcggcccccg cacagccggg
      961 ccccacgagt ggggctggtt ctgaggacat ggggaccaca gtcaatggag atgtgtttca
     1021 ggaggccaac ggtcctgctg atggctacgc agccattgcc caggctgaca ggctgaccca
     1081 ggagcctgag agcatccgca agtggcgaga ggagcagagg aaacggctgc aagagctgga
     1141 tgctgcatct aaggtcacgg aacaggaatg gcgggagaag gccaagaagg acctggagga
     1201 gtggaaccag cgccagagtg aacaagtaga gaagaacaag atcaacaacc gggcatccga
     1261 ggaggctttc gtgaaggaat ccaaggagga gaccccaggc acagagtggg agaaggtggc
     1321 ccagctatgt gacttcaacc ccaagagcag caagcagtgc aaagatgtgt cccgcctgcg
     1381 ctcggtgctc atgtccctga agcagacgcc actgtcccgc tagggatcca ccggatctag
     1441 ataactgatc ataatcagcc ataccacatt
//
