LOCUS       sequence-85878-e        2425 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane/Vesicles, Excitation: 462, Emission: 492.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Ai HW, Olenych SG, Wong P, Davidson MW, Campbell RE
  TITLE     Hue-shifted monomeric variants of Clavularia cyan fluorescent 
            protein: identification of the molecular determinants of color and 
            applications in fluorescence imaging.
  JOURNAL   BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/1741-7007-6-13.
  PUBMED    18325109
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2008-03-6"; volume: "6:13. doi"; pages: " 
            10.1186/1741-7007-6-13"
COMMENT     Sequence Label: mTFP1-C-Src-7
FEATURES             Location/Qualifiers
     source          1..2425
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             56..97
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     regulatory      1670..1679
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1676..2386
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
ORIGIN
        1 acgtcagatc cgctagcgct accggactca gatctcgagc tcaagcttcg aattcatggg
       61 gagcagcaag agcaagccca aggaccccag ccagcgccgg cgcagcctgg agccacccga
      121 cagcacccac cacgggggat tcccagcctc gcagaccccc aacaagacag cagcccccga
      181 cacgcaccgc acccccagcc gctcctttgg gaccgtggcc accgagccca agctcttcgg
      241 gggcttcaac acttctgaca ccgtcacgtc gccgcagcgt gccggggcac tggctggcgg
      301 cgtcaccact ttcgtggctc tctatgacta cgagtcccgg actgaaacgg acttgtcctt
      361 caagaaagga gaacgcctgc agattgtcaa caacacggaa ggtgactggt ggctggctca
      421 ttccctcact acaggacaga cgggctacat ccccagtaac tatgtcgcgc cctcagactc
      481 catccaggct gaagagtggt actttgggaa gatcactcgt cgggagtccg agcggctgct
      541 gctcaacccc gaaaaccccc ggggaacctt cttggtccgg gagagcgaga cgacaaaagg
      601 tgcctattgc ctctccgttt ctgactttga caacgccaag gggctcaatg tgaagcacta
      661 caagatccgc aagctggaca gcggcggctt ctacatcacc tcacgcacac agttcagcag
      721 cctgcagcag ctggtggcct actactccaa acatgctgat ggcttgtgcc accgcctgac
      781 caacgtctgc cccacgtcca agccccagac ccagggactc gccaaggacg cgtgggaaat
      841 cccccgggag tcgctgcggc tggaggtgaa gctggggcag ggctgctttg gagaggtctg
      901 gatggggacc tggaacggca ccaccagagt ggccataaag actctgaagc ccggcaccat
      961 gtccccggag gccttcctgc aggaagccca agtgatgaag aagctccggc atgagaagct
     1021 ggttcagctg tacgcagtgg tgtcggaaga gcccatctac atcgtcactg agtacatgag
     1081 caaggggagc ctcctggatt tcctgaaggg agagatgggc aagtacctgc ggctgccaca
     1141 gctcgtcgat atggctgctc agattgcatc cggcatggcc tatgtggaga ggatgaacta
     1201 cgtgcaccga gacctgcggg cggccaacat cctggtgggg gagaacctgg tgtgcaaggt
     1261 ggctgacttt gggctggcac gcctcatcga ggacaacgag tacacagcac ggcaaggtgc
     1321 caagttcccc atcaagtgga cagcccccga ggcagccctc tatggccggt tcaccatcaa
     1381 gtcggatgtc tggtccttcg gcatcctgct gactgagctg accaccaagg gccgggtgcc
     1441 atacccaggg atggtcaaca gggaggtgct ggaccaggtg gagaggggct accgcatgcc
     1501 ctgcccgccc gagtgccccg agtcgctgca tgacctcatg tgccagtgct ggcggaagga
     1561 ccctgaggag cggcccactt ttgagtacct gcaggccttc ctggaggact acttcacctc
     1621 gacagagccc cagtaccagc ctggagagaa cctacgggat ccaccggtcg ccaccatggt
     1681 gagcaagggc gaggagacca caatgggcgt aatcaagccc gacatgaaga tcaagctgaa
     1741 gatggagggc aacgtgaatg gccacgcctt cgtgatcgag ggcgagggcg agggcaagcc
     1801 ctacgacggc accaacacca tcaacctgga ggtgaaggag ggagcccccc tgcccttctc
     1861 ctacgacatt ctgaccaccg cgttcgccta cggcaacagg gccttcacca agtaccccga
     1921 cgacatcccc aactacttca agcagtcctt ccccgagggc tactcttggg agcgcaccat
     1981 gaccttcgag gacaagggca tcgtgaaggt gaagtccgac atctccatgg aggaggactc
     2041 cttcatctac gagatacacc tcaagggcga gaacttcccc cccaacggcc ccgtgatgca
     2101 gaagaagacc accggctggg acgcctccac cgagaggatg tacgtgcgcg acggcgtgct
     2161 gaagggcgac gtcaagcaca agctgctgct ggagggcggc ggccaccacc gcgttgactt
     2221 caagaccatc tacagggcca agaaggcggt gaagctgccc gactatcact ttgtggacca
     2281 ccgcatcgag atcctgaacc acgacaagga ctacaacaag gtgaccgttt acgagagcgc
     2341 cgtggcccgc aactccaccg acggcatgga cgagctgtac aagtaagcgg ccgcgactct
     2401 agatcataat cagccatacc acatt
//
