LOCUS       sequence-85879-e        1555 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Gap Junctions, Excitation: 462, Emission: 492.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Ai HW, Olenych SG, Wong P, Davidson MW, Campbell RE
  TITLE     Hue-shifted monomeric variants of Clavularia cyan fluorescent 
            protein: identification of the molecular determinants of color and 
            applications in fluorescence imaging.
  JOURNAL   BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/1741-7007-6-13.
  PUBMED    18325109
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2008-03-6"; volume: "6:13. doi"; pages: " 
            10.1186/1741-7007-6-13"
COMMENT     Sequence Label: mTFP1-Cx26-7
FEATURES             Location/Qualifiers
     source          1..1555
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      799..808
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             805..1515
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
ORIGIN
        1 cgacgtcaga tccgctagcg ctaccggact cagatctcga gctcaagctt cgaattcgcg
       61 gccgcacgct cctcgggaca cagtgccaac catccagagg acaagatgga ttggggcaca
      121 ctacagagca tcctcggggg tgtcaacaag cactccacca gcattgggaa aatctggctc
      181 actgtcctct tcatcttccg catcatgatc ctcgtggtgg ccgcgaagga ggtgtgggga
      241 gatgagcaag ccgattttgt ttgcaacact ctccagcctg gctgtaagaa tgtgtgctac
      301 gaccactact tccccatctc tcacatccgg ctctgggctc tgcagctgat catggtgtcc
      361 acgccggccc tcctggtagc tatgcacgtg gcctaccgga gacacgaaaa gaaacggaag
      421 ttcatgaagg gagagataaa gaacgagttt aaggacatcg aagagatcaa aacccagaag
      481 gtccgtatcg aagggtccct gtggtggacc tacaccacca gcatcttctt ccgggtcatc
      541 ttcgaagctg tcttcatgta tgtcttttac atcatgtaca atggcttctt catgcagcgt
      601 ctggtgaagt gtaacgcctg gccttgtccc aatacagtgg actgcttcat ttccaggccc
      661 acagaaaaga ctgtcttcac ggtgttcatg atctctgtgt ctggaatttg catcctgcta
      721 aacatcacag agctgtgcta tctgttcatt aggtattgct cagggaagtc caaaagacca
      781 gtcgcggatc caccggtcgc caccatggtg agcaagggcg aggagaccac aatgggcgta
      841 atcaagcccg acatgaagat caagctgaag atggagggca acgtgaatgg ccacgccttc
      901 gtgatcgagg gcgagggcga gggcaagccc tacgacggca ccaacaccat caacctggag
      961 gtgaaggagg gagcccccct gcccttctcc tacgacattc tgaccaccgc gttcgcctac
     1021 ggcaacaggg ccttcaccaa gtaccccgac gacatcccca actacttcaa gcagtccttc
     1081 cccgagggct actcttggga gcgcaccatg accttcgagg acaagggcat cgtgaaggtg
     1141 aagtccgaca tctccatgga ggaggactcc ttcatctacg agatacacct caagggcgag
     1201 aacttccccc ccaacggccc cgtgatgcag aagaagacca ccggctggga cgcctccacc
     1261 gagaggatgt acgtgcgcga cggcgtgctg aagggcgacg tcaagcacaa gctgctgctg
     1321 gagggcggcg gccaccaccg cgttgacttc aagaccatct acagggccaa gaaggcggtg
     1381 aagctgcccg actatcactt tgtggaccac cgcatcgaga tcctgaacca cgacaaggac
     1441 tacaacaagg tgaccgttta cgagagcgcc gtggcccgca actccaccga cggcatggac
     1501 gagctgtaca agtaagcggc cgcgactcta gatcataatc agccatacca cattg
//
