LOCUS       sequence-85882-e        1065 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 462, Emission: 492.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Ai HW, Olenych SG, Wong P, Davidson MW, Campbell RE
  TITLE     Hue-shifted monomeric variants of Clavularia cyan fluorescent 
            protein: identification of the molecular determinants of color and 
            applications in fluorescence imaging.
  JOURNAL   BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/1741-7007-6-13.
  PUBMED    18325109
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2008-03-6"; volume: "6:13. doi"; pages: " 
            10.1186/1741-7007-6-13"
COMMENT     Sequence Label: mTFP1-CytERM-N-17
FEATURES             Location/Qualifiers
     source          1..1065
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      259..268
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             265..975
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
ORIGIN
        1 aggcgtgtac ggtgggaggt ctataataag cagagctggt ttagtgaacc gtcagatccg
       61 ctagcgctac cggactcaga tctggtaccc cttgcagctt ccagttgtca ggaagagaag
      121 gctcccatgg atcctgtggt ggtgctgggg ctctgtctct cctgtttgct tctcctttca
      181 ctctggaaac agagctatgg gggagggaag cttcgaattc tgcagtcgac ggtaccgcgg
      241 gcccgggatc caccggtcgc caccatggtg agcaagggcg aggagaccac aatgggcgta
      301 atcaagcccg acatgaagat caagctgaag atggagggca acgtgaatgg ccacgccttc
      361 gtgatcgagg gcgagggcga gggcaagccc tacgacggca ccaacaccat caacctggag
      421 gtgaaggagg gagcccccct gcccttctcc tacgacattc tgaccaccgc gttcgcctac
      481 ggcaacaggg ccttcaccaa gtaccccgac gacatcccca actacttcaa gcagtccttc
      541 cccgagggct actcttggga gcgcaccatg accttcgagg acaagggcat cgtgaaggtg
      601 aagtccgaca tctccatgga ggaggactcc ttcatctacg agatacacct caagggcgag
      661 aacttccccc ccaacggccc cgtgatgcag aagaagacca ccggctggga cgcctccacc
      721 gagaggatgt acgtgcgcga cggcgtgctg aagggcgacg tcaagcacaa gctgctgctg
      781 gagggcggcg gccaccaccg cgttgacttc aagaccatct acagggccaa gaaggcggtg
      841 aagctgcccg actatcactt tgtggaccac cgcatcgaga tcctgaacca cgacaaggac
      901 tacaacaagg tgaccgttta cgagagcgcc gtggcccgca actccaccga cggcatggac
      961 gagctgtaca agtaagcggc cgcgactcta gatcataatc agccatacca catttgtaga
     1021 ggttttactt gctttaaaaa acctcccaca cctccccctg acctg
//
