LOCUS       sequence-85884-e        1491 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Golgi/ER, Excitation: 462, Emission: 492.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1491)
  AUTHORS   Ai HW, Olenych SG, Wong P, Davidson MW, Campbell RE
  TITLE     Hue-shifted monomeric variants of Clavularia cyan fluorescent 
            protein: identification of the molecular determinants of color and 
            applications in fluorescence imaging.
  JOURNAL   BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/1741-7007-6-13.
  PUBMED    18325109
REFERENCE   2  (bases 1 to 1491)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2008-03-6"; volume: "6:13. doi"; pages: " 
            10.1186/1741-7007-6-13"
COMMENT     Sequence Label: mTFP1-ELP-1-25
FEATURES             Location/Qualifiers
     source          1..1491
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      22..31
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      739..748
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             745..1455
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
ORIGIN
        1 gacgtcagat ccgctagcgc cgccaccatg gccatgaaca ttttccggct gactggggac
       61 ctgtcccacc tggcggccat cgtcatcctg ctgctgaaga tctggaagac gcgctcctgc
      121 gccggtattt ctgggaaaag ccagcttctg tttgcactgg tcttcacaac tcgttacctg
      181 gatcttttta cttcatttat ttcattgtat aacacatcta tgaaggttat ctaccttgcc
      241 tgctcctatg ccacagtgta cctgatctac ctgaaattta aggcaaccta cgatggaaat
      301 catgatacct tccgagtgga gtttctggtg gtccctgtgg gaggcctctc atttttagtt
      361 aatcacgatt tctctcctct tgagatcctc tggaccttct ccatctacct ggagtccgtg
      421 gctatccttc cgcagctgtt tatgatcagc aagactgggg aggccgagac catcaccacc
      481 cactacctgt tcttcctggg cctctatcgt gctttgtatc ttgtcaactg gatctggcgc
      541 ttctactttg agggcttctt tgacctcatt gctgtggtgg ccggcgtagt ccagaccatc
      601 ctatactgtg acttcttcta cttgtacatt acaaaagtac tcaagggaaa gaagctcagt
      661 ttgccagcag gcggtggggg ggagctcaag cttcgaattc tgcagtcgac ggtaccgcgg
      721 gcccgggatc caccggtcgc caccatggtg agcaagggcg aggagaccac aatgggcgta
      781 atcaagcccg acatgaagat caagctgaag atggagggca acgtgaatgg ccacgccttc
      841 gtgatcgagg gcgagggcga gggcaagccc tacgacggca ccaacaccat caacctggag
      901 gtgaaggagg gagcccccct gcccttctcc tacgacattc tgaccaccgc gttcgcctac
      961 ggcaacaggg ccttcaccaa gtaccccgac gacatcccca actacttcaa gcagtccttc
     1021 cccgagggct actcttggga gcgcaccatg accttcgagg acaagggcat cgtgaaggtg
     1081 aagtccgaca tctccatgga ggaggactcc ttcatctacg agatacacct caagggcgag
     1141 aacttccccc ccaacggccc cgtgatgcag aagaagacca ccggctggga cgcctccacc
     1201 gagaggatgt acgtgcgcga cggcgtgctg aagggcgacg tcaagcacaa gctgctgctg
     1261 gagggcggcg gccaccaccg cgttgacttc aagaccatct acagggccaa gaaggcggtg
     1321 aagctgcccg actatcactt tgtggaccac cgcatcgaga tcctgaacca cgacaaggac
     1381 tacaacaagg tgaccgttta cgagagcgcc gtggcccgca actccaccga cggcatggac
     1441 gagctgtaca agtaagcggc cgcgactcta gatcataatc agccatacca c
//
