LOCUS       sequence-85887-e         946 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Plasma Membrane, Excitation: 462, Emission: 492.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Ai HW, Olenych SG, Wong P, Davidson MW, Campbell RE
  TITLE     Hue-shifted monomeric variants of Clavularia cyan fluorescent 
            protein: identification of the molecular determinants of color and 
            applications in fluorescence imaging.
  JOURNAL   BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/1741-7007-6-13.
  PUBMED    18325109
REFERENCE   2  (bases 1 to 946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2008-03-6"; volume: "6:13. doi"; pages: " 
            10.1186/1741-7007-6-13"
COMMENT     Sequence Label: mTFP1-Farnesyl-5
FEATURES             Location/Qualifiers
     source          1..946
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      92..101
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             98..805
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
ORIGIN
        1 tcgcccatga cgcaatggcg gtagcgtgta cggtggagtc tatataagca gagctggtta
       61 gtgaccgtca gatccgctag cgctaccggt cgccaccatg gtgagcaagg gcgaggagac
      121 cacaatgggc gtaatcaagc ccgacatgaa gatcaagctg aagatggagg gcaacgtgaa
      181 tggccacgcc ttcgtgatcg agggcgaggg cgagggcaag ccctacgacg gcaccaacac
      241 catcaacctg gaggtgaagg agggagcccc cctgcccttc tcctacgaca ttctgaccac
      301 cgcgttcgcc tacggcaaca gggccttcac caagtacccc gacgacatcc ccaactactt
      361 caagcagtcc ttccccgagg gctactcttg ggagcgcacc atgaccttcg aggacaaggg
      421 catcgtgaag gtgaagtccg acatctccat ggaggaggac tccttcatct acgagataca
      481 cctcaagggc gagaacttcc cccccaacgg ccccgtgatg cagaagaaga ccaccggctg
      541 ggacgcctcc accgagagga tgtacgtgcg cgacggcgtg ctgaagggcg acgtcaagca
      601 caagctgctg ctggagggcg gcggccacca ccgcgttgac ttcaagacca tctacagggc
      661 caagaaggcg gtgaagctgc ccgactatca ctttgtggac caccgcatcg agatcctgaa
      721 ccacgacaag gactacaaca aggtgaccgt ttacgagagc gccgtggccc gcaactccac
      781 cgacggcatg gacgagctgt acaagtccgg actcagatct aagctgaacc ctcctgatga
      841 gagtggcccc ggctgcatga gctgcaagtg tgtgctctcc tgagaattct gcagtcgacg
      901 gtaccgcggg cccgggatcc accggatcta gatactgatc atatca
//
