LOCUS       sequence-85890-e        3992 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesion Kinase, Excitation: 462, Emission: 492.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3992)
  AUTHORS   Ai HW, Olenych SG, Wong P, Davidson MW, Campbell RE
  TITLE     Hue-shifted monomeric variants of Clavularia cyan fluorescent 
            protein: identification of the molecular determinants of color and 
            applications in fluorescence imaging.
  JOURNAL   BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/1741-7007-6-13.
  PUBMED    18325109
REFERENCE   2  (bases 1 to 3992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2008-03-6"; volume: "6:13. doi"; pages: " 
            10.1186/1741-7007-6-13"
COMMENT     Sequence Label: mTFP1-FAK-5
FEATURES             Location/Qualifiers
     source          1..3992
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             35..742
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggagaccac
       61 aatgggcgta atcaagcccg acatgaagat caagctgaag atggagggca acgtgaatgg
      121 ccacgccttc gtgatcgagg gcgagggcga gggcaagccc tacgacggca ccaacaccat
      181 caacctggag gtgaaggagg gagcccccct gcccttctcc tacgacattc tgaccaccgc
      241 gttcgcctac ggcaacaggg ccttcaccaa gtaccccgac gacatcccca actacttcaa
      301 gcagtccttc cccgagggct actcttggga gcgcaccatg accttcgagg acaagggcat
      361 cgtgaaggtg aagtccgaca tctccatgga ggaggactcc ttcatctacg agatacacct
      421 caagggcgag aacttccccc ccaacggccc cgtgatgcag aagaagacca ccggctggga
      481 cgcctccacc gagaggatgt acgtgcgcga cggcgtgctg aagggcgacg tcaagcacaa
      541 gctgctgctg gagggcggcg gccaccaccg cgttgacttc aagaccatct acagggccaa
      601 gaaggcggtg aagctgcccg actatcactt tgtggaccac cgcatcgaga tcctgaacca
      661 cgacaaggac tacaacaagg tgaccgttta cgagagcgcc gtggcccgca actccaccga
      721 cggcatggac gagctgtaca agtccggact cagatctatg gcagcagctt accttgatcc
      781 aaacttgaat catacaccaa gttcaagtgc aaagacgcac ctcggtactg ggatggagcg
      841 ttccccgggg gccatggagc gagtcctaaa ggtttttcac tactttgaaa acagcagcga
      901 gccaacgacg tgggccagca ttatccggca tggagatgct actgatgttc gaggcataat
      961 acagaagatt gtggactgtc acaaagtgaa aaatgtggcc tgctatgggt tgcgactcag
     1021 tcatctgcag tctgaggagg ttcactggct gcacctggac atgggggtat ccaatgtgag
     1081 agagaaattt gaactagcac atcctccaga agaatggaaa tatgaactga gaattcggta
     1141 cctgcccaaa ggatttctaa accagttcac tgaggacaaa ccaactttaa attttttcta
     1201 tcagcaggtg aaaaatgact atatgttaga aatagcagat caagtggacc aggaaattgc
     1261 tttgaaacta ggttgccttg aaatcaggag atcctacgga gagatgagag gcaatgcatt
     1321 agagaagaaa tccaactatg aagtgctaga aaaagatgtc ggtttaagac gattttttcc
     1381 gaagagtttg ctagattcag tgaaggccaa aacactacga aaattaatcc aacagacatt
     1441 tcgacaattt gccaacctca acagagaaga aagtattttg aaattctttg agatcctctc
     1501 tccagtgtac agatttgaca aggaatgctt caagtgtgcc cttggttcaa gctggattat
     1561 ttcagtggag ctggcaattg gcccagagga aggaatcagc taccttacag acaagggtgc
     1621 aaatccaact cacctggcag attttaatca agtacaaact attcagtatt caaacagtga
     1681 agacaaggac agaaaaggga tgttgcaact gaagatagct ggtgcacctg agcctctgac
     1741 agtgacagca ccatccttaa ccattgcaga gaatatggct gacttgatag acggatactg
     1801 ccgactggtg aatggagcca cgcaatcttt tattatcagg ccacagaaag aaggtgaaag
     1861 agctttacca tcaataccaa agctggccaa caatgagaag caaggagtaa ggtcgcacac
     1921 agtctctgta tcagaaacag atgactatgc agagataata gatgaagaag atacttatac
     1981 aatgccatca accagagatt atgaaattca aagggagaga attgaactgg ggcgctgcat
     2041 tggtgaagga cagtttggag atgtgcacca aggaatttac atgagtccgg aaaatccagc
     2101 tatggctgta gcaatcaaaa catgtaaaaa ctgcacctca gacagcgtta gagaaaagtt
     2161 cctacaagaa gccttaacaa tgcgtcagtt tgatcatcct cacattgtga agctcattgg
     2221 agttattaca gaaaacccag tgtggataat catggagctc tgtacacttg gagagttgag
     2281 atcgtttctg caagtaagaa aattcagctt ggacctggcc tccctcatcc tctacgctta
     2341 ccagcttagc acagcacttg cttacctaga gagcaaaaga tttgtacata gagatattgc
     2401 tgctaggaac gtgctggtat ctgccactga ctgtgtgaaa ttgggtgact ttggcttatc
     2461 ccgatacatg gaagacagta cttactataa agcttccaaa ggaaagttac ctatcaaatg
     2521 gatggctcca gagtcaatca acttccgacg gtttacctca gcaagcgatg tgtggatgtt
     2581 tggtgtgtgt atgtgggaga tcctgatgca tggggtaaag cccttccagg gagtgaaaaa
     2641 taatgatgtt attggtcgga ttgagaacgg tgagcggctc cccatgcctc cgaactgccc
     2701 tcccaccctc tacagcctta tgaccaagtg ctgggcatac gaccctagta gacgacccag
     2761 gtttactgaa cttaaagcac aactcagtac aatactggag gaggagaagc tgcagcaaga
     2821 ggaacgaatg agaatggaat ccaggcgaca agtcacagta tcctgggact caggaggatc
     2881 agatgaagct cctcccaagc ccagcaggcc tggttacccc agcccaaggt ccagtgaagg
     2941 gttttatccg agtcctcagc atatggtaca gccaaatcac taccaggtat ctggctactc
     3001 tggttctcat gggataccag ccatggcagg cagcatttat cctgggcaag cttctctctt
     3061 ggatcaaaca gattcctgga accatcgacc tcaggaagta tcagcatggc agccaaacat
     3121 ggaggattcg ggcactttgg atgtacgagg aatggggcag gttctgccca cacatctcat
     3181 ggaggagagg ttaataagac aacagcaaga gatggaagaa gatcaacgct ggcttgagaa
     3241 agaggaacga ttcctggtaa tgaaacctga tgtgcggctc tccagaggca gcattgaacg
     3301 ggaggacgga ggtctccagg gcccagctgg taaccagcac atatatcagc ctgtgggtaa
     3361 accagatcat gccgctccac caaagaagcc ccctcgccct ggagcccccc acttgggcag
     3421 cctcgcgagc ctgaacagcc ccgtggacag ctacaacgaa ggcgtgaaga tcaagccaca
     3481 ggaaatcagc cctcctccta cggccaacct ggaccgctcc aatgacaaag tctatgagaa
     3541 tgtaaccggg ctggtgaaag ctgtcataga gatgtccagt aaaatacagc cagctccgcc
     3601 agaggagtac gtgcccatgg taaaggaggt tggcttggcg ctgagaacct tgctagcaac
     3661 agtggatgag tcgctgccag tgcttcctgc aagcacccac agagagattg agatggccca
     3721 gaaactgctg aactctgacc tggctgagct cattaacaag atgaagctgg cccagcagta
     3781 cgtcatgacc agcctgcagc aggagtacaa gaagcaaatg ctgacggctg ctcacgctct
     3841 ggctgtggat gccaagaact tgctggatgt catcgatcaa gccagactga aaatgatcag
     3901 ccagtccagg cccactaagc tgccgtagcc gaattctgca gatgggatcc accggatcta
     3961 gataactgat cataatcagc cataccacat tg
//
