LOCUS       sequence-85893-e        1382 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 462, Emission: 492.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Ai HW, Olenych SG, Wong P, Davidson MW, Campbell RE
  TITLE     Hue-shifted monomeric variants of Clavularia cyan fluorescent 
            protein: identification of the molecular determinants of color and 
            applications in fluorescence imaging.
  JOURNAL   BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/1741-7007-6-13.
  PUBMED    18325109
REFERENCE   2  (bases 1 to 1382)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2008-03-6"; volume: "6:13. doi"; pages: " 
            10.1186/1741-7007-6-13"
COMMENT     Sequence Label: mTFP1-H1-10
FEATURES             Location/Qualifiers
     source          1..1382
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      627..636
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             633..1343
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
ORIGIN
        1 cagatccgct agccgccacc atgaccgaga actccacctc cgccccggcg gcgaagccca
       61 aacgggccaa ggcttccaag aagtccacgg accaccccaa gtattcagac atgatcgtgg
      121 ctgctatcca ggcagagaag aaccgtgccg gctcctcgcg ccagtccatc caaaagtata
      181 tcaagagcca ctacaaggtg ggtgagaacg ccgactccca gatcaagttg tccatcaagc
      241 gcctagtgac caccggtgtt ctcaagcaaa ccaaaggggt gggcgcctcg gggtccttca
      301 ggctggccaa gggcgatgag cccaaaaggt cggtggcttt caagaagacc aagaaggaag
      361 tcaagaaagt ggccactcca aagaaggcag ccaagcccaa gaaggctgcc tccaaagccc
      421 caagcaagaa acccaaagcc acccctgtca agaaggccaa gaagaagccg gctgccacgc
      481 ccaagaaagc caaaaagccc aaggttgtca aagtcaaacc agtcaaggcc tccaaaccca
      541 agaaggccaa aaccgtgaag cccaaagcca agtcgagtgc caagaggggg agcaagaaga
      601 agtcgggaag cacggatcca ccggtcgcca ccatggtgag caagggcgag gagaccacaa
      661 tgggcgtaat caagcccgac atgaagatca agctgaagat ggagggcaac gtgaatggcc
      721 acgccttcgt gatcgagggc gagggcgagg gcaagcccta cgacggcacc aacaccatca
      781 acctggaggt gaaggaggga gcccccctgc ccttctccta cgacattctg accaccgcgt
      841 tcgcctacgg caacagggcc ttcaccaagt accccgacga catccccaac tacttcaagc
      901 agtccttccc cgagggctac tcttgggagc gcaccatgac cttcgaggac aagggcatcg
      961 tgaaggtgaa gtccgacatc tccatggagg aggactcctt catctacgag atacacctca
     1021 agggcgagaa cttccccccc aacggccccg tgatgcagaa gaagaccacc ggctgggacg
     1081 cctccaccga gaggatgtac gtgcgcgacg gcgtgctgaa gggcgacgtc aagcacaagc
     1141 tgctgctgga gggcggcggc caccaccgcg ttgacttcaa gaccatctac agggccaaga
     1201 aggcggtgaa gctgcccgac tatcactttg tggaccaccg catcgagatc ctgaaccacg
     1261 acaaggacta caacaaggtg accgtttacg agagcgccgt ggcccgcaac tccaccgacg
     1321 gcatggacga gctgtacaag taagcggccg cgactctaga tcataatcag ccataccaca
     1381 tt
//
