LOCUS       sequence-85894-e        1190 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 462, Emission: 492.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Ai HW, Olenych SG, Wong P, Davidson MW, Campbell RE
  TITLE     Hue-shifted monomeric variants of Clavularia cyan fluorescent 
            protein: identification of the molecular determinants of color and 
            applications in fluorescence imaging.
  JOURNAL   BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/1741-7007-6-13.
  PUBMED    18325109
REFERENCE   2  (bases 1 to 1190)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2008-03-6"; volume: "6:13. doi"; pages: " 
            10.1186/1741-7007-6-13"
COMMENT     Sequence Label: mTFP1-H2A-10
FEATURES             Location/Qualifiers
     source          1..1190
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      435..444
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             441..1151
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
ORIGIN
        1 cagatccgct agccgccacc atgtcgggac gtggcaagca gggcggcaaa gctcgcgcca
       61 aggccaagac ccgctcttct cgggccgggc ttcagtttcc cgtaggccga gtgcatcgcc
      121 tgctccgcaa aggcaactat gcggagcggg tcggtgctgg agcgccggtg tacctggcgg
      181 cggtgctgga gtacctgacc gccgagatcc tggagctggc tggcaacgcg gcccgcgaca
      241 acaagaagac tcgcatcatc ccgcgtcacc tccagctggc catccgcaac gatgaggagc
      301 tcaacaagct tctgggcaaa gtcaccatcg cacagggtgg cgtcctgccc aacatccagg
      361 ccgtgctact gcccaagaag accgagagcc accacaaggc gaagggcaag tcgggaagca
      421 cggatccacc ggtcgccacc atggtgagca agggcgagga gaccacaatg ggcgtaatca
      481 agcccgacat gaagatcaag ctgaagatgg agggcaacgt gaatggccac gccttcgtga
      541 tcgagggcga gggcgagggc aagccctacg acggcaccaa caccatcaac ctggaggtga
      601 aggagggagc ccccctgccc ttctcctacg acattctgac caccgcgttc gcctacggca
      661 acagggcctt caccaagtac cccgacgaca tccccaacta cttcaagcag tccttccccg
      721 agggctactc ttgggagcgc accatgacct tcgaggacaa gggcatcgtg aaggtgaagt
      781 ccgacatctc catggaggag gactccttca tctacgagat acacctcaag ggcgagaact
      841 tcccccccaa cggccccgtg atgcagaaga agaccaccgg ctgggacgcc tccaccgaga
      901 ggatgtacgt gcgcgacggc gtgctgaagg gcgacgtcaa gcacaagctg ctgctggagg
      961 gcggcggcca ccaccgcgtt gacttcaaga ccatctacag ggccaagaag gcggtgaagc
     1021 tgcccgacta tcactttgtg gaccaccgca tcgagatcct gaaccacgac aaggactaca
     1081 acaaggtgac cgtttacgag agcgccgtgg cccgcaactc caccgacggc atggacgagc
     1141 tgtacaagta agcggccgcg actctagatc ataatcagcc ataccacatt
//
