LOCUS       sequence-85895-e        1257 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 462, Emission: 492.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Ai HW, Olenych SG, Wong P, Davidson MW, Campbell RE
  TITLE     Hue-shifted monomeric variants of Clavularia cyan fluorescent 
            protein: identification of the molecular determinants of color and 
            applications in fluorescence imaging.
  JOURNAL   BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/1741-7007-6-13.
  PUBMED    18325109
REFERENCE   2  (bases 1 to 1257)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2008-03-6"; volume: "6:13. doi"; pages: " 
            10.1186/1741-7007-6-13"
COMMENT     Sequence Label: mTFP1-H3-23
FEATURES             Location/Qualifiers
     source          1..1257
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      20..29
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    437..489
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      497..506
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             503..1213
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
ORIGIN
        1 gacgtcagat ccgctagccg ccaccatggc tcgtactaaa cagacagctc ggaaatccac
       61 cggcggtaaa gcgccacgca agcagctggc taccaaggct gctcgcaaga gcgcgccggc
      121 taccggcggc gtgaaaaagc ctcaccgtta ccgcccgggc actgtggctc tgcgcgagat
      181 ccgccgctac caaaagtcga ccgagttgct gattcggaag ctgccgttcc agcgcctggt
      241 gcgagaaatc gcccaagact tcaagaccga tcttcgcttc cagagctctg cggtgatggc
      301 gctgcaggag gcttgtgagg cctacttggt agggctcttt gaggacacaa acctttgcgc
      361 catccatgct aagcgagtga ctattatgcc caaagacatc cagctcgctc gccgcattcg
      421 cggagaaaga gcggatctcg agctcaagct tcgaattctg cagtcgacgg taccgcgggc
      481 ccgggatcca ccggtcgcca ccatggtgag caagggcgag gagaccacaa tgggcgtaat
      541 caagcccgac atgaagatca agctgaagat ggagggcaac gtgaatggcc acgccttcgt
      601 gatcgagggc gagggcgagg gcaagcccta cgacggcacc aacaccatca acctggaggt
      661 gaaggaggga gcccccctgc ccttctccta cgacattctg accaccgcgt tcgcctacgg
      721 caacagggcc ttcaccaagt accccgacga catccccaac tacttcaagc agtccttccc
      781 cgagggctac tcttgggagc gcaccatgac cttcgaggac aagggcatcg tgaaggtgaa
      841 gtccgacatc tccatggagg aggactcctt catctacgag atacacctca agggcgagaa
      901 cttccccccc aacggccccg tgatgcagaa gaagaccacc ggctgggacg cctccaccga
      961 gaggatgtac gtgcgcgacg gcgtgctgaa gggcgacgtc aagcacaagc tgctgctgga
     1021 gggcggcggc caccaccgcg ttgacttcaa gaccatctac agggccaaga aggcggtgaa
     1081 gctgcccgac tatcactttg tggaccaccg catcgagatc ctgaaccacg acaaggacta
     1141 caacaaggtg accgtttacg agagcgccgt ggcccgcaac tccaccgacg gcatggacga
     1201 gctgtacaag taagcggccg cgactctaga tcataatcag ccataccaca ttgagaa
//
