LOCUS       sequence-85896-e        1255 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 462, Emission: 492.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Ai HW, Olenych SG, Wong P, Davidson MW, Campbell RE
  TITLE     Hue-shifted monomeric variants of Clavularia cyan fluorescent 
            protein: identification of the molecular determinants of color and 
            applications in fluorescence imaging.
  JOURNAL   BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/1741-7007-6-13.
  PUBMED    18325109
REFERENCE   2  (bases 1 to 1255)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2008-03-6"; volume: "6:13. doi"; pages: " 
            10.1186/1741-7007-6-13"
COMMENT     Sequence Label: mTFP1-H3.3-N-14
FEATURES             Location/Qualifiers
     source          1..1255
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      50..59
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      500..509
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             506..1216
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
ORIGIN
        1 cggtcagatc cgctagcgct accggactca gatctcgagc tcaagcttcg ccaccatggc
       61 ccgaaccaag cagaccgcta ggaagtccac cggtgggaaa gccccccgca aacagctggc
      121 caccaaggcg gctcggaaaa gcgcgccctc taccggcggg gtgaagaagc ctcaccgcta
      181 caggccaggg accgtggctc tgagagagat ccgtcgttac cagaaatcga ctgagctgct
      241 catccggaag ctgccattcc agagattggt gagggagatc gcccaggatt tcaaaaccga
      301 cttgaggttt caaagtgcag ccatcggtgc ccttcaggag gctagcgaag catacctggt
      361 ggggttgttt gaagatacca atctgtgtgc catccacgcc aagagagtca ccatcatgcc
      421 caaagacatc cagttggctc gccggatacg gggggagaga gctgggtccg gaagcgcagg
      481 cagtggggat ccaccggtcg ccaccatggt gagcaagggc gaggagacca caatgggcgt
      541 aatcaagccc gacatgaaga tcaagctgaa gatggagggc aacgtgaatg gccacgcctt
      601 cgtgatcgag ggcgagggcg agggcaagcc ctacgacggc accaacacca tcaacctgga
      661 ggtgaaggag ggagcccccc tgcccttctc ctacgacatt ctgaccaccg cgttcgccta
      721 cggcaacagg gccttcacca agtaccccga cgacatcccc aactacttca agcagtcctt
      781 ccccgagggc tactcttggg agcgcaccat gaccttcgag gacaagggca tcgtgaaggt
      841 gaagtccgac atctccatgg aggaggactc cttcatctac gagatacacc tcaagggcga
      901 gaacttcccc cccaacggcc ccgtgatgca gaagaagacc accggctggg acgcctccac
      961 cgagaggatg tacgtgcgcg acggcgtgct gaagggcgac gtcaagcaca agctgctgct
     1021 ggagggcggc ggccaccacc gcgttgactt caagaccatc tacagggcca agaaggcggt
     1081 gaagctgccc gactatcact ttgtggacca ccgcatcgag atcctgaacc acgacaagga
     1141 ctacaacaag gtgaccgttt acgagagcgc cgtggcccgc aactccaccg acggcatgga
     1201 cgagctgtac aagtaagcgg ccgcgactct agatcataat cagccatacc acatt
//
