LOCUS       sequence-85898-e        2584 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nuclear Envelope, Excitation: 462, Emission: 492.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Ai HW, Olenych SG, Wong P, Davidson MW, Campbell RE
  TITLE     Hue-shifted monomeric variants of Clavularia cyan fluorescent 
            protein: identification of the molecular determinants of color and 
            applications in fluorescence imaging.
  JOURNAL   BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/1741-7007-6-13.
  PUBMED    18325109
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2008-03-6"; volume: "6:13. doi"; pages: " 
            10.1186/1741-7007-6-13"
COMMENT     Sequence Label: mTFP1-LaminB1-7
FEATURES             Location/Qualifiers
     source          1..2584
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1830..1839
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1836..2546
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
ORIGIN
        1 agatccgcta gcgctaccgg actcagatct cgagctcaag cttcgaattc ggtaccatgg
       61 cgactgcgac ccccgtgccg ccgcggatgg gcagccgcgc tggcggcccc accacgccgc
      121 tgagccccac gcgcctgtcg cggctccagg agaaggagga gctgcgcgag ctcaatgacc
      181 ggctggcggt gtacatcgac aaggtgcgca gcctggagac ggagaacagc gcgctgcagc
      241 tgcaggtgac ggagcgcgag gaggtgcgcg gccgtgagct caccggcctc aaggcgctct
      301 acgagaccga gctggccgac gcgcgacgcg cgctcgacga cacggcccgc gagcgcgcca
      361 agctgcagat cgagctgggc aagtgcaagg cggaacacga ccagctgctc ctcaactatg
      421 ctaagaagga atctgatctt aatggcgccc agatcaagct tcgagaatat gaagcagcac
      481 tcaattcgaa agatgcagct cttgctactg cacttggtga caaaaaaagt ttagagggag
      541 atttggagga tctgaaggat cagattgccc agttggaagc ctccttagct gcagccaaaa
      601 aacagttagc agatgaaact ttacttaaag tagatttgga gaatcgttgt cagagcctta
      661 ctgaggactt ggagtttcgc aaaagcatgt atgaagagga gattaacgag accagaagga
      721 agcatgaaac gcgcttggta gaggtggatt ctgggcgtca aattgagtat gagtacaagc
      781 tggcgcaagc ccttcatgag atgagagagc aacatgatgc ccaagtgagg ctgtataagg
      841 aggagctgga gcagacttac catgccaaac ttgagaatgc cagactgtca tcagagatga
      901 atacttctac tgtcaacagt gccagggaag aactgatgga aagccgcatg agaattgaga
      961 gcctttcatc ccagctttct aatctacaga aagagtctag agcatgtttg gaaaggattc
     1021 aagaattaga ggacttgctt gctaaagaaa aagacaactc tcgtcgcatg ctgacagaca
     1081 aagagagaga gatggcggaa ataagggatc aaatgcagca acagctgaat gactatgaac
     1141 agcttcttga tgtaaagtta gccctggaca tggaaatcag tgcttacagg aaactcttag
     1201 aaggcgaaga agagaggttg aagctgtctc caagcccttc ttcccgtgtg acagtatccc
     1261 gagcatcctc aagtcgtagt gtacgtacaa ctagaggaaa gcggaagagg gttgatgtgg
     1321 aagaatcaga ggcgagtagt agtgttagca tctctcattc cgcctcagcc actggaaatg
     1381 tttgcatcga agaaattgat gttgatggga aatttatccg cttgaagaac acttctgaac
     1441 aggatcaacc aatgggaggc tgggagatga tcagaaaaat tggagacaca tcagtcagtt
     1501 ataaatatac ctcaagatat gtgctgaagg caggccagac tgttacaatt tgggctgcaa
     1561 acgctggtgt cacagccagc cccccaactg acctcatctg gaagaaccag aactcgtggg
     1621 gcactggcga agatgtgaag gttatattga aaaattctca gggagaggag gttgctcaaa
     1681 gaagtacagt ctttaaaaca accatacctg aagaagagga ggaggaggaa gaagcagctg
     1741 gagtggttgt tgaggaagaa cttttccacc agcagggaac cccaagagca tccaatagaa
     1801 gctgtgcaat tatggcggat ccaccggtcg ccaccatggt gagcaagggc gaggagacca
     1861 caatgggcgt aatcaagccc gacatgaaga tcaagctgaa gatggagggc aacgtgaatg
     1921 gccacgcctt cgtgatcgag ggcgagggcg agggcaagcc ctacgacggc accaacacca
     1981 tcaacctgga ggtgaaggag ggagcccccc tgcccttctc ctacgacatt ctgaccaccg
     2041 cgttcgccta cggcaacagg gccttcacca agtaccccga cgacatcccc aactacttca
     2101 agcagtcctt ccccgagggc tactcttggg agcgcaccat gaccttcgag gacaagggca
     2161 tcgtgaaggt gaagtccgac atctccatgg aggaggactc cttcatctac gagatacacc
     2221 tcaagggcga gaacttcccc cccaacggcc ccgtgatgca gaagaagacc accggctggg
     2281 acgcctccac cgagaggatg tacgtgcgcg acggcgtgct gaagggcgac gtcaagcaca
     2341 agctgctgct ggagggcggc ggccaccacc gcgttgactt caagaccatc tacagggcca
     2401 agaaggcggt gaagctgccc gactatcact ttgtggacca ccgcatcgag atcctgaacc
     2461 acgacaagga ctacaacaag gtgaccgttt acgagagcgc cgtggcccgc aactccaccg
     2521 acggcatgga cgagctgtac aagtaagcgg ccgcgactct agatcataat cagccatacc
     2581 acat
//
