LOCUS       sequence-85899-e        2556 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nuclear Envelope, Excitation: 462, Emission: 492.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Ai HW, Olenych SG, Wong P, Davidson MW, Campbell RE
  TITLE     Hue-shifted monomeric variants of Clavularia cyan fluorescent 
            protein: identification of the molecular determinants of color and 
            applications in fluorescence imaging.
  JOURNAL   BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/1741-7007-6-13.
  PUBMED    18325109
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2008-03-6"; volume: "6:13. doi"; pages: " 
            10.1186/1741-7007-6-13"
COMMENT     Sequence Label: mTFP1-LaminB1-10
FEATURES             Location/Qualifiers
     source          1..2556
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             35..742
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggagaccac
       61 aatgggcgta atcaagcccg acatgaagat caagctgaag atggagggca acgtgaatgg
      121 ccacgccttc gtgatcgagg gcgagggcga gggcaagccc tacgacggca ccaacaccat
      181 caacctggag gtgaaggagg gagcccccct gcccttctcc tacgacattc tgaccaccgc
      241 gttcgcctac ggcaacaggg ccttcaccaa gtaccccgac gacatcccca actacttcaa
      301 gcagtccttc cccgagggct actcttggga gcgcaccatg accttcgagg acaagggcat
      361 cgtgaaggtg aagtccgaca tctccatgga ggaggactcc ttcatctacg agatacacct
      421 caagggcgag aacttccccc ccaacggccc cgtgatgcag aagaagacca ccggctggga
      481 cgcctccacc gagaggatgt acgtgcgcga cggcgtgctg aagggcgacg tcaagcacaa
      541 gctgctgctg gagggcggcg gccaccaccg cgttgacttc aagaccatct acagggccaa
      601 gaaggcggtg aagctgcccg actatcactt tgtggaccac cgcatcgaga tcctgaacca
      661 cgacaaggac tacaacaagg tgaccgttta cgagagcgcc gtggcccgca actccaccga
      721 cggcatggac gagctgtaca agtccggact cagatctcga gctcaagctt cgatggcgac
      781 tgcgaccccc gtgccgccgc ggatgggcag ccgcgctggc ggccccacca cgccgctgag
      841 ccccacgcgc ctgtcgcggc tccaggagaa ggaggagctg cgcgagctca atgaccggct
      901 ggcggtgtac atcgacaagg tgcgcagcct ggagacggag aacagcgcgc tgcagctgca
      961 ggtgacggag cgcgaggagg tgcgcggccg tgagctcacc ggcctcaagg cgctctacga
     1021 gaccgagctg gccgacgcgc gacgcgcgct cgacgacacg gcccgcgagc gcgccaagct
     1081 gcagatcgag ctgggcaagt gcaaggcgga acacgaccag ctgctcctca actatgctaa
     1141 gaaggaatct gatcttaatg gcgcccagat caagcttcga gaatatgaag cagcactcaa
     1201 ttcgaaagat gcagctcttg ctactgcact tggtgacaaa aaaagtttag agggagattt
     1261 ggaggatctg aaggatcaga ttgcccagtt ggaagcctcc ttagctgcag ccaaaaaaca
     1321 gttagcagat gaaactttac ttaaagtaga tttggagaat cgttgtcaga gccttactga
     1381 ggacttggag tttcgcaaaa gcatgtatga agaggagatt aacgagacca gaaggaagca
     1441 tgaaacgcgc ttggtagagg tggattctgg gcgtcaaatt gagtatgagt acaagctggc
     1501 gcaagccctt catgagatga gagagcaaca tgatgcccaa gtgaggctgt ataaggagga
     1561 gctggagcag acttaccatg ccaaacttga gaatgccaga ctgtcatcag agatgaatac
     1621 ttctactgtc aacagtgcca gggaagaact gatggaaagc cgcatgagaa ttgagagcct
     1681 ttcatcccag ctttctaatc tacagaaaga gtctagagca tgtttggaaa ggattcaaga
     1741 attagaggac ttgcttgcta aagaaaaaga caactctcgt cgcatgctga cagacaaaga
     1801 gagagagatg gcggaaataa gggatcaaat gcagcaacag ctgaatgact atgaacagct
     1861 tcttgatgta aagttagccc tggacatgga aatcagtgct tacaggaaac tcttagaagg
     1921 cgaagaagag aggttgaagc tgtctccaag cccttcttcc cgtgtgacag tatcccgagc
     1981 atcctcaagt cgtagtgtac gtacaactag aggaaagcgg aagagggttg atgtggaaga
     2041 atcagaggcg agtagtagtg ttagcatctc tcattccgcc tcagccactg gaaatgtttg
     2101 catcgaagaa attgatgttg atgggaaatt tatccgcttg aagaacactt ctgaacagga
     2161 tcaaccaatg ggaggctggg agatgatcag aaaaattgga gacacatcag tcagttataa
     2221 atatacctca agatatgtgc tgaaggcagg ccagactgtt acaatttggg ctgcaaacgc
     2281 tggtgtcaca gccagccccc caactgacct catctggaag aaccagaact cgtggggcac
     2341 tggcgaagat gtgaaggtta tattgaaaaa ttctcaggga gaggaggttg ctcaaagaag
     2401 tacagtcttt aaaacaacca tacctgaaga agaggaggag gaggaagaag cagctggagt
     2461 ggttgttgag gaagaacttt tccaccagca gggaacccca agagcatcca atagaagctg
     2521 tgcaattatg tagggatcca ccggatctag ataact
//
