LOCUS       sequence-85900-e        1336 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Light Chain Myosin, Excitation: 462, Emission: 492.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Ai HW, Olenych SG, Wong P, Davidson MW, Campbell RE
  TITLE     Hue-shifted monomeric variants of Clavularia cyan fluorescent 
            protein: identification of the molecular determinants of color and 
            applications in fluorescence imaging.
  JOURNAL   BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/1741-7007-6-13.
  PUBMED    18325109
REFERENCE   2  (bases 1 to 1336)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2008-03-6"; volume: "6:13. doi"; pages: " 
            10.1186/1741-7007-6-13"
COMMENT     Sequence Label: mTFP1-LC-Myosin-N-7
FEATURES             Location/Qualifiers
     source          1..1336
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      581..590
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             587..1297
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggactc agatctcgag ctcaagctta tgtcgagcaa
       61 gagagccaag gccaagacca ccaagaagag gccccagagg gctacgtcca atgtcttcgc
      121 catgtttgac cagtcccaga tccaggagtt taaggaggcc ttcaacatga ttgatcagaa
      181 ccgcgatggc ttcattgata aggaggacct gcacgacatg ttggcctctc tggggaagaa
      241 ccccacagac gagtacctgg agggcatgat gaacgaggcg ccagggccca tcaacttcac
      301 catgttcctc acaatgtttg gggagaagct gaacggcaca gaccccgagg atgtgatccg
      361 caatgccttt gcctgctttg atgaggaggc ctcaggcttc atccacgagg accacctgag
      421 ggagctgctc accaccatgg gcgaccgatt cacggatgag gaggtggacg agatgtaccg
      481 cgaggcaccc attgataaga agggcaactt caactacgtg gagttcactc gcatcctcaa
      541 acacggcgcc aaggacaagg acgacctgga tccaccggtc gccaccatgg tgagcaaggg
      601 cgaggagacc acaatgggcg taatcaagcc cgacatgaag atcaagctga agatggaggg
      661 caacgtgaat ggccacgcct tcgtgatcga gggcgagggc gagggcaagc cctacgacgg
      721 caccaacacc atcaacctgg aggtgaagga gggagccccc ctgcccttct cctacgacat
      781 tctgaccacc gcgttcgcct acggcaacag ggccttcacc aagtaccccg acgacatccc
      841 caactacttc aagcagtcct tccccgaggg ctactcttgg gagcgcacca tgaccttcga
      901 ggacaagggc atcgtgaagg tgaagtccga catctccatg gaggaggact ccttcatcta
      961 cgagatacac ctcaagggcg agaacttccc ccccaacggc cccgtgatgc agaagaagac
     1021 caccggctgg gacgcctcca ccgagaggat gtacgtgcgc gacggcgtgc tgaagggcga
     1081 cgtcaagcac aagctgctgc tggagggcgg cggccaccac cgcgttgact tcaagaccat
     1141 ctacagggcc aagaaggcgg tgaagctgcc cgactatcac tttgtggacc accgcatcga
     1201 gatcctgaac cacgacaagg actacaacaa ggtgaccgtt tacgagagcg ccgtggcccg
     1261 caactccacc gacggcatgg acgagctgta caagtaagcg gccgcgactc tagatcataa
     1321 tcagccatac cacatt
//
