LOCUS       sequence-85901-e        2083 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Lysosome Membrane, Excitation: 462, Emission: 492.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ai HW, Olenych SG, Wong P, Davidson MW, Campbell RE
  TITLE     Hue-shifted monomeric variants of Clavularia cyan fluorescent 
            protein: identification of the molecular determinants of color and 
            applications in fluorescence imaging.
  JOURNAL   BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/1741-7007-6-13.
  PUBMED    18325109
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2008-03-6"; volume: "6:13. doi"; pages: " 
            10.1186/1741-7007-6-13"
COMMENT     Sequence Label: mTFP1-Lysosomes-20
FEATURES             Location/Qualifiers
     source          1..2083
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      41..50
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      45..54
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(47..109,110..1267)
                     /codon_start=1
                     /product="rat lysosome-associated membrane glycoprotein 1"
                     /label=LAMP1
                     /translation="MAAPGARRPLLLLLLAGLAHSAPALFEVKDNNGTACIMASFSASF
                     LTTYEAGHVSKVSNMTLPASAEVLKNSSSCGEKNASEPTLAITFGEGYLLKLTFTKNTT
                     RYSVQHMYFTYNLSDTQFFPNASSKGPDTVDSTTDIKADINKTYRCVSDIRVYMKNVTI
                     VLWDATIQAYLPSSNFSKEETRCPQDQPSPTTGPPSPSPPLVPTNPSVSKYNVTGDNGT
                     CLLASMALQLNITYMKKDNTTVTRAFNINPSDKYSGTCGAQLVTLKVGNKSRVLELQFG
                     MNATSSLFFLQGVQLNMTLPDAIEPTFSTSNYSLKALQASVGNSYKCNSEEHIFVSKAL
                     ALNVFSVQVQAFRVESDRFGSVEECVQDGNNMLIPIAVGGALAGLVLIVLIAYLIGRKR
                     SHAGYQTI"
     regulatory      1322..1331
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1328..2038
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
ORIGIN
        1 acgtcagatc cgctagcgct accggactca gatctcgagc gccaccatgg cggccccggg
       61 cgcccggcgg ccgctgctcc tgttgctgct ggcaggcctt gcacacagcg ccccagcact
      121 gttcgaggtg aaagacaaca acggcacagc gtgtataatg gccagcttct ctgcctcctt
      181 tctgaccacc tatgaggctg gacatgtttc taaggtctcg aatatgaccc tgccagcctc
      241 tgcagaagtc ctgaagaata gcagctcttg tggtgaaaag aatgcttctg agcccaccct
      301 cgcaatcacc tttggagaag gatatttact gaaactcacc ttcacaaaaa acacaacacg
      361 ttacagtgtc cagcacatgt atttcacata taacctgtca gacacacaat tctttcccaa
      421 tgccagctcc aaagggcccg acactgtgga ttccacaact gacatcaagg cagacatcaa
      481 caaaacatac cgatgtgtca gcgacatcag ggtctacatg aagaatgtga ccattgtgct
      541 ctgggacgct actatccagg cctacctgcc gagtagcaac ttcagcaagg aagagacacg
      601 ctgcccacag gatcaacctt ccccaactac tgggccaccc agcccctcac caccacttgt
      661 gcccacaaac cccagtgtgt ccaagtacaa tgtgactggt gacaatggaa cctgcctgct
      721 ggcctctatg gcactgcaac tcaacatcac ctacatgaag aaggacaaca cgactgtgac
      781 cagagcattc aacatcaacc caagtgacaa atatagtggg acttgcggtg cccagttggt
      841 gaccctgaag gtggggaaca agagcagagt cctggagctg cagtttggga tgaatgccac
      901 ttctagcctg tttttcctgc aaggagttca gttgaacatg actcttcctg atgccataga
      961 gcccacgttc agcacctcca actattccct gaaagctctt caggccagtg tcggcaactc
     1021 atacaagtgc aacagtgagg agcacatctt tgtcagcaag gcgctcgccc tcaatgtctt
     1081 cagcgtgcaa gtccaggctt tcagggtaga aagtgacagg tttgggtctg tggaagagtg
     1141 tgtacaggac ggtaacaaca tgctgatccc cattgctgtg ggcggggccc tggcagggct
     1201 ggtcctcatc gtcctcatcg cctacctcat cggcaggaag aggagtcacg cgggctatca
     1261 gaccatctcg gaattcggct ccaccggctc caccggctcc accggcgcgg atccaccggt
     1321 cgccaccatg gtgagcaagg gcgaggagac cacaatgggc gtaatcaagc ccgacatgaa
     1381 gatcaagctg aagatggagg gcaacgtgaa tggccacgcc ttcgtgatcg agggcgaggg
     1441 cgagggcaag ccctacgacg gcaccaacac catcaacctg gaggtgaagg agggagcccc
     1501 cctgcccttc tcctacgaca ttctgaccac cgcgttcgcc tacggcaaca gggccttcac
     1561 caagtacccc gacgacatcc ccaactactt caagcagtcc ttccccgagg gctactcttg
     1621 ggagcgcacc atgaccttcg aggacaaggg catcgtgaag gtgaagtccg acatctccat
     1681 ggaggaggac tccttcatct acgagataca cctcaagggc gagaacttcc cccccaacgg
     1741 ccccgtgatg cagaagaaga ccaccggctg ggacgcctcc accgagagga tgtacgtgcg
     1801 cgacggcgtg ctgaagggcg acgtcaagca caagctgctg ctggagggcg gcggccacca
     1861 ccgcgttgac ttcaagacca tctacagggc caagaaggcg gtgaagctgc ccgactatca
     1921 ctttgtggac caccgcatcg agatcctgaa ccacgacaag gactacaaca aggtgaccgt
     1981 ttacgagagc gccgtggccc gcaactccac cgacggcatg gacgagctgt acaagtaagc
     2041 ggccgcgact ctagatcata atcagccata ccacattgta gag
//
