LOCUS       sequence-85902-e        2077 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Lysosome Membrane, Excitation: 462, Emission: 492.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2077)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mTFP1-Lysosomes-M-20
FEATURES             Location/Qualifiers
     source          1..2077
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      42..51
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      46..55
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(48..110,111..1268)
                     /codon_start=1
                     /product="rat lysosome-associated membrane glycoprotein 1"
                     /label=LAMP1
                     /translation="MAAPGARRPLLLLLLAGLAHSAPALFEVKDNNGTACIMASFSASF
                     LTTYEAGHVSKVSNMTLPASAEVLKNSSSCGEKNASEPTLAITFGEGYLLKLTFTKNTT
                     RYSVQHMYFTYNLSDTQFFPNASSKGPDTVDSTTDIKADINKTYRCVSDIRVYMKNVTI
                     VLWDATIQAYLPSSNFSKEETRCPQDQPSPTTGPPSPSPPLVPTNPSVSKYNVTGDNGT
                     CLLASMALQLNITYMKKDNTTVTRAFNINPSDKYSGTCGAQLVTLKVGNKSRVLELQFG
                     MNATSSLFFLQGVQLNMTLPDAIEPTFSTSNYSLKALQASVGNSYKCNSEEHIFVSKAL
                     ALNVFSVQVQAFRVESDRFGSVEECVQDGNNMLIPIAVGGALAGLVLIVLIAYLIGRKR
                     SHAGYQTI"
     regulatory      1323..1332
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1329..2039
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
ORIGIN
        1 acggtcagat ccgctagcgc taccggactc agatctcgag cgccaccatg gcggccccgg
       61 gcgcccggcg tccgctgctc ctgttgctgc tggcaggcct tgcacacagc gccccagcac
      121 tgttcgaggt gaaagacaac aacggcacag cgtgtataat ggccagcttc tctgcctcct
      181 ttctgaccac ctatgaggct ggacatgttt ctaaggtctc gaatatgacc ctgccagcct
      241 ctgcagaagt cctgaagaat agcagctctt gtggtgaaaa gaatgcttct gagcccaccc
      301 tcgcaatcac ctttggagaa ggatatttac tgaaactcac cttcacaaaa aacacaacac
      361 gttacagtgt ccagcacatg tatttcacat ataacctgtc agacacacaa ttctttccca
      421 atgccagctc caaagggccc gacactgtgg attccacaac tgacatcaag gcagacatca
      481 acaaaacata ccgatgtgtc agcgacatca gggtctacat gaagaatgtg accattgtgc
      541 tctgggacgc tactatccag gcctacctgc cgagtagcaa cttcagcaag gaagagacac
      601 gctgcccaca ggatcaacct tccccaacta ctgggccacc cagcccctca ccaccacttg
      661 tgcccacaaa ccccagtgtg tccaagtaca atgtgactgg tgacaatgga acctgcctgc
      721 tggcctctat ggcactgcaa ctcaacatca cctacatgaa gaaggacaac acgactgtga
      781 ccagagcatt caacatcaac ccaagtgaca aatatagtgg gacttgcggt gcccagttgg
      841 tgaccctgaa ggtggggaac aagagcagag tcctggagct gcagtttggg atgaatgcca
      901 cttctagcct gtttttcctg caaggagttc agttgaacat gactcttcct gatgccatag
      961 agcccacgtt cagcacctcc aactattccc tgaaagctct tcaggccagt gtcggcaact
     1021 catacaagtg caacagtgag gagcacatct ttgtcagcaa ggcgctcgcc ctcaatgtct
     1081 tcagcgtgca agtccaggct ttcagggtag aaagtgacag gtttgggtct gtggaagagt
     1141 gtgtacagga cggtaacaac atgctgatcc ccattgctgt gggcggggcc ctggcagggc
     1201 tggtcctcat cgtcctcatc gcctacctca tcggcaggaa gaggagtcac gcgggctatc
     1261 agaccatctc ggaattcggc tccaccggct ccaccggctc caccggcgcg gatccaccgg
     1321 tcgccaccat ggtgagcaag ggcgaggaga ccacaatggg cgtaatcaag cccgacatga
     1381 agatcaagct gaagatggag ggcaacgtga atggccacgc cttcgtgatc gagggcgagg
     1441 gcgagggcaa gccctacgac ggcaccaaca ccatcaacct ggaggtgaag gagggagccc
     1501 ccctgccctt ctcctacgac attctgacca ccgcgttcgc ctacggcaac agggccttca
     1561 ccaagtaccc cgacgacatc cccaactact tcaagcagtc cttccccgag ggctactctt
     1621 gggagcgcac catgaccttc gaggacaagg gcatcgtgaa ggtgaagtcc gacatctcca
     1681 tggaggagga ctccttcatc tacgagatac acctcaaggg cgagaacttc ccccccaacg
     1741 gccccgtgat gcagaagaag accaccggct gggacgcctc caccgagagg atgtacgtgc
     1801 gcgacggcgt gctgaagggc gacgtcaagc acaagctgct gctggagggc ggcggccacc
     1861 accgcgttga cttcaagacc atctacaggg ccaagaaggc ggtgaagctg cccgactatc
     1921 actttgtgga ccaccgcatc gagatcctga accacgacaa ggactacaac aaggtgaccg
     1981 tttacgagag cgccgtggcc cgcaactcca ccgacggcat ggacgagctg tacaagtaag
     2041 cggccgcgac tctagatcat aatcagccat accacat
//
