LOCUS       sequence-85903-e        1134 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Mannosidase/Golgi, Excitation: 462, Emission: 492.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Ai HW, Olenych SG, Wong P, Davidson MW, Campbell RE
  TITLE     Hue-shifted monomeric variants of Clavularia cyan fluorescent 
            protein: identification of the molecular determinants of color and 
            applications in fluorescence imaging.
  JOURNAL   BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/1741-7007-6-13.
  PUBMED    18325109
REFERENCE   2  (bases 1 to 1134)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2008-03-6"; volume: "6:13. doi"; pages: " 
            10.1186/1741-7007-6-13"
COMMENT     Sequence Label: mTFP1-MannII-N-10
FEATURES             Location/Qualifiers
     source          1..1134
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      378..387
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             384..1094
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
ORIGIN
        1 gatccgctag cgccaccatg aagttaagtc gccagttcac cgtgtttggc agcgcgatct
       61 tctgcgtcgt aatcttctca ctctacctga tgctggacag gggtcacttg gactaccctc
      121 ggggcccgcg ccaggagggc tcctttccgc agggccagct ttcaatattg caagaaaaga
      181 ttgaccattt ggagcgtttg ctcgctgaga acaacgagat catctcaaat atcagagact
      241 cagtcatcaa cctgagcgag tctgtggagg acggcccgcg ggggtcacca ggcaacgcca
      301 gccaaggctc catccacctc cactcgccac agttggccct gcaggctgac cccagctccg
      361 gatcggatcc accggtcgcc accatggtga gcaagggcga ggagaccaca atgggcgtaa
      421 tcaagcccga catgaagatc aagctgaaga tggagggcaa cgtgaatggc cacgccttcg
      481 tgatcgaggg cgagggcgag ggcaagccct acgacggcac caacaccatc aacctggagg
      541 tgaaggaggg agcccccctg cccttctcct acgacattct gaccaccgcg ttcgcctacg
      601 gcaacagggc cttcaccaag taccccgacg acatccccaa ctacttcaag cagtccttcc
      661 ccgagggcta ctcttgggag cgcaccatga ccttcgagga caagggcatc gtgaaggtga
      721 agtccgacat ctccatggag gaggactcct tcatctacga gatacacctc aagggcgaga
      781 acttcccccc caacggcccc gtgatgcaga agaagaccac cggctgggac gcctccaccg
      841 agaggatgta cgtgcgcgac ggcgtgctga agggcgacgt caagcacaag ctgctgctgg
      901 agggcggcgg ccaccaccgc gttgacttca agaccatcta cagggccaag aaggcggtga
      961 agctgcccga ctatcacttt gtggaccacc gcatcgagat cctgaaccac gacaaggact
     1021 acaacaaggt gaccgtttac gagagcgccg tggcccgcaa ctccaccgac ggcatggacg
     1081 agctgtacaa gtaagcggcc gcgactctag atcataatca gccataccac attt
//
